Email this article The Application of Recombinant Phototoxins 4D5scFv-miniSOG and DARPin-miniSOG to Study the HER2 Receptor Internalization
- Authors: Kuzichkina E.O.1,2, Shilova O.N.1, Deyev S.M.1, Petrov R.V.1
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Affiliations:
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
- Moscow Institute of Physics and Technology (State University)
- Issue: Vol 482, No 1 (2018)
- Pages: 245-248
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/212435
- DOI: https://doi.org/10.1134/S1607672918050046
- ID: 212435
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Abstract
It was found that the fluorescent properties of the phototoxic domain of miniSOG allow to assess the ability of toxins to bind to human breast adenocarcinoma cells SK-BR-3 and study the dynamics of their internalization. We established that the main cause of the decrease of the fluorescence intensity of the recombinant proteins 4D5scFv-miniSOG and DARPin-miniSOG during their internalization in the complex with the HER2 receptor is their shielding and absorption of the fluorescence of miniSOG by the cells fluorophores.
About the authors
E. O. Kuzichkina
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry; Moscow Institute of Physics and Technology (State University)
Author for correspondence.
Email: kuzichkinazhenya@mail.ru
Russian Federation, Moscow, 117997; Dolgoprudnyi, Moscow oblast, 141700
O. N. Shilova
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: kuzichkinazhenya@mail.ru
Russian Federation, Moscow, 117997
S. M. Deyev
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: kuzichkinazhenya@mail.ru
Russian Federation, Moscow, 117997
R. V. Petrov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry
Email: kuzichkinazhenya@mail.ru
Russian Federation, Moscow, 117997
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