X-ray diffraction analysis revealed the role of the L254 residue in the recognition of the substrate by carboxypeptidase T from Thermoactinomyces vulgaris
- 作者: Akparov V.K.1, Timofeev V.I.1, Konstantinova G.E.1, Kuranova I.P.1
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隶属关系:
- Shubnikov Institute of Crystallography of the Kurchatov Complex Crystallography and Photonics of the NRC “Kurchatov Institute”
- 期: 卷 70, 编号 4 (2025)
- 页面: 604-612
- 栏目: КРИСТАЛЛОГРАФИЯ В БИОЛОГИИ И МЕДИЦИНЕ
- URL: https://journal-vniispk.ru/0023-4761/article/view/306269
- DOI: https://doi.org/10.31857/S0023476125040099
- EDN: https://elibrary.ru/jgbdmh
- ID: 306269
如何引用文章
详细
Crystal structures of complexes of the mutant protein L254N carboxypeptidase T from Thermoactinomyces vulgaris with stable transition state analogues -N- sulfamoyl-L-glutamate, N-sulfamoyl-L-arginine, N-sulfamoyl-L-valine and N-sulfamoyl-L-leucine (resolution 2.05, 1.89, 2.30, 1.79 Å) were obtained. The dependence of the association constants of these inhibitors, as well as the efficiency of catalysis of the corresponding tripeptide substrates ZAAX, on the distances between the atoms of the ligand O15, O16, O20, T19 and the active center of the mutant protein N146, Y225 and E277 was found. This dependence differs significantly from the previously identified dependence for wild-type carboxypeptidase T. The results obtained indicate the involvement of leucine 254, which is part of the mobile loop of metallocarboxypeptidases, in the discrimination of substrates by carboxypeptidase T according to the induced fit mechanism.
作者简介
V. Akparov
Shubnikov Institute of Crystallography of the Kurchatov Complex Crystallography and Photonics of the NRC “Kurchatov Institute”
Email: valery.akparov@yandex.ru
俄罗斯联邦, Moscow, 119333
V. Timofeev
Shubnikov Institute of Crystallography of the Kurchatov Complex Crystallography and Photonics of the NRC “Kurchatov Institute”
Email: valery.akparov@yandex.ru
俄罗斯联邦, Moscow, 119333
G. Konstantinova
Shubnikov Institute of Crystallography of the Kurchatov Complex Crystallography and Photonics of the NRC “Kurchatov Institute”
Email: valery.akparov@yandex.ru
俄罗斯联邦, Moscow, 119333
I. Kuranova
Shubnikov Institute of Crystallography of the Kurchatov Complex Crystallography and Photonics of the NRC “Kurchatov Institute”
编辑信件的主要联系方式.
Email: valery.akparov@yandex.ru
俄罗斯联邦, Moscow, 119333
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