Отправить статью по E-mail Microfluidic chips for the study of cell migration under the effect of chemicals
- Авторы: Kukhtevich I.V.1,2, Belousov K.I.1, Bukatin A.S.1, Chubinskiy-Nadezhdin V.I.3, Vasileva V.Y.3, Negulyaev Y.A.3,4, Evstrapov A.A.1,2,5
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Учреждения:
- Institute for Analytical Instrumentation
- National Research University of Information Technologies, Mechanics, and Optics (ITMO University)
- Institute of Cytology
- Peter the Great Polytechnic University
- St. Petersburg Academic University
- Выпуск: Том 42, № 5 (2016)
- Страницы: 478-481
- Раздел: Article
- URL: https://journal-vniispk.ru/1063-7850/article/view/199016
- DOI: https://doi.org/10.1134/S1063785016050102
- ID: 199016
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Аннотация
Numerical simulation of the formation of a chemoattractant gradient in reaction chambers of a chip having different geometries enabled the determination of a structure suitable for the study of cell migration, in accordance with which hybrid polymer–glass microfluidic devices were manufactured. Verification of the procedures of alignment of cells in the reaction chamber of the chip by centrifugal force and subsequent culturing of the cells showed that microfluidic chips can be used to study cell migration under the effect of the chemoattractant gradient in vitro.
Об авторах
I. Kukhtevich
Institute for Analytical Instrumentation; National Research University of Information Technologies, Mechanics, and Optics (ITMO University)
Email: ba@inbox.ru
Россия, St. Petersburg, 198095; St. Petersburg, 197101
K. Belousov
Institute for Analytical Instrumentation
Email: ba@inbox.ru
Россия, St. Petersburg, 198095
A. Bukatin
Institute for Analytical Instrumentation
Автор, ответственный за переписку.
Email: ba@inbox.ru
Россия, St. Petersburg, 198095
V. Chubinskiy-Nadezhdin
Institute of Cytology
Email: ba@inbox.ru
Россия, St. Petersburg, 194064
V. Vasileva
Institute of Cytology
Email: ba@inbox.ru
Россия, St. Petersburg, 194064
Yu. Negulyaev
Institute of Cytology; Peter the Great Polytechnic University
Email: ba@inbox.ru
Россия, St. Petersburg, 194064; St. Petersburg, 195251
A. Evstrapov
Institute for Analytical Instrumentation; National Research University of Information Technologies, Mechanics, and Optics (ITMO University); St. Petersburg Academic University
Email: ba@inbox.ru
Россия, St. Petersburg, 198095; St. Petersburg, 197101; St. Petersburg, 194021
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