New recombinant strains of the yeast Yarrowia lipolytica with overexpression of the aconitate hydratase gene for the obtainment of isocitric acid from rapeseed oil


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

The yeast Yarrowia lipolytica is capable of high-intensity synthesis (overproduction) of citric (CA) and isocitric (ICA) acids under nitrogen limitation. The ratio of the synthesized acids depends on the producing strains used and the expression level of the aconitate hydratase gene (ACO1). Recombinant variants with overexpression of the multicopy ACO1 gene have been obtained based on the natural ICA-producing strain Y. lipolytica 672. A recombinant strain Y. lipolytica 20, which has an isocitrate-citrate ratio shifted towards ICA (2.3: 1) as compared to the parental strain (1.1: 1), has been selected. Culturing of the 20 variant in a 10 L reactor has resulted in the production of 72.6 g/L of ICA and 29.0 g/L of CA with a ratio of 2.5: 1. This makes it possible to regard Y. lipolytica 20 as a promising producer for the development of an industrial process for isocitrate production.

Авторлар туралы

I. Laptev

State Research Institute of Genetics and Selection of Industrial Microorganisms

Хат алмасуға жауапты Автор.
Email: ivanlaptev@mail.ru
Ресей, Moscow, 117545

N. Filimonova

State Research Institute of Genetics and Selection of Industrial Microorganisms

Email: ivanlaptev@mail.ru
Ресей, Moscow, 117545

R. Allayarov

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: ivanlaptev@mail.ru
Ресей, Pushchino, 142290

S. Kamzolova

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: ivanlaptev@mail.ru
Ресей, Pushchino, 142290

V. Samoilenko

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: ivanlaptev@mail.ru
Ресей, Pushchino, 142290

S. Sineoky

State Research Institute of Genetics and Selection of Industrial Microorganisms

Email: ivanlaptev@mail.ru
Ресей, Moscow, 117545

I. Morgunov

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: ivanlaptev@mail.ru
Ресей, Pushchino, 142290

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML

© Pleiades Publishing, Inc., 2016