Evaluation of the Effect of Surgical Access on the Innate Immune Response During Liver Resections of Various Volume, Experimental Study
- Authors: Panchenkov D.N.1,2,3, Alikhanov R.B.4, Ivanov Y.V.5, Akhmatova N.K.6, Kosyi V.V.7, Astakhov D.A.7, Leonov S.D.8
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Affiliations:
- Evdokimov Moscow State University of Medicine and Dentistry
- Skobelkin State Scientifi c Center of Laser Medicine the Federal Medical and Biological Agency of Russia
- Federal Research Clinical Center for Specialized Health Care and Medical Technologies of Federal Medical Biological Agency
- GBUZ "Moscow Clinical Scientific and Practical Center named after A.S. Loginov of the Moscow Department of Health"
- Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies
- Federal State Budgetary Scientific Institution "Research Institute of Vaccines and Serums named after I. I. Mechnikov"
- FSBEI HE "A.I. Evdokimov Moscow State Medical and Dental University" of the Ministry of Health of the Russian Federation
- Federal State Budgetary Institution "Scientific and Practical Center for Laser Medicine named after O.K. Skobelkin" of the Federal Medical and Biological Agency of Russia
- Issue: Vol 16, No 2 (2023)
- Pages: 150-155
- Section: Original articles
- URL: https://journal-vniispk.ru/2070-478X/article/view/233102
- DOI: https://doi.org/10.18499/2070-478X-2023-16-2-150-155
- ID: 233102
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Abstract
Introduction. The major components of the immune system are affected in almost all surgical operations, including those related to the tumor removal. The volume of the operation correlates with the level of the immune system suppression and frequency of complications. The issue of the effect of surgical access for liver resection with different volumes on surgical trauma and severity of the innate immune response are still challenging nowadays.
The aim of the study is to evaluate the effect of open and laparoscopic surgical approaches on the severity of the innate immune response in small and large liver resections in laboratory animals.
Methods. The study included 4 groups of rabbits, 10 animals each, formed depending on the surgical approach and the volume of liver resection: laparoscopic small and large liver resections, open small and large liver resections. The following parameters of the innate immune response were studied: phagocytic, cytotoxic and proliferative activity in dynamics. The experimental data were processed using the STATISTICA 6.0 software package (StatSoft, 2001). The results were considered statistically significant at p<0.05.
Results. Open extensive liver resections are characterized by a more significantly decreased phagocytic activity of neutrophils - from 95.7% to 32.05% - than similar interventions using laparoscopic access - from 96.4% to 52.4%, respectively. Cytotoxic activity in the group of animals exposed to surgery with laparotomy access decreased by 40% compared to 23% in animals exposed to laparoscopy surgery. There was registered a more than twice increased spontaneous and decreased induced proliferation in the postoperative period.
Conclusion. The study results allow concluding on a positive effect manifested as a reduced surgical injury for liver resections of various volumes due to laparoscopic access; this is evidenced by the parameters of immune reactivity.
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##article.viewOnOriginalSite##About the authors
Dmitry Nikolaevich Panchenkov
Evdokimov Moscow State University of Medicine and Dentistry; Skobelkin State Scientifi c Center of Laser Medicine the Federal Medical and Biological Agency of Russia; Federal Research Clinical Center for Specialized Health Care and Medical Technologies of Federal MedicalBiological Agency
Email: dnpanchenkov@mail.ru
ORCID iD: 0000-0001-8539-4392
SPIN-code: 4316-4651
M.D., Professor, Head of the Department of Surgery and Surgical Technologies, Laboratory of Minimally Invasive Surgery; Advisor to the Director of the
Skobelkin State Scientifi c Center of Laser Medicine; Surgeon Department of Surgery
Ruslan Bogdanovich Alikhanov
GBUZ "Moscow Clinical Scientific and Practical Center named after A.S. Loginov of the Moscow Department of Health"
Email: r.alikhanov@mknc.ru
ORCID iD: 0000-0002-8602-514X
Candidate of Medical Sciences, Head of the Department of Liver and Pancreatic Surgery, GBUZ "Moscow Clinical Scientific and Practical Center named after A.S. Loginov of the Moscow Department of Health"
Russian Federation, w. Enthusiasts, 86, Moscow, 111123Yury Viktorovich Ivanov
Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies
Email: ivanovkb83@yandex.ru
ORCID iD: 0000-0001-6209-4194
SPIN-code: 3240-4335
Doctor of Medical Sciences, Professor, Honored Doctor of the Russian Federation, Head of the Department of Surgery, Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies, FMBA of Russia; Chief Researcher, Laboratory of Minimally Invasive Surgery, Technobiomed Research Institute, Moscow State Medical University named after A.I. Evdokimova Ministry of Health of Russia
Russian Federation, 28 Orekhovy Boulevard, Moscow, 115682Nelly Kimovna Akhmatova
Federal State Budgetary Scientific Institution "Research Institute of Vaccines and Serums named after I. I. Mechnikov"
Email: anelly@mail.ru
ORCID iD: 0000-0002-4212-0276
SPIN-code: 5172-1223
MD, Head of the Laboratory of Mechanisms of Regulation of Immunity, FGBNU "Research Institute of Vaccines and Serums named after I.I. Mechnikov"
Russian Federation, Maly Kazenny per., 5A, Moscow, 105064Valentina Vasilyevna Kosyi
FSBEI HE "A.I. Evdokimov Moscow State Medical and Dental University" of the Ministry of Health of the Russian Federation
Email: kvv24@mail.ru
ORCID iD: 0000-0002-4628-2328
Applicant of the Department of Surgery and Surgical Technologies of the Federal State Budgetary Educational Institution of Higher Education "A.I. Evdokimov Moscow State Medical and Dental University" of the Ministry of Health of the Russian Federation; Junior Researcher, Federal State Budgetary Institution "Scientific and Practical Center for Laser Medicine named after O.K. Skobelkin" of the Federal Medical and Biological Agency of Russia
Russian Federation, Delegate str., 20/1, Moscow, 127473Dmitry Anatolyevich Astakhov
FSBEI HE "A.I. Evdokimov Moscow State Medical and Dental University" of the Ministry of Health of the Russian Federation
Email: astakhovd@mail.ru
ORCID iD: 0000-0002-8776-944X
SPIN-code: 6203-5870
Candidate of Medical Sciences, Associate Professor, Department of Surgery and Surgical Technologies, FSBEI HE "A.I. Evdokimov Moscow State Medical and Dental University" of the Ministry of Health of the Russian Federation; Senior Researcher, Federal State Budgetary Institution "Scientific and Practical Center for Laser Medicine named after O.K. Skobelkin" of the Federal Medical and Biological Agency of Russia
Russian Federation, Delegate str., 20/1, Moscow, 127473Sergey Dmitrievich Leonov
Federal State Budgetary Institution "Scientific and Practical Center for Laser Medicine named after O.K. Skobelkin" of the Federal Medical and Biological Agency of Russia
Author for correspondence.
Email: leonov-serg@yandex.ru
ORCID iD: 0000-0002-8883-9649
Senior Researcher, FSBI "Scientific and Practical Center for Laser Medicine named after O.K. Skobelkin" Federal Medical and Biological Agency of Russia
Russian Federation, Student str., 40, p. 1, Moscow, 121165References
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