PHENOTYPING OF ANTIGEN-SPECIFIC CYTOTOXIC T-LYMPHOCYTES BY THE METHOD OF MULTICOLOR FLOW CYTOMETRY
- Authors: Kuznetsova M.S.1, Lopatnikova J.A.1, Sennikov S.V.1,2
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Affiliations:
- Research Institute of Fundamental and Clinical Immunology
- Novosibirsk State University
- Issue: Vol 22, No 2-2 (2019)
- Pages: 837-839
- Section: ORIGINAL ARTICLES
- URL: https://journal-vniispk.ru/1028-7221/article/view/119709
- DOI: https://doi.org/10.31857/S102872210006680-3
- ID: 119709
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Abstract
Modern methods of multicolor flow cytometry in combination with MHC multimer technologies allow not only to identify and isolate cytotoxic T-lymphocyte (CTL) populations, but also to conduct phenotypic research on the level of differentiation, functional features of these cells, and even to investigate the distribution of memory T-cell subsets inside populations specific to particular epitopes of tumor antigens. In the present study, we assessed the level of differentiation and the content of memory T-cell subpopulations in cytotoxic T-lymphocyte populations specific for HER2/neu antigen epitopes (HER2) using the MHC-multimers antigen-specific CD8+ T-cell staining method and multicolor flow cytometry. The resulting HER2-specific T cells to a large extent (about 40‒50%) have the TSCM phenotype, a population capable of the most pronounced antitumor immune response due to a combination of effector properties with self-sustainability.
About the authors
M. S. Kuznetsova
Research Institute of Fundamental and Clinical Immunology
Author for correspondence.
Email: fake@neicon.ru
junior researcher of the Laboratory of Molecular Immunology,
Novosibirsk
Russian FederationJ. A. Lopatnikova
Research Institute of Fundamental and Clinical Immunology
Email: fake@neicon.ru
PhD, senior researcher of the Laboratory of Molecular Immunology,
Novosibirsk
Russian FederationS. V. Sennikov
Research Institute of Fundamental and Clinical Immunology;Novosibirsk State University
Email: sennikovsv@gmail.com
PhD, MD, professor, head of the Laboratory of Molecular Immunology,
Novosibirsk
Russian FederationReferences
- Mahnke Y. D., Brodie T. M., Sallusto F., Roederer M., Lugli E. The who’s who of T-cell differentiation: Human memory T-cell subsets // Eur J Immunol 2013, 43, 2797–2809.
- Кудрявцев И. В. Т-клетки памяти: основные популяции и стадии дифференцировки // Российский иммунологический журнал 2014, 8(17), 4, 947– 964.
- Хайдуков С. В., Зурочка А. В., Тотолян А. А., Чере шнев В. А. Основные и малые популяции лимфоцитов периферической крови человека и их нормативные значения (методом многоцветного цитометрического анализа) // Медицинская Иммунология 2009, 11, 2–3, 227–238.
- Kuznetsova M., Lopatnikova J., Khantakova J., Maksyutov R., Maksyutov A., Sennikov S. Generation of populations of antigen-specific cytotoxic T cells using DCs transfected with DNA construct encoding HER2/neu tumor antigen epitopes. // BMC Immunol 2017, 18, doi: 10.1186/s12865-017-0219-7.
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