Fermentation of Detoxified Acid-Hydrolyzed Pyrolytic Anhydrosugars into Bioethanol with Saccharomyces cerevisiae 2.399


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Pyrolysate obtained from the pyrolysis of waste cotton is a source of fermentable sugars that could be fermented into bioethanol fuel and other chemicals via microbial fermentation. However, pyrolysate is a complex mixture of fermentable and non-fermentable substrates causing inhibition of the microbial growth. The aim of this study was to detoxify the hydrolysate and then ferment it into bio-ethanol fuel in shake flasks and fermenter applying yeast strain Saccharomyces cerevisiae 2.399. Pyrolysate was hydrolyzed to glucose with 0.2 M sulfuric acid, neutralized with Ba(OH)2 followed by treatment with ethyl acetate and activated carbon to remove fermentation inhibitors. The effect of various fermentation parameters such as inoculum concentration, pH and hydrolysate glucose was evaluated in shake flasks for optimum ethanol fermentation. With respect to inoculum concentration, 20% v/v inoculum i.e. 8.0 × 108–1.2 × 109 cells/mL was the optimum level for producing 8.62 ± 0.33 g/L ethanol at 9 h of fermentation with a maximum yield of 0.46 g ethanol/g glucose. The optimum pH for hydrolysate glucose fermentation was found to be 6.0 that produced 8.57 ± 0.66 g/L ethanol. Maximum ethanol concentration, 14.78 g/L was obtained for 4% hydrolysate glucose concentration after 16 h of fermentation. Scale-up studies in stirred fermenter produced much higher productivity (1.32 g/L/h–1) compared to shake flask fermentation (0.92 g/L/h–1). The yield of ethanol reached a maximum of 91% and 89% of the theoretical yield of ethanol in shake flasks and fermenter, respectively. The complex of integrated models of development was applied, that has been successfully tested previously for the mathematical analysis of the fermentation processes.

作者简介

Z. Islam

College of Resources and Environment

Email: yuzs@ucas.ac.cn
中国, Beijing, 100049

S. Klykov

Alpha Integrum Ltd.

Email: yuzs@ucas.ac.cn
俄罗斯联邦, Protvino, Moscow oblast, 142281

Z. Yu

College of Resources and Environment

编辑信件的主要联系方式.
Email: yuzs@ucas.ac.cn
中国, Beijing, 100049

D. Chang

College of Resources and Environment

Email: yuzs@ucas.ac.cn
中国, Beijing, 100049

E. Hassan

Department of Sustainable Bioproducts

Email: yuzs@ucas.ac.cn
美国, Mississippi State, MS, 39762

H. Zhang

College of Resources and Environment

Email: yuzs@ucas.ac.cn
中国, Beijing, 100049

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