Investigation of flavonoids of the herb alfalfa sickle (Medicago falcata L.)
- Authors: Shvets N.N.1, Bubenchikova V.N.1
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Affiliations:
- FSBEI HE KSMU Ministry of Health
- Issue: Vol 71, No 8 (2022)
- Pages: 15-20
- Section: Pharmaceutical chemistry and pharmacognosy
- URL: https://journal-vniispk.ru/0367-3014/article/view/144030
- DOI: https://doi.org/10.29296/25419218-2022-08-02
- ID: 144030
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Abstract
Introduction. Crescent alfalfa (Medicago falcata L.) is widespread in the regions of central Russia, it is a fodder and honey plant. The plant contains various classes of biologically active substances, among which flavonoids, isoflavonoids are more valuable, because. have different pharmacological efficacy, such as anti-inflammatory, anticarcinogenic, antimicrobial, antioxidant.
In this regard, the identification and qualitative determination of individual flavonoids of the herb sickle alfalfa by various methods is relevant.
Objective: study of the flavonoid compounds of the alfalfa herb by known qualitative reactions, paper chromatography and ultra performance liquid chromatography/MS.
Material and methods. The flavonoid composition of sickle alfalfa was studied using qualitative reactions, paper chromatography, ultra performance liquid chromatography/MS on a Waters Acquility chromatograph with a UV diode array detector and a TQD tandem quadrupole MS detector (Waters) in the gradient elution mode. Flavonoid compounds were extracted with 70% ethyl alcohol.
Results. The flavonoid composition of the crescent alfalfa herb is represented by derivatives of quercetin, myricetin, apigenin, mainly their methoxylated forms. A total of 10 compounds were identified, including 8 flavonols, which were divided into quercetin derivatives (rutin, 4',7-O-Dimethylquercetin-3-O-di-methylglucoside, 4',7-O-dimethylquercetin-3-O-methylglucoside) , the rest are classified as myricetin derivatives. Two compounds are classified as flavones (apigenin-7-O-methylglucoside, apigenin-7-O-acetylglucosyl, glucosylglucoside).
Conclusion. 10 flavonoid compounds, represented mainly by methoxylated compounds, were identified in the herb alfalfa sickle, and therefore it can serve as a potential source of these compounds.
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##article.viewOnOriginalSite##About the authors
Natalya Nikolaevna Shvets
FSBEI HE KSMU Ministry of Health
Author for correspondence.
Email: nataliashvetzneo@mail.com
ORCID iD: 0000-0003-4550-781X
postgraduate student of the Department of Pharmacognosy and Botany, Federal State Budgetary Educational Institution of Higher Education "Kursk State Medical University"
St. K. Marx, h. 3, Kursk, Kursk region, 305041, Russian FederationValentina Nikolaevna Bubenchikova
FSBEI HE KSMU Ministry of Health
Email: bubenhikova.ksmu@yandex.ru
ORCID iD: 0000-0001-9682-0684
Doctor of Pharmacy, Professor, Head of the Department of Pharmacognosy and Botany, Federal State Budgetary Educational Institution of Higher Education "Kursk State Medical University"
St. K. Marx, h. 3, Kursk, Kursk region, 305041, Russian FederationReferences
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