Chemically induced dynamic nuclear polarization study of the reduction of histidine radical in the reactions with aromatic amino acids
- Авторы: Morozova O.B.1,2, Fishman N.N.1,2, Yurkovskaya A.V.1,2
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Учреждения:
- International Tomography Center, Siberian Branch of Russian Academy of Sciences
- Novosibirsk State University
- Выпуск: Том 65, № 12 (2016)
- Страницы: 2907-2913
- Раздел: Full Articles
- URL: https://journal-vniispk.ru/1066-5285/article/view/239605
- DOI: https://doi.org/10.1007/s11172-016-1676-5
- ID: 239605
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Аннотация
In order to model the involvement of histidine radicals in the electron transfer reactions in proteins, we investigated the kinetics of the reduction of hisitidine radicals in the reactions with aromatic amino acid residues by means of chemically induced dynamic nuclear polarization with microsecond time resolution. Histidine radicals were generated in photochemical reaction with triplet-excited 3,3’,4,4’-benzophenone tetracarboxylic acid. The intermolecular electron transfer from N-acetyl derivatives of tyrosine and tryptophan to the radical of N-acetyl histidine was studied in neutral and basic aqueous solutions. It was found that the rate constant of reduction of short-lived histidine radical in the reactions with tyrosine anion is kr = (1.9±0.5)•108 L mol–1 s–1, which is more than two orders of magnitude higher than that of reduction of histidine radical with neutral tyrosine (kr = (1.2±0.4)•106 L mol–1 s–1). In the reduction of histidine radical with N-acetyl tryptophan, the protonated states of reagents do not change, when pH of aqueous solution varies from neutral to basic and the reaction rate coefficient remains constant being kr = (1.3±0.4)•106 L mol–1 s–1.
Об авторах
O. Morozova
International Tomography Center, Siberian Branch of Russian Academy of Sciences; Novosibirsk State University
Email: yurk@tomo.nsc.ru
Россия, 3a ul. Institutskaya, Novosibirsk, 630090; 2 ul. Pirogova, Novosibirsk, 630090
N. Fishman
International Tomography Center, Siberian Branch of Russian Academy of Sciences; Novosibirsk State University
Email: yurk@tomo.nsc.ru
Россия, 3a ul. Institutskaya, Novosibirsk, 630090; 2 ul. Pirogova, Novosibirsk, 630090
A. Yurkovskaya
International Tomography Center, Siberian Branch of Russian Academy of Sciences; Novosibirsk State University
Автор, ответственный за переписку.
Email: yurk@tomo.nsc.ru
Россия, 3a ul. Institutskaya, Novosibirsk, 630090; 2 ul. Pirogova, Novosibirsk, 630090
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