De Novo Transcriptome Sequencing and Analysis to Identify Genes Involved in the Biosynthesis of Flavonoids in Abrus mollis Leaves

Abstract

Abrus mollis Hance is an endemic genuine medicinal material growing widely in Southern China. We used the next-generation sequencing technology to carry out de novo transcriptome sequencing and analysis of A. mollis leaves. 57669118 high quality reads and 53743 assembled unigenes were obtained. 36271 unigenes (67.49%) were annotated in the four public databases (NCBI-nr, Swiss-Prot, KEGG, and KOG). After comprehensive analysis of the A. mollis transcriptome, 35656 unigenes were classified into 25 KOG function classifications and 7572 unigenes were assigned to 128 different KEGG pathways. Then, the unigenes with their key enzymes in phenylalanine, phenylpropanoid, flavonoids and isoflavonoid biosynthesis pathway were investigated in KEGG pathways. The study results will be helpful to reveal the biosynthesis mechanism of flavonoids and isoflavonoid in A. mollis leaves and carry out its biological regulation research.