Production of humanized F(ab’) 2  fragment of rabies blocking antibodies in  Pichia pastoris  yeast

T. A. Yagudin; E. V. Klyatchko; S. S. Zatsepin; E. V. Morozkina; S. V. Benevolensky; O. B. Shemchukova; L. P. Pozdnyakova; O. N. Solopova; P. G. Sveshnikov

doi:10.1134/S0003683816040165

Production of humanized F(ab’)₂ fragment of rabies blocking antibodies in Pichia pastoris yeast

Authors: Yagudin T.A.¹, Klyatchko E.V.¹, Zatsepin S.S.¹, Morozkina E.V.¹, Benevolensky S.V.¹, Shemchukova O.B.², Pozdnyakova L.P.², Solopova O.N.², Sveshnikov P.G.²
Affiliations:
1. Bach Institute of Biochemistry
2. All-Russia Scientific Center of Molecular Diagnostics and Treatment
Issue: Vol 52, No 4 (2016)
Pages: 378-383
Section: Article
URL: https://journal-vniispk.ru/0003-6838/article/view/151953
DOI: https://doi.org/10.1134/S0003683816040165
ID: 151953

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Abstract

The yeast strain Pichia pastoris, a producer of humanized F(ab’)₂ fragments of rabies-blocking antibodies, has been obtained. Human chaperone BiP coexpression caused a twofold increase of the immunoglobulins secretion level. The use of Fos and Jun zippers in the composition of heavy chains facilitated the dimerization of F(ab’)₂ fragments of the shared pool of secreted immunoglobulins up to 75%.