Hemoglobin-catalyzed fluorometric method for the determination of glutathione


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详细

A new spectrofluorometric method for the determination of glutathione based on the reaction catalyzed by hemoglobin was reported. The reaction product gave a highly fluorescent intensity with the excitation and emission wavelengths of 320.0 nm and 413.0 nm, respectively. The optimum experimental conditions were investigated. Results showed that low concentration glutathione enhanced the fluorescence intensity significantly. The line ranges were 1.0 × 10–6–1.0 × 10–5 mol L–1 of glutathione and 6.0 × 10-10 mol L-1–1.0 × 10-8 mol L-1, respectively. The detection limit was calculated to be 1.1 × 10-11 mol L-1. The recovery test by the standard addition method gave values in the range of 90.78%-102.20%. This method was used for the determination of glutathione in synthetic and real samples with satisfactory results.

作者简介

Ruiqiang Wang

The First Affiliated Hospital of Zhengzhou University

编辑信件的主要联系方式.
Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052

Lin Tang

The First Affiliated Hospital of Zhengzhou University

Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052

Hua Li

The First Affiliated Hospital of Zhengzhou University

Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052

Yi Wang

The First Affiliated Hospital of Zhengzhou University

Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052

Rong Gou

The First Affiliated Hospital of Zhengzhou University

Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052

Yuanyuan Guo

The First Affiliated Hospital of Zhengzhou University

Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052

Yudong Fang

The First Affiliated Hospital of Zhengzhou University

Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052

Fengmei Chen

The First Affiliated Hospital of Zhengzhou University

Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052

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