Molecular Genetic and Functional Analysis of the Genome of Bacteria Bacillus velezensis BIM B-439D

Resumo

Whole-genome sequencing of bacteria Bacillus velezensis BIM B-439D has revealed that its genome is represented by a single circular chromosome 3 978 954 bp in size and contains 3969 predicted genes. Chromosome has 10 conserved loci that determine the production of antimicrobial compounds of varying chemical nature, namely lipopeptides (surfactin, bacillomycin, fengycin), polyketides (bacillaene, difficidin/oxydifficidin, macrolactin), bacillibactin siderophore, dipeptide bacilysin, a protein/polyketide of uncertain composition, and amylocyclicin bacteriocin. Mutants with impaired surfactin synthesis have been obtained by direct mutagenesis; they were characterized by an increased production of bacillomycin and fengycin. The obtained mutants demonstrated high antimicrobial activity towards a number of bacterial and fungal pathogens (Penicillium expansum, Alternaria tenuis, Botrytis cinerea,Bipolaris sorokiniana), but they inhibited the growth of the Fusarium genus fungi to a lesser degree.