Vol 22, No 2-2 (2019)

It has been established that during endometriosis, regardless of the severity of the disease, at the systemiс level IL-4 synthesis, intracellular expression of IL-4 and IL-6 were increased in the total lymphocyte and in the T-helper population, and in the peritoneal fluid the level of IL-6⁺ lymphocytes was increased. Changes in the synthesis, intracellular expression and the serum IL-4 content were proportional to the severity of endometriosis. In endometriosis stages I–II the intracellular production of IL-5 increased in the total lymphocyte population and in the T-helper pool. In endometriosis III–IV the IL-6 synthesis by peripheral lymphocytes increased, as well as the serum level of IL-4, the amount of IL-4⁺, CD4⁺IL-4⁺ lymphocytes and sCD30 in the peritoneal fluid. 

The study involved healthy women, the average age of whom was 45.0±3.41. All patients received a single procedure for the biorevitalization of the facial skin. Some indicators of systemic and tissue immunity were evaluated. When analyzing the number of immune cells in the skin before and after the procedure, the amount of CD3⁺, CD4⁺, CD8⁺ was doubled. The amount of CD20+ remained practically unchanged. When assessing immunoglobulins M, G in the skin before and after the procedure, a decrease in these indicators was observed. IgA practically did not change. The amount of immunoglobulins in the skin changed as well as in the blood. It decreased. When assessing the parameters of cellular immunity of blood, it was found out that the cellular composition of the blood does not practically change. Significant changes occurred only in the absolute and relative amount of CD3^–CD56⁺. These indicators signifi cantly increased.  

Modern methods of multicolor flow cytometry in combination with MHC multimer technologies allow not only to identify and isolate cytotoxic T-lymphocyte (CTL) populations, but also to conduct phenotypic research on the level of differentiation, functional features of these cells, and even to investigate the distribution of memory T-cell subsets inside populations specific to particular epitopes of tumor antigens. In the present study, we assessed the level of differentiation and the content of memory T-cell subpopulations in cytotoxic T-lymphocyte populations specific for HER2/neu antigen epitopes (HER2) using the MHC-multimers antigen-specific CD8⁺ T-cell staining method and multicolor flow cytometry. The resulting HER2-specific T cells to a large extent (about 40‒50%) have the TSCM phenotype, a population capable of the most pronounced antitumor immune response due to a combination of effector properties with self-sustainability. 

Allogeneic biomaterials (AB) after implantation in a defect in the calf muscles of rats and the uterine horn of the rat contribute to the regeneration of tissues due to migration of M1 macrophages expressing Pro-inflammatory factors, TNFα, IL-1. They inhibit the migration of M2 macrophages, fibrosis factors, promote migration and differentiation of myocytes. IN ischemic damaged myocardium after AB implantation, on the contrary, cd68 macrophage deficiency was found, but high expression by Timp2 cells compared to the control, which could contribute to the reduction of scar area. After the introduction of AB in muscle tissues revealed resident macrophages with phenotype IL-1⁺/CD68⁺/PCNA⁺/Hale⁺/ vimentin⁺/FGF1^- /TGF-β1^–. 

A model of the bacterial process under the influence of fMLP was reproduced in the in vitro system, which made it possible to reveal the features of the transformation of CD66b⁺CD33⁺CD16⁺CD11b⁺neutrofi l granulocytes (NG). Multidirectional effects of mono-effects of peptides and rIFNα2b on the expression of the studied markers of NG in healthy children were identified. The modulating effect of rIFNα2b on the transformed fMLP phenotype of NG is associated with polarization into an anti-inflammatory phenotype. 

The content of endothelial progenitor cells in umbilical cord blood was determined in newborns born to mothers with preeclampsia and without preeclampsia. Phenotyping of umbilical cord blood endothelial cells was performed by flow cytometry, circulating endothelial cells (CES) were defined as: CD45^–, CD133⁺, VEGFR2⁺, CD34^–, endothelial progenitor cells (EPCS) were defined as: CD45^–, CD133⁺, VEGFR2⁺, CD34⁺. There was a decrease in both the total number of circulating endothelial cells and endothelial progenitor cells in premature infants compared to full-term ones. In newborns born to mothers with preeclampsia, only the number of ECP was significantly higher, compared with newborns from mothers without preeclampsia, and in the group of premature and full-term newborns. 

In the present work we demonstrated that IFNα-induced dendritic cells (IFN-DCs) loaded with a lysate of autologous tumor cells of high-grade glioma patients (DC_LYS) more eff ectively stimulated the proliferative response of autologous T-cells compared with unloaded IFN-DCs (DC_LPS) and did not induce apoptosis of CD4⁺ and CD8⁺ T cells. Moreover, DC_LYS activated cytotoxic CD8⁺ T cells (in the degranulation test) in cultures of autologous mononuclear cells of studied patients. 

The patients with a diagnosis of acute non-obstructive (ANOP) and obstructive pyelonephritis (AOP) (31 and 30 patients respectively) were under observation during treatment at the urological center of the National Healthcare Institution Scientifi c Clinical Center of Russian Railways. The levels of cytokines, complement components and their inhibitors in plasma and the first portion of morning urine, phagocytic and oxygen-dependent activity of circulating blood neutrophils were determined. At the systemic level, in patients with ANOP, to a greater extent in AOP, an increase in the concentration of pro-inflammatory cytokines (TNFα, IL-6, IL-8 and IFNγ), IFNα, IL-4 and IL-10, components of the C₃ and C_5a compliment was established, a decrease in the level of IL-1RA and a regulator of the complement factor H system, a decrease in the activity and intensity of phagocytosis of peripheral blood neutrophils with an increase in the activity of oxygen-dependent systems were also revealed. Before treatment at the local level (in the urine) in patients with ANOP, an increase in the concentration of all studied cytokines, except IL-4, components C₃ and C₅, and a decrease in IL-1RA was established. In AOP, changes were more pronounced, with the exception of a reduced level of factor H and sIgA. 

The lack of effective vaccines against Ebola virus initiates a search for new approaches to solving this problem. One of the promising directions in development of antiviral vaccines based on designing DNA-vaccines encoding artificial polyepitope immunogens. Previously, using the original software, we designed two polyepitopic T-cell immunogens containing cytotoxic and T-helper epitopes of Ebola virus and showed that DNA vaccines based on them provide synthesis of target mRNA and proteins in a culture of eukaryotic cells. As part of this work, we have shown that engineered DNA vaccine constructs induce virus-specific responses of CD4⁺ and CD8⁺ T-lymphocytes in immunized mice. 

Associations between polymorphic sites rs2239815 of gene MAPK8, rs1053023 of gene STAT3, rs4143094 of gene GATA3, rs28362491 of gene NF-kB1 with the absolute and relative number of CD19+ lymphocytes, CD3⁺CD4⁺ lymphocytes and with the absolute content of CD3⁺ and CD3⁺CD8⁺ lymphocytes were registeredin exposed persons.