Chemical Modification of Fusion Protein Based on the Thermus thermophilus GroEL Chaperon with AEBSF Protease Inhibitor
- Authors: Zenin V.A.1,2, Novikova L.A.2, Yurkova M.S.1,2, Savvin I.O.1, Kurov K.A.2, Fedorov A.N.1,2
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Affiliations:
- Peoples’ Friendship University of Russia
- Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
- Issue: Vol 55, No 6 (2019)
- Pages: 649-653
- Section: Article
- URL: https://journal-vniispk.ru/0003-6838/article/view/153046
- DOI: https://doi.org/10.1134/S0003683819060164
- ID: 153046
Cite item
Abstract
Protease inhibitors are routinely used to prepare functional, full-size proteins. Here, we describe modifications of the chimeric protein based on the GroEL chaperon from Thermus thermophilus. Modifications of this chimeric protein resulted from its interaction during sample preparation with an irreversible inhibitor of serine proteases, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF), which belongs to the sulfonyl-fluoride class of compounds. Protein samples were then identified via MALDI-TOF/TOF after in-gel preparation with trypsin. Modifications of tyrosine and lysine amino acid residues were shown to be present. Also, the availability of the tyrosine residue was found to be a prerequisite for its modification.
About the authors
V. A. Zenin
Peoples’ Friendship University of Russia; Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198; Moscow, 119071
L. A. Novikova
Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 119071
M. S. Yurkova
Peoples’ Friendship University of Russia; Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198; Moscow, 119071
I. O. Savvin
Peoples’ Friendship University of Russia
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198
K. A. Kurov
Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 119071
A. N. Fedorov
Peoples’ Friendship University of Russia; Fundamentals of Biotechnology Federal Research Center, Russian Academy of Sciences
Author for correspondence.
Email: a.fedorov@fbras.ru
Russian Federation, Moscow, 117198; Moscow, 119071
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