Preparation of the amphiphilic copolymer of N-vinylpyrrolidone with triethylene glycol dimethacrylate nanoparticles and the study of their properties in vitro

Abstract

According to DLS the amphiphilic copolymer of N-vinylpyrrolidone with triethylene glycol dimethacrylate of branched topology forms micelle-like particles in isopropyl alcohol with the hydrodynamic radius R_h of ~50 nm. Due to the solubilization of hydrophobic zinc tetra-phenylporphyrinate by polymer particles, the water soluble macromolecular hybrid structures of R_h ~20 nm were obtained. The structures are stable in aqueous buffer solution at pH 6.8—7.0 and demonstrate characteristic fluorescence. According to TEM, the obtained nanoparticles have close to spherical shape. They exhibit low toxicity for cells at the concentration below 700 μg mL^–1. As it is shown by fluorescence microscopy, the polymer particles with encapsulated dye are able to penetrate into the cells.