Polymorphism of microsatellite loci of cattle of the Limousine breed bred in the Republic of Bashkortostan
- Autores: Sedykh T.A.1,2, Gareeva D.I.1,2, Subkhankulov N.R.1, Kosilov V.I.3, Zorin D.N.4, Gladkikh M.Y.4, Selionova M.I.4
-
Afiliações:
- Ufa Branch of the Russian Academy of Sciences, Bashkir State Agrarian Institute
- Bashkir State Pedagogical Institute named after M. Akmulla
- Orenburg State Agrarian University
- Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazev
- Edição: Volume 17, Nº 4 (2025)
- Páginas: 223-249
- Seção: Biochemistry, Genetics and Molecular Biology
- ##submission.datePublished##: 31.10.2025
- URL: https://journal-vniispk.ru/2658-6649/article/view/351961
- DOI: https://doi.org/10.12731/2658-6649-2025-17-4-1207
- ID: 351961
Citar
Texto integral
Resumo
Background. Modern molecular genetic technologies make it possible to monitor genetic resources, both at individual and population levels, and reduce the time of genetic improvement of herds.
Purpose. The given research aims to study the polymorphism of microsatellite loci in Limousine beef cattle bred in the Republic of Bashkortostan. The objectives include analysing the polymorphism of microsatellite loci in Limousine cattle, produced by absorptive crossing of Limousine bulls with dual-purpose cows (Simmental and Bestuzhev) and investigating the genetic structure and indicators of genetic diversity of Limousine cattle subpopulations.
Materials and methods. The research objects are young Limousine cattle of absorptive crossing grown in the limited liability farm "Miasnoi soyuz bashkirskikh proizvoditelei (Meat Union of Bashkir Producers) and SEC Yaroslavsky. The research was conducted in the genetic laboratories of the Bashkir State Pedagogical University named after M. Akmulla, the Federal State Budgetary Educational Institution of the Higher Education the Russian State Agricultural University named after K.A. Timiryazev and Bashkir Agricultural Research Institute of the Ufa Federal Research Centre of the Russian Academy of Sciences.
Results. The analysis of 16 STR loci in Limousine cattle DNA revealed 116 alleles in group I, where the maternal foundation was of the Simmental breed, and 74 alleles in group II, where the maternal foundation was of the Bestuzhev breed. The average number of alleles per locus was 7.25 in group I, and 4.63 in group II. The mean number of effective alleles per locus in groups I and II was 4.14 and 3.27, respectively. Group I exhibited high Shannon index values in the loci TGLA227, BM2113, TGLA53, CSSM66, and INRA023. The observed and expected heterozygosity levels showed no significant differences. The spatial arrangement of genotypes in the principal coordinate system indicated that the studied subpopulations represent well-consolidated groups, with the preservation of individual genetic diversity in individuals, united by common ancestry and belonging to the same breed.
Conclusion. The obtained results can be used for the rational use of genetic resources of cattle of the Limousine breed and the development of genetically based breeding programs.
