电邮这篇文章 Compatibility of cells of the nervous system with structured biodegradable chitosan-based hydrogel matrices
- 作者: Timashev P.S.1, Bardakova K.N.1, Minaev N.V.1, Demina T.S.2, Mishchenko T.A.3, Mitroshina E.V.3, Akovantseva A.A.1, Koroleva A.V.1, Asyutin D.S.4, Pimenova L.F.4, Konovalov N.A.4, Akopova T.A.2, Solov’eva A.B.5, Mukhina I.V.3, Vedunova M.V.6, Chichkov B.N.1, Bagratashvili V.N.1
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隶属关系:
- Institute of Laser and Information Technologies, Department of Advanced Laser Technology
- Enikolopov Institute of Synthetic Polymeric Materials
- Nizhny Novgorod State Medical Academy
- Burdenko Research Institute of Neurosurgery
- Semenov Institute of Chemical Physics
- Institute of Biology and Biomedicine
- 期: 卷 52, 编号 5 (2016)
- 页面: 508-514
- 栏目: Article
- URL: https://journal-vniispk.ru/0003-6838/article/view/152009
- DOI: https://doi.org/10.1134/S0003683816050161
- ID: 152009
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详细
Hydrogel matrices for cell cultivation have been generated by two-photon laser polymerization of unsaturated chitosan derivatives and methacrylated hyaluronic acid. The adhesive and toxic properties of the matrices have been assessed, and the matrices have been shown to have a good compatibility with primary hippocampal cell cultures. The formation of morphologically normal neural networks by cells of the nervous system cultured on the surface of hydrogel matrices has been observed. The metabolic status of dissociated hippocampal cells cultured on the matrices was similar to that of the control cultures, as shown by the results of MTT reductase activity assay. Thus, matrices based on unsaturated polysaccharide derivatives crosslinked by laser irradiation showed good compatibility with differentiated cells of the nervous system and considerable potential for use in neurotransplantation.
作者简介
P. Timashev
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
编辑信件的主要联系方式.
Email: timashev.peter@gmail.com
俄罗斯联邦, Troitsk, Moscow, 108840
K. Bardakova
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
俄罗斯联邦, Troitsk, Moscow, 108840
N. Minaev
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
俄罗斯联邦, Troitsk, Moscow, 108840
T. Demina
Enikolopov Institute of Synthetic Polymeric Materials
Email: timashev.peter@gmail.com
俄罗斯联邦, Moscow, 117393
T. Mishchenko
Nizhny Novgorod State Medical Academy
Email: timashev.peter@gmail.com
俄罗斯联邦, Nizhny Novgorod, 603005
E. Mitroshina
Nizhny Novgorod State Medical Academy
Email: timashev.peter@gmail.com
俄罗斯联邦, Nizhny Novgorod, 603005
A. Akovantseva
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
俄罗斯联邦, Troitsk, Moscow, 108840
A. Koroleva
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
俄罗斯联邦, Troitsk, Moscow, 108840
D. Asyutin
Burdenko Research Institute of Neurosurgery
Email: timashev.peter@gmail.com
俄罗斯联邦, Moscow, 125047
L. Pimenova
Burdenko Research Institute of Neurosurgery
Email: timashev.peter@gmail.com
俄罗斯联邦, Moscow, 125047
N. Konovalov
Burdenko Research Institute of Neurosurgery
Email: timashev.peter@gmail.com
俄罗斯联邦, Moscow, 125047
T. Akopova
Enikolopov Institute of Synthetic Polymeric Materials
Email: timashev.peter@gmail.com
俄罗斯联邦, Moscow, 117393
A. Solov’eva
Semenov Institute of Chemical Physics
Email: timashev.peter@gmail.com
俄罗斯联邦, Moscow, 119991
I. Mukhina
Nizhny Novgorod State Medical Academy
Email: timashev.peter@gmail.com
俄罗斯联邦, Nizhny Novgorod, 603005
M. Vedunova
Institute of Biology and Biomedicine
Email: timashev.peter@gmail.com
俄罗斯联邦, Nizhny Novgorod, 603950
B. Chichkov
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
俄罗斯联邦, Troitsk, Moscow, 108840
V. Bagratashvili
Institute of Laser and Information Technologies, Department of Advanced Laser Technology
Email: timashev.peter@gmail.com
俄罗斯联邦, Troitsk, Moscow, 108840
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