Email this article Comparative characteristics of two anion-channel rhodopsins and prospects of their use in optogenetics
- Authors: Dolgikh D.A.1,2,3, Malyshev A.Y.4, Roshchin M.V.4, Smirnova G.R.4, Nekrasova O.V.2,3, Petrovskaya L.E.1,3, Feldman T.B.2,5, Balaban P.M.4, Kirpichnikov M.P.2,3, Ostrovsky M.A.2,5
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Affiliations:
- Pirogov Russian National Research Medical University
- Biological Faculty
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Institute of Higher Nervous Activity and Neurophysiology
- Emanuel Institute of Biochemical Physics
- Issue: Vol 471, No 1 (2016)
- Pages: 440-442
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/211778
- DOI: https://doi.org/10.1134/S160767291606017X
- ID: 211778
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Abstract
Anion-selective opsins slow ChloC and ACR2 were expressed in rat brain cortical neurons by electroporation in utero. It is shown that the light-activated channel ACR2 has pronounced advantages in terms of both the inactivation kinetics and the neuron inhibition intensity, which is associated with a more negative value of the light-activated current reversal potential compared to the slow ChloC channel. The identified properties of opsin ACR2 indicate that it can be used for strictly controlled suppression of neuronal activity in optogenetic experiments, including the expression in the retinal ganglionic cells for reconstituting the OFF-component of their receptive field, which is essential for optogenetic prosthetics of degenerative retina.
About the authors
D. A. Dolgikh
Pirogov Russian National Research Medical University; Biological Faculty; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 117997; Moscow, 119991; Moscow, 117997
A. Yu. Malyshev
Institute of Higher Nervous Activity and Neurophysiology
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 11748
M. V. Roshchin
Institute of Higher Nervous Activity and Neurophysiology
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 11748
G. R. Smirnova
Institute of Higher Nervous Activity and Neurophysiology
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 11748
O. V. Nekrasova
Biological Faculty; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 119991; Moscow, 117997
L. E. Petrovskaya
Pirogov Russian National Research Medical University; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 117997; Moscow, 117997
T. B. Feldman
Biological Faculty; Emanuel Institute of Biochemical Physics
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 119991; Moscow, 117977
P. M. Balaban
Institute of Higher Nervous Activity and Neurophysiology
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 11748
M. P. Kirpichnikov
Biological Faculty; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 119991; Moscow, 117997
M. A. Ostrovsky
Biological Faculty; Emanuel Institute of Biochemical Physics
Author for correspondence.
Email: ostrovsky3535@mail.ru
Russian Federation, Moscow, 119991; Moscow, 117977
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