Sorption technology for the isolation of pancreatic enzymes from extracts of the pancreas of cattle and reindeer
- 作者: Karavaeva L.I.1, Krylova P.A.1, Moroz A.S.2, Kotova N.V.1, Glazova N.V.1, Bunyatyan N.D.3
-
隶属关系:
- St. Petersburg Chemical and Pharmaceutical University
- Peter the Great St. Petersburg Polytechnic University
- "Scientific Centre for Expert Evaluation of Medicinal Products" of the Ministry of Health of the Russian Federation
- 期: 卷 72, 编号 5 (2023)
- 页面: 39-44
- 栏目: Technology of medicines
- URL: https://journal-vniispk.ru/0367-3014/article/view/142769
- DOI: https://doi.org/10.29296/25419218-2023-05-05
- ID: 142769
如何引用文章
开放存取
##reader.subscriptionAccessGranted##
订阅存取
详细
Introduction. Pancreatin is a digestive enzyme remedy that is an extract of a pancreatic enzyme. It contains such proteolytic enzymes as amylase, lipase and protease, which are involved in the digestion of carbohydrates, fats, proteins, which contributes to their more complete absorption in the small intestine. It is used for insufficiency of the exocrine function of the pancreas (chronic pancreatitis, cystic fibrosis), for chronic inflammatory and degenerative diseases of the stomach, intestines, liver, gallbladder; conditions after resection or irradiation of these organs, accompanied by disorders of food digestion, flatulence, diarrhea (as part of combination therapy), to improve food digestion in patients with normal function of the gastrointestinal tract (GIT) in case of nutritional errors, as well as chewing disorders function, forced prolonged immobilization, sedentary lifestyle, and in preparation for x-ray and ultrasound examination of the abdominal organs.Thus, pancreatin is one of the most popular medical preparations and is produced under various trade names. However, pancreatin is not produced in Russia and is supplied to the country mainly from China and Germany.
Objective: Development of a technology for the isolation of pacreatic enzymes from extracts of the semigastric glands of SO and cattle.
Material and methods. In the work used – the pancreas from the reindeer. The determination of the component composition was carried out by the methods of gel chromatography.
Results. Methods have been developed for isolating a complex of digestive enzymes from the pancreas of CO and cattle.
Conclusion. The optimal conditions for the process of BAS extraction from the pancreas of CO and the pancreas of cattle have been selected. The component composition of the obtained extracts was determined.The optimization of the sorption technology for the isolation of enzymes from extracts of cattle and CO was developed and carried out.
作者简介
Lolita Karavaeva
St. Petersburg Chemical and Pharmaceutical University
编辑信件的主要联系方式.
Email: lolita.karavaeva@spcpu.ru
ORCID iD: 0000-0001-8960-4617
Post-graduate student of the Department of Biotechnology
俄罗斯联邦, Professor Popov str., 14, Sant-Petersburg, 197022Polina Krylova
St. Petersburg Chemical and Pharmaceutical University
Email: polina.krylova@spcpu.ru
ORCID iD: 0000-0003-2532-248X
student of the Department of Biotechnology
俄罗斯联邦, Professor Popov str., 14, Sant-Petersburg, 197022Anna Moroz
Peter the Great St. Petersburg Polytechnic University
Email: anna.kurochkina@pharminnotech.com
ORCID iD: 0009-0003-9536-7058
Assistant of the Department of Biotechnology
俄罗斯联邦, Politechnicheskaya str., 29, Saint-Petersburg, 195251Natalia Kotova
St. Petersburg Chemical and Pharmaceutical University
Email: Natalia.kotova@pharminnotech.com
ORCID iD: 0000-0001-5483-6717
Associate Professor, Department of Biotechnology, Candidate of Chemical Sciences
俄罗斯联邦, Professor Popov str., 14, Sant-Petersburg, 197022Natalya Glazova
St. Petersburg Chemical and Pharmaceutical University
Email: Natalia.glazova@pharminnotech.com
ORCID iD: 0000-0003-3242-3051
Associate Professor of the Department of Biotechnology, Candidate of Chemical Sciences
俄罗斯联邦, Professor Popov str., 14, Sant-Petersburg, 197022Natalia Bunyatyan
"Scientific Centre for Expert Evaluation of Medicinal Products" of the Ministry of Health of the Russian Federation
Email: Bunyatyan@expmed.ru
ORCID iD: 0000-0003-0936-5551
Chief Analyst of the Center for Clinical Pharmacology, Doctor of Pharmaceutical Sciences, Professor
俄罗斯联邦, Petrovsky boulevard, 8/2, Moscow, 127051参考
- Karavaeva L.I., Kotova N.V., Glazova N.V., Bunyatyan N.D., Evteev V.A., Prokofiev A.B. Modern approaches to the isolation and purification of active biotechnological substances (ABTS) from reindeer organs. Scientific and practical J. Pharmacy. 2022; 5: 33–7. doi: 10.29296/25419218-2022-05-05 (in Russian)
- Determination of protein concentration in solution (according to Lowry O.H.). [Electronic resource]. Access mode: https://www.sciencedirect.com/science/article/pii/S0021925819524516 (accessed 03/19/2023) (in Russian)
- Determination of protease activity (according to M. Kunitz). [Electronic resource]. Access mode: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2141215/pdf/313.pdf. (date of access: 03/19/2023) (in Russian)
- Determination of lipase activity (according to Shihabi and Bishop). [Electronic resource]. Access mode: http://neznaniya.net/medicina/ostryj-pankreatit/2326-opredelenie-aktivnosti-lipazy-po-shihabi-i-bishop.html (date of access: 03/19/2023) (in Russian)
- Rukhlyadeva A.P., Polygalina T.V. Methods for determining the activity of hydrolytic enzymes. M.: Light and food pr-t, 1981; 288 (in Russian)
- Pisarev O.A., Glazova N.V. Application of new methods of preparative low-pressure chromatography to improve the quality of drugs. Sorption and chromatographic processes. 2001; 1 (2): 415–23 (in Russian)
- Karavaeva L.I., Stuchaeva A.A., Glazova N.V. Modification of hydrolytic enzymes from the pancreas of a reindeer in order to develop a sorption-chromatographic method for isolation and purification. International Forum Biotechnology: state and development prospects. Moscow, 2020; 148–50 (in Russian)
- Glazova N.V., Kucherenko (Serkova) A.N., Omelyanov A.P. Modern technologies for isolation, purification and modification of biotechnological APIs (enzymes): monograph. Moscow: KNORUS, 2019; 152 (Monograph) (in Russian)
- Karavaeva L.I., Glazova N.V. Selection of sorbents for the development of a sorption method for the isolation and purification of a complex of hydrolytic enzymes from the pancreas of a reindeer. Collection of materials of the X All-Russian Scientific Conference of Students and Postgraduates with international participation conf. "Young Pharmacy – Potential for the Future", April 20–21. St. Petersburg: SPKhFU, 2020; 684–6 (in Russian)
- Karavaeva L.I., Glazova N.V. Stabilization of hydrolytic enzymes from the pancreas of a reindeer in order to develop a sorption-chromatographic method for isolation and purification. I International Scientific and Practical Internet Conference "Achievements and prospects of science, education and production: 2020". Kiev, Ukraine, 461–8 (in Russian)
补充文件
