Plant protein hydrolysates as fish fry feed in aquaculture. Hydrolysis of rapeseed proteins by an enzyme complex from king crab hepatopancreas
- 作者: Muranova T.A.1, Zinchenko D.V.1, Kononova S.V.2, Belova N.A.1, Miroshnikov A.I.1
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隶属关系:
- Faculty of Biotechnology
- Institute of Protein Research
- 期: 卷 53, 编号 6 (2017)
- 页面: 680-687
- 栏目: Article
- URL: https://journal-vniispk.ru/0003-6838/article/view/152339
- DOI: https://doi.org/10.1134/S0003683817060102
- ID: 152339
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详细
Rapeseed proteins were processed by an enzyme complex isolated from king crab hepatopancreas in order to obtain a hydrolysate for use as fish fry feed. The amino acid composition of the obtained protein preparation was close to the amino acid composition of fishmeal traditionally used in the production of fish feed. SDS-PAGE, HPLC, and mass spectrometric analysis of the products of enzymatic hydrolysis of rapeseed proteins showed that the proteins were hydrolyzed to a high degree. The composition of the hydrolysates depended on the hydrolysis time and included free amino acids (27% of the total weight of the protein mix after 3 h of hydrolysis and 56% after 21 h of hydrolysis), short peptides (2 to 20 amino acid residues), and small amounts of protein fragments with a molecular weight of approximately 14 kDa, as shown by by SDS-PAG electrophoresis.
作者简介
T. Muranova
Faculty of Biotechnology
Email: zdv@bibch.ru
俄罗斯联邦, Moscow, 119991
D. Zinchenko
Faculty of Biotechnology
编辑信件的主要联系方式.
Email: zdv@bibch.ru
俄罗斯联邦, Moscow, 119991
S. Kononova
Institute of Protein Research
Email: zdv@bibch.ru
俄罗斯联邦, Pushchino, Moscow oblast, 142290
N. Belova
Faculty of Biotechnology
Email: zdv@bibch.ru
俄罗斯联邦, Moscow, 119991
A. Miroshnikov
Faculty of Biotechnology
Email: zdv@bibch.ru
俄罗斯联邦, Moscow, 119991
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