Email this article The First Recombinant Viper Three-Finger Toxins: Inhibition of Muscle and Neuronal Nicotinic Acetylcholine Receptors
- Authors: Makarova Y.V.1, Kryukova E.V.1, Shelukhina I.V.1, Lebedev D.S.1, Andreeva T.V.1, Ryazantsev D.Y.1, Balandin S.V.1, Ovchinnikova T.V.1,2, Tsetlin V.I.1, Utkin Y.N.1
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Sechenov First Moscow State Medical University
- Issue: Vol 479, No 1 (2018)
- Pages: 127-130
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/212248
- DOI: https://doi.org/10.1134/S1607672918020205
- ID: 212248
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Abstract
Genes encoding two three-finger toxins TFT-AF and TFT-VN, nucleotide sequences of which were earlier determined by cloning cDNA from venom glands of vipers Azemiops feae and Vipera nikolskii, respectively, were expressed for the first time in E. coli cells. The biological activity of these toxins was studied by electrophysiological techniques, calcium imaging, and radioligand analysis. It was shown for the first time that viper three-finger toxins are antagonists of nicotinic acetylcholine receptors of neuronal and muscle type.
About the authors
Ya. V. Makarova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Author for correspondence.
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997
E. V. Kryukova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997
I. V. Shelukhina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997
D. S. Lebedev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997
T. V. Andreeva
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997
D. Yu. Ryazantsev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997
S. V. Balandin
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997
T. V. Ovchinnikova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Sechenov First Moscow State Medical University
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997; Moscow, 119992
V. I. Tsetlin
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997
Yu. N. Utkin
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: yanson1@yandex.ru
Russian Federation, Moscow, 117997
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