New monoclonal antibodies to the Ebola virus glycoprotein: Identification and analysis of the amino acid sequence of the variable domains
- Authors: Panina A.A.1, Aliev T.K.2, Shemchukova O.B.3, Dement’yeva I.G.3, Varlamov N.E.3, Pozdnyakova L.P.3, Bokov M.N.3, Dolgikh D.A.1,4, Sveshnikov P.G.3, Kirpichnikov M.P.1,4
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Faculty of Chemistry
- All-Russia Research Center of Molecular Diagnostics and Therapy
- Faculty of Biology
- Issue: Vol 467, No 1 (2016)
- Pages: 117-120
- Section: Biochemistry, Biophysics and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/211050
- DOI: https://doi.org/10.1134/S1607672916020113
- ID: 211050
Cite item
Abstract
We determined the nucleotide and amino acid sequences of variable domains of three new monoclonal antibodies to the glycoprotein of Ebola virus capsid. The framework and hypervariable regions of immunoglobulin heavy and light chains were identified. The primary structures were confirmed using massspectrometry analysis. Immunoglobulin database search showed the uniqueness of the sequences obtained.
About the authors
A. A. Panina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Author for correspondence.
Email: panina@mx.ibch.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997
T. K. Aliev
Faculty of Chemistry
Email: panina@mx.ibch.ru
Russian Federation, Moscow, 119991
O. B. Shemchukova
All-Russia Research Center of Molecular Diagnostics and Therapy
Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149
I. G. Dement’yeva
All-Russia Research Center of Molecular Diagnostics and Therapy
Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149
N. E. Varlamov
All-Russia Research Center of Molecular Diagnostics and Therapy
Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149
L. P. Pozdnyakova
All-Russia Research Center of Molecular Diagnostics and Therapy
Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149
M. N. Bokov
All-Russia Research Center of Molecular Diagnostics and Therapy
Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149
D. A. Dolgikh
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Faculty of Biology
Email: panina@mx.ibch.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997; Moscow, 119991
P. G. Sveshnikov
All-Russia Research Center of Molecular Diagnostics and Therapy
Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149
M. P. Kirpichnikov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Faculty of Biology
Email: panina@mx.ibch.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997; Moscow, 119991
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