The role of nitric oxide in the mechanisms of stress-protective action of low-intensity extremely high-frequency electromagnetic radiation in acute and chronic stress
- Authors: Ravaeva M.Y.1, Cheretaev I.V.1, Chuyan E.N.1, Galenko-Yaroshevsky P.A.2, Zelenskaya A.V.2
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Affiliations:
- V.I. Vernadsky Crimean Federal University
- Kuban State Medical University
- Issue: Vol 22, No 4 (2024)
- Pages: 377-387
- Section: Original study articles
- URL: https://journal-vniispk.ru/RCF/article/view/283521
- DOI: https://doi.org/10.17816/RCF635404
- ID: