Hemoglobin-catalyzed fluorometric method for the determination of glutathione
- 作者: Wang R.1, Tang L.1, Li H.1, Wang Y.1, Gou R.1, Guo Y.1, Fang Y.1, Chen F.1
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隶属关系:
- The First Affiliated Hospital of Zhengzhou University
- 期: 卷 120, 编号 1 (2016)
- 页面: 164-170
- 栏目: Geometrical and Applied Optics
- URL: https://journal-vniispk.ru/0030-400X/article/view/164477
- DOI: https://doi.org/10.1134/S0030400X15120218
- ID: 164477
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详细
A new spectrofluorometric method for the determination of glutathione based on the reaction catalyzed by hemoglobin was reported. The reaction product gave a highly fluorescent intensity with the excitation and emission wavelengths of 320.0 nm and 413.0 nm, respectively. The optimum experimental conditions were investigated. Results showed that low concentration glutathione enhanced the fluorescence intensity significantly. The line ranges were 1.0 × 10–6–1.0 × 10–5 mol L–1 of glutathione and 6.0 × 10-10 mol L-1–1.0 × 10-8 mol L-1, respectively. The detection limit was calculated to be 1.1 × 10-11 mol L-1. The recovery test by the standard addition method gave values in the range of 90.78%-102.20%. This method was used for the determination of glutathione in synthetic and real samples with satisfactory results.
作者简介
Ruiqiang Wang
The First Affiliated Hospital of Zhengzhou University
编辑信件的主要联系方式.
Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052
Lin Tang
The First Affiliated Hospital of Zhengzhou University
Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052
Hua Li
The First Affiliated Hospital of Zhengzhou University
Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052
Yi Wang
The First Affiliated Hospital of Zhengzhou University
Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052
Rong Gou
The First Affiliated Hospital of Zhengzhou University
Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052
Yuanyuan Guo
The First Affiliated Hospital of Zhengzhou University
Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052
Yudong Fang
The First Affiliated Hospital of Zhengzhou University
Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052
Fengmei Chen
The First Affiliated Hospital of Zhengzhou University
Email: wangruiqiang@zzu.edu.cn
中国, Zhengzhou, 450052
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