Palavras-chave
Sobre autores
Tatiana Sedykh
Ufa Branch of the Russian Academy of Sciences, Bashkir State Agrarian Institute; Bashkir State Pedagogical Institute named after M. Akmulla
Autor responsável pela correspondência
Email: nio_bsau@mail.ru
ORCID ID: 0000-0002-5401-3179
Rússia, 71, Oktyabrya Ave., Ufa, 450054, Russian Federation; 3a, Oktiabrskoi revolutsii Str., Ufa, 450008, Russian Federation
Diana Gareeva
Ufa Branch of the Russian Academy of Sciences, Bashkir State Agrarian Institute; Bashkir State Pedagogical Institute named after M. Akmulla
Email: diana.razgar@mail.ru
ORCID ID: 0009-0008-8279-6538
Rússia, 71, Oktyabrya Ave., Ufa, 450054, Russian Federation; 3a, Oktiabrskoi revolutsii Str., Ufa, 450008, Russian Federation
Niyaz Subkhankulov
Ufa Branch of the Russian Academy of Sciences, Bashkir State Agrarian Institute
Email: niyazsubhankulov@gmail.com
ORCID ID: 0000-0001-5038-2551
Rússia, 71, Oktyabrya Ave., Ufa, 450054, Russian Federation
Vladimir Kosilov
Orenburg State Agrarian University
Email: kosilov_vi@bk.ru
ORCID ID: 0000-0003-4754-1771
Rússia, 18, Cheliuskintsev Str., Orenburg, 460014, Russian Federation
Dmitry Zorin
Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazev
Email: ethodmitry@mail.ru
ORCID ID: 0000-0002-5189-1355
Rússia, 49, Timiryazevskaya Str., Moscow, 127550, Russian Federation
Marianna Gladkikh
Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazev
Email: marianna.gladkikh@rgau-msha.ru
ORCID ID: 0000-0002-2304-6058
Rússia, 49, Timiryazevskaya Str., Moscow, 127550, Russian Federation
Marina Selionova
Russian State Agrarian University – Moscow Agricultural Academy named after K.A. Timiryazev
Email: selionova@rgau-msha.ru
ORCID ID: 0000-0002-9501-8080
Rússia, 49, Timiryazevskaya Str., Moscow, 127550, Russian Federation
Bibliografia
- Dzhulamanov, K. M., Selionova, M. I., & Gerasimov, N. P. (2018). Genetic characteristics of the Hereford cattle population. Bulletin of Bashkir State Agrarian University, (4(48)), 59–64. https://doi.org/10.31563/1684-7628-2018-48-4-59-64. EDN: https://elibrary.ru/YVBCJN
- Dunin, I. M., Tyapugin, S. E., Meshcherov, R. K., Khodykov, V. P., Adzhibekov, V. K., Tyapugin, E. E., & Dyuldina, A. V. (2020). State of beef cattle breeding in the Russian Federation: realities and prospects. Milk and Meat Cattle Breeding, (2), 2–7. https://doi.org/10.33943/MMS.2020.40.30.001. EDN: https://elibrary.ru/TPIWMS
- Kalashnikova, L. A., Tyapugin, S. E., Novikov, A. A., & Grigoryan, L. N. (2022). State of the gene pool in pedigree livestock breeding in the Russian Federation. Zootechny, (12), 13–16. https://doi.org/10.25708/ZT.2022.12.85.004. EDN: https://elibrary.ru/IMAXVW
- Kayumov, F. G., & Tretyakova, R. F. (2023). Microsatellite DNA analysis of Kalmyk cattle raised in the Republic of Kalmykia. Proceedings of Orenburg State Agrarian University, (4(102)), 261–265. https://doi.org/10.37670/2073-0853-2023-102-4-261-265. EDN: https://elibrary.ru/YROYCV
- Kosilov, V. I., Tolochka, V. V., Kalyakina, R. G., Bykova, O. A., Gizatullin, R. S., & Ermolova, E. M. (2021). Effect of crossbreeding cattle of different productivity types on meat quality of crossbreds. Agrarian Bulletin of Primorye, (2(22)), 34–38. EDN: https://elibrary.ru/STQKNC
- Lubennikova, M. V., Afanasyev, V. A., & Afanasyev, K. A. (2024). Polymorphism of microsatellite markers in the Shebalino population of marals. Bulletin of Ulyanovsk State Agricultural Academy, (1(65)), 163–169. https://doi.org/10.18286/1816-4501-2 Newton-1-163-169. EDN: https://elibrary.ru/UPQOEE
- Nasambaev, E. G., Beishova, I. S., Ulyanova, T. V., & Chernyaeva, S. A. (2023). Study of the genetic structure of Hereford cattle using microsatellite markers. Science and Education, (2-1(71)), 74–82. https://doi.org/10.52578/2305-9397-2023-2-1-74-82. EDN: https://elibrary.ru/GGXULB
- Nikolaev, S. V., & Yaluga, V. L. (2024). Genetic characteristics of Holmogor bulls depending on their linear affiliation and level of Holsteinization. Agrarian Science, (6), 77–81. https://doi.org/10.32634/0869-8155-2024-383-6-77-81. EDN: https://elibrary.ru/LHZIEY
- Nikolaev, S. V., & Yaluga, V. L. (2023). Comparative genetic characteristics of microsatellite profile of Holsteinized and purebred Holmogor bulls. Agrarian Science, (7), 58–62. https://doi.org/10.32634/0869-8155-2023-372-7-58-62. EDN: https://elibrary.ru/MMSGBJ
- Samodelkin, A. G., Basonov, O. A., Asadchiy, A. A., & Kozakov, A. V. (2018). Productivity of crossbreds of various genotypes in absorption crossing of Black-and-White cows with Hereford bulls. Bulletin of Nizhny Novgorod State Agricultural Academy, (4(20)), 38–42. EDN: https://elibrary.ru/YXKHXN
- Sedykh, T. A., Gladyr, E. A., Dolmatova, I. Yu., Volkova, V. V., Gizatullin, R. S., & Zinovieva, N. A. (2014). Polymorphism of microsatellite loci in Hereford cattle of various ecological-genetic generations. Bulletin of AIC Stavropol, (3(15)), 121–128. EDN: https://elibrary.ru/SZBVNB
- Stolpovsky, Yu. A., Beketov, S. V., Solodneva, E. V., & Kuznetsov, S. B. (2024). Preservation of genetic resources of farm animals. Chief Zootechnician, (3(248)), 3–18. https://doi.org/10.33920/sel-03-2403-01. EDN: https://elibrary.ru/CWYNQN
- Tyapugin, S. E., Kalashnikova, L. A., Novikov, A. A., & Semak, M. S. (2022). Genetic examination of breeding products in livestock using blood groups and DNA markers. Zootechny, (5), 2–5. https://doi.org/10.25708/ZT.2022.20.69.001. EDN: https://elibrary.ru/MNGTYS
- Kharzinova, V. R., Kudryavtsev, A. V., Semerikova, M. N., & Zinovieva, N. A. (2023). Study of population structure and genetic diversity of Chukotka reindeer breed based on microsatellite analysis. Achievements of Science and Technology of AIC, (9(37)), 87–92. https://doi.org/10.53859/02352451_2023_37_9_87. EDN: https://elibrary.ru/VBESRE
- Chasovshchikova, M. A., & Sheveleva, O. M. (2018). Genetic characteristics of Hereford cattle in Tyumen Oblast using microsatellite DNA markers. Bulletin of IrGSXA, (88), 141–150. EDN: https://elibrary.ru/VKFEGI
- Shamina, O. V. (2023). The role of beef cattle breeding in forming Russia’s meat balance. Russian Journal of Management, 2(11), 184–190. https://doi.org/10.29039/2409-6024-2023-11-2-184-190. EDN: https://elibrary.ru/YGHQNW
- Shatalina, O. S., Tkachenko, I. V., & Yaryshkin, A. A. (2021). Genetic structure of the Holsteinized Black-and-White cattle population based on microsatellite loci. Genetics, 57(2), 205–213. https://doi.org/10.31857/S0016675821020090. EDN: https://elibrary.ru/HUEAQD
- Bora, Sh., Tessema, T., & Girmay, G. (2023). Genetic diversity and population structure of selected Ethiopian indigenous cattle breeds using microsatellite markers. Genetics Research, 2023, 1106755. https://doi.org/10.1155/2023/1106755. EDN: https://elibrary.ru/WRLLJG
- Petrov, A., & Kamaldinov, E. (2024). Genetic structure of cattle of the Siberian branch by microsatellite loci. Bulletin of NSAU (Novosibirsk State Agrarian University), 72(3), 230–239. https://doi.org/10.31677/2072-6724-2024-72-3-230-239. EDN: https://elibrary.ru/OUEOOC
- Stevanovic, J., Stanimirovic, Z., Dimitrijevic, V., & Maletic, M. (2010). Evaluation of 11 microsatellite loci for their use in paternity testing in Yugoslav Pied cattle (YU Simmental cattle). Czech Journal of Animal Science, 55, 221–226. https://doi.org/10.17221/183/2009-CJAS
- Ubushieva, V., Gorlov, I., Chimidova, N., & Ubushieva, A. (2024). Microsatellite analysis of Kalmyk cattle. RUDN Journal of Agronomy and Animal Industries, 19, 12–18. https://doi.org/10.22363/2312-797X-2024-19-1-12-18. EDN: https://elibrary.ru/BIJLFM
Arquivos suplementares


