New monoclonal antibodies to the Ebola virus glycoprotein: Identification and analysis of the amino acid sequence of the variable domains


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Abstract

We determined the nucleotide and amino acid sequences of variable domains of three new monoclonal antibodies to the glycoprotein of Ebola virus capsid. The framework and hypervariable regions of immunoglobulin heavy and light chains were identified. The primary structures were confirmed using massspectrometry analysis. Immunoglobulin database search showed the uniqueness of the sequences obtained.

About the authors

A. A. Panina

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry

Author for correspondence.
Email: panina@mx.ibch.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997

T. K. Aliev

Faculty of Chemistry

Email: panina@mx.ibch.ru
Russian Federation, Moscow, 119991

O. B. Shemchukova

All-Russia Research Center of Molecular Diagnostics and Therapy

Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149

I. G. Dement’yeva

All-Russia Research Center of Molecular Diagnostics and Therapy

Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149

N. E. Varlamov

All-Russia Research Center of Molecular Diagnostics and Therapy

Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149

L. P. Pozdnyakova

All-Russia Research Center of Molecular Diagnostics and Therapy

Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149

M. N. Bokov

All-Russia Research Center of Molecular Diagnostics and Therapy

Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149

D. A. Dolgikh

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Faculty of Biology

Email: panina@mx.ibch.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997; Moscow, 119991

P. G. Sveshnikov

All-Russia Research Center of Molecular Diagnostics and Therapy

Email: panina@mx.ibch.ru
Russian Federation, Simferopol’skii bul’var 8, Moscow, 117149

M. P. Kirpichnikov

Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Faculty of Biology

Email: panina@mx.ibch.ru
Russian Federation, ul. Miklukho-Maklaya 16/10, Moscow, 117997; Moscow, 119991

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