The preconditioning phenomenon and its mechanisms in the common carp as affected by Actara insecticide

Abstract

The effect of Actara insecticide on the common carp (Cyprinus carpio Linnaeus) was studied, and its sublethal concentration (LC50) for this species was estimated. Exposure to Actara at a concentration of 400 mg/L in freshwater was shown to kill all the common carp individuals within 24 h, while 24-h preconditioning at an Actara concentration of 100 mg/L promoted survival of animals when they were subsequently exposed to the insecticide at a concentration of 400 mg/L for 5 days. Estimates of serotonin-modulated anticonsolidation protein (SMAP) in the common carp brain and liver by enzyme-linked immunosorbent assay (ELISA) following exposure to Actara at a concentration of 100 mg/L for 24 h showed its increased level in both organs (p < 0.001), particularly manifest in the liver. It is concluded that preconditioning elevates SMAP tissue levels and promotes thereby protection of the organism against damaging effects of lethal Actara concentrations.