Influence of combined drug tiladerm on the course of allergic contact dermatitis in rats

Materials and methods. The composition of glyzyrrhizic acid and glutamyl-tryptophane in the form of cream was studied using white rats. ACD was induced by application of the ethanol-acetone solution of 2,4-dinitrochlorobenzene (DNCB) during 4 days. The investigational drug was applied to the ACD lesions once a day during 14 consecutive days. The severity of ACD was assessed by the condition of the lesion focus, morphological composition of blood and apoptosis rate in the lesion. Results. It was shown that 4 days application of DNCB causes typical skin lesion manifesting in local papulovesicular eruptions, excoriations, and forming crust on the surface of the lesion. Application of the topical drug Tiladerm in the form of cream on the lesion induces significant acceleration of the lesion focus rehabilitation as early as on the end of 14 day of treatment. The activation of the markers of apoptosis (Ki-67, to a less extend p-53, caspase-3, Bcl-2) in the ACD lesion was shown. During treatment with Tiladerm, Ki-67 expression was reduced while expressions of p-53 and caspase-3 were enhanced. Conclusion. Thus, Tiladerm showed prominent positive effect on experimental ACD in rats.

contact dermatitis, treatment, glycyrrhizinic acid, glutamyl-tryptophan, markers of apoptosis