Email this article Expression and purification of an engineered human endothelin receptor B in a monomeric form
- Authors: Mishin A.V.1, Luginina A.P.1, Potapenko A.P.1, Borshchevskiy V.I.1,2, Katritch V.3, Edelweiss E.4,5, Okhrimenko I.S.1, Gordeliy V.I.1,5,6,7,2, Cherezov V.G.1,3
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Affiliations:
- Moscow Institute of Physics and Technology (State University)
- Institute of Complex Systems (ICS), ICS-6: Molecular Biophysics
- Bridge Institute, Department of Chemistry
- The European Molecular Biology Laboratory (EMBL)
- CNRS
- Universite Grenoble Alpes
- CEA
- Issue: Vol 467, No 1 (2016)
- Pages: 157-161
- Section: Biochemistry, Biophysics and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/211153
- DOI: https://doi.org/10.1134/S1607672916020216
- ID: 211153
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Abstract
In humans, two endothelin receptors, ETa and ETb, are activated by three endogenous 21-mer cyclic peptides, ET-1, ET-2, and ET-3, which control various physiological processes, including vasoconstriction, vasodilation, and stimulation of cell proliferation. The first stage of this study it to produce a stable solubilized and purified receptor in a monodisperse state. This article is focused on the engineering, expression, purification, and characterization of the endothelin receptor B for subsequent structural and functional studies.
About the authors
A. V. Mishin
Moscow Institute of Physics and Technology (State University)
Author for correspondence.
Email: mishinalexej@gmal.com
Russian Federation, Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700
A. P. Luginina
Moscow Institute of Physics and Technology (State University)
Email: mishinalexej@gmal.com
Russian Federation, Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700
A. P. Potapenko
Moscow Institute of Physics and Technology (State University)
Email: mishinalexej@gmal.com
Russian Federation, Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700
V. I. Borshchevskiy
Moscow Institute of Physics and Technology (State University); Institute of Complex Systems (ICS), ICS-6: Molecular Biophysics
Email: mishinalexej@gmal.com
Russian Federation, Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700; Juelich, 52425
V. Katritch
Bridge Institute, Department of Chemistry
Email: mishinalexej@gmal.com
United States, Los Angeles, California, 90089
E. Edelweiss
The European Molecular Biology Laboratory (EMBL); CNRS
Email: mishinalexej@gmal.com
France, Grenoble Cedex 9, 38042; Grenoble, 38044
I. S. Okhrimenko
Moscow Institute of Physics and Technology (State University)
Email: mishinalexej@gmal.com
Russian Federation, Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700
V. I. Gordeliy
Moscow Institute of Physics and Technology (State University); CNRS; Universite Grenoble Alpes; CEA; Institute of Complex Systems (ICS), ICS-6: Molecular Biophysics
Email: mishinalexej@gmal.com
Russian Federation, Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700; Grenoble, 38044; Grenoble, 38044; Grenoble, 38044; Juelich, 52425
V. G. Cherezov
Moscow Institute of Physics and Technology (State University); Bridge Institute, Department of Chemistry
Email: mishinalexej@gmal.com
Russian Federation, Institutskii per. 9, Dolgoprudnyi, Moscow oblast, 141700; Los Angeles, California, 90089
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