Comparison of MALDI-ToF mass spectrometry identification accuracy of Mycobacterium abscessus complex strains, isolated on various nutrient media
- Authors: Alekseev D.V.1, Kargina E.A.1, Kokorev D.A.1, Kovalyov A.M.1, Borodulina E.A.1, Lyamin A.V.1, Ismatullin D.D.1
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Affiliations:
- Samara State Medical University
- Issue: Vol 14, No 6 (2024)
- Pages: 1227-1232
- Section: SHORT COMMUNICATIONS
- URL: https://journal-vniispk.ru/2220-7619/article/view/283041
- DOI: https://doi.org/10.15789/2220-7619-COM-17650
- ID: 283041
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Abstract
Background. Mycobacterium abscessus complex is one of the most abundant groups of rapidly growing non-tuberculous mycobacteria that has been increasingly more common causing infections of various localization, especially in cystic fibrosis (CF) patients. Microbiological diagnosis of such infections in case of using matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometry is often complicated due to mycobacterial cell features, which requires to perform a diagnostic optimization. The aim of the study was to evaluate the accuracy of Mycobacterium abscessus strains identification isolated on universal chromogenic medium and selective medium for Burkholderia cepacia complex (BCC) isolation. Materials and methods. Total number of 64 strains were selected for the study cultured in parallel on universal chromogenic medium and selective medium for BCC isolation. The identification of isolated microorganisms was carried out using the MALDI-ToF mass spectrometry on Microflex LT device. Statistical data processing was carried out using the StatTech program v.2.1.0. Results. The correlation analysis between identified data and used nutrient media was carried out showing that identification of mycobacteria isolated on chromogenic medium vs. medium for BCC isolation was more accurate. Conclusion. The study revealed that the composition of the nutrient medium affects the accuracy of MABSc member identification, which can be taken into account while developing protocols for optimizing and increasing the accuracy for this group of bacteria using MALDI-ToF mass spectrometry. Despite this, in the context of such a complex pathology with high comorbidity as CF, taking into account the universality of chromogenic medium we studied and often polymicrobial nature of infections in CF, it is rational to use selective media for primary inoculation of the studied material, including the medium for BCC isolation. However, after the initial inoculation, mycobacteria can be subcultured on chromogenic medium to assess cultural properties and improve the quality of species identification.
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##article.viewOnOriginalSite##About the authors
D. V. Alekseev
Samara State Medical University
Email: d.a.kokorev@samsmu.ru
Specialist of Laboratory of Cultural and Proteomic Research in Microbiology, Research and Educational Professional Center for Genetic and Laboratory Technologies
Russian Federation, SamaraE. A. Kargina
Samara State Medical University
Email: d.a.kokorev@samsmu.ru
6th year student of Institute of Pediatrics
Russian Federation, SamaraDaniil A. Kokorev
Samara State Medical University
Author for correspondence.
Email: d.a.kokorev@samsmu.ru
Specialist of Laboratory of Human Metagenomics of Professional Center for Education and Research in Genetic and Laboratory Technologies
Russian Federation, SamaraA. M. Kovalyov
Samara State Medical University
Email: d.a.kokorev@samsmu.ru
PhD (Biology), Head of Laboratory of Genetic Technologies in Microbiology of Professional Center for Education and Research in Genetic and Laboratory Technologies
Russian Federation, SamaraE. A. Borodulina
Samara State Medical University
Email: d.a.kokorev@samsmu.ru
DSc (Medicine), Head of Department of Phthisiology and Pulmonology
Russian Federation, SamaraA. V. Lyamin
Samara State Medical University
Email: d.a.kokorev@samsmu.ru
DSc (Medicine), Associate Professor, Director of Research and Educational Professional Center for Genetic and Laboratory Technologies
Russian Federation, SamaraD. D. Ismatullin
Samara State Medical University
Email: d.a.kokorev@samsmu.ru
PhD (Medicine), Head of Llaboratory of Research and Educational Professional Center for Genetic and Laboratory Technologies
Russian Federation, SamaraReferences
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