Email this article Development of an Immunosorbent for Solid-Phase NMR-Based Assay
- Authors: Khramtsov P.V.1,2, Kropaneva M.D.1, Bochkova M.S.1, Timganova V.P.1, Zamorina S.A.1,2, Rayev M.B.1,2
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Affiliations:
- Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences
- Perm State National Research University
- Issue: Vol 484, No 1 (2019)
- Pages: 69-72
- Section: Biochemistry, Biophysics, and Molecular Biology
- URL: https://journal-vniispk.ru/1607-6729/article/view/212791
- DOI: https://doi.org/10.1134/S1607672919010174
- ID: 212791
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Abstract
The conditions for constructing an immunosorbent reagent for solid-phase NMR analysis were optimized. For this purpose, we increased the area of the sensitized portion of the membrane to fit the relaxometer coil size and added the agent sorption buffer. This provided the penetration of the anti-ligand molecules into the membrane thickness and their uniform distribution.
About the authors
P. V. Khramtsov
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences; Perm State National Research University
Author for correspondence.
Email: khramtsovpavel@yandex.ru
Russian Federation, Perm, 614081; Perm,
614600
M. D. Kropaneva
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences
Email: khramtsovpavel@yandex.ru
Russian Federation, Perm, 614081
M. S. Bochkova
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences
Email: khramtsovpavel@yandex.ru
Russian Federation, Perm, 614081
V. P. Timganova
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences
Email: khramtsovpavel@yandex.ru
Russian Federation, Perm, 614081
S. A. Zamorina
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences; Perm State National Research University
Email: khramtsovpavel@yandex.ru
Russian Federation, Perm, 614081; Perm,
614600
M. B. Rayev
Institute of Ecology and Genetics of Microorganisms,Ural Branch, Russian Academy of Sciences; Perm State National Research University
Email: khramtsovpavel@yandex.ru
Russian Federation, Perm, 614081; Perm,
614600
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