STEARYLAMINE ACTIVATES RAPID AND ROS-INDEPENDENT FORMATION OF NEUTROPHIL EXTRACELLULAR TRAPS (NETOSIS)

Using confocal fluorescence microscopy it was shown that stearylamine (SA) at concentration of 0.2 mg/ml causes the neutrophil extracellular traps formation. In comparison with classic NET activator, the phorbol ether of myristate acetate (PMA), SA activates this process much faster, in 30‒60 min, while under PMA stimulation it occurs in 2‒3 hours. Using luminol-dependent chemiluminescence, we showed that SA does not activate NADPH oxidase. SA dose-dependently suppresses the oxidative burst stimulated with PMA, zymosan, or latex. The direct evidence that NADPH oxidase is not activated under the SA action was obtained by using neutrophils isolated from the blood of patients with chronic granulomatous disease (CGD). It is obvious from our results, that SA-induced NET formation does not require the participation of reactive oxygen species and proceeds according to a nonclassical mechanism.

fluorescence confocal microscopy, luminol-dependent chemiluminescence, NETosis, neutrophil extracellular traps, stearylamine