Biosynthesis of amylase inhibitor by Streptomycete cultures

Resumo

We studied the effects of different sources of carbon, nitrogen, and dietary elements on the biosynthesis of amylase inhibitor produced by two strains from the Russian National Collection of Industrial Microorganisms: Streptomyces violaceus (code: Ac-1734) and Streptomyces lucensis (Ac-1743). We found that the biosynthesis of amylase inhibitor can be regulated by limiting carbon and oxygen concentrations, maintaining constant a C: N ratio and a stable proportion of carbohydrates (dextrins, maltose and glucose) in the medium, and by adding an extra organic source of nitrogen to the medium. The inhibitors produced by the cultures are pseudo-polysaccharides.