Evaluation of Efficacy of White Blood Cell Identification in Peripheral Blood by Automated Scanning of Stained Blood Smear Images with Variable Magnification


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Abstract

Development of blood smear scanner analyzers is a new promising trend in designing devices for blood cell morphology examination. The efficacy of using the Vision Hema® Ultimate scanner analyzer for differential white blood cell count and identification of nucleated blood cells was evaluated. The average time required to analyze a single cover-glass preparation was found to be (108 ± 17)″. There was no statistically significant difference between the results of differential white blood cell count performed manually and using the Vision Hema® Ultimate device (p > 0.05); the results were virtually identical. The Spearman’s correlation coefficient was highest for segmented neutrophils, lymphocytes and eosinophils (0.9638%, 0.9342%, and 0.9172%, respectively). For monocytes and basophils it was 0.9047% and 0.7613%, respectively, while its lowest values were observed for immature myeloid cells. The Vision Hema® Ultimate blood smear scanner analyzer was shown to provide high accuracy and productivity of differential white blood cell count in peripheral blood smears.

About the authors

D. Yu. Sosnin

E.A.Vagner Perm State Medical University

Author for correspondence.
Email: sosnin_dm@mail.ru
Russian Federation, Perm

L. S. Onyanova

OOO West Medica

Email: sosnin_dm@mail.ru
Russian Federation, Perm

O. G. Kubarev

Perm National Research Polytechnical University

Email: sosnin_dm@mail.ru
Russian Federation, Perm

E. V. Kozonogova

Perm National Research Polytechnical University

Email: sosnin_dm@mail.ru
Russian Federation, Perm

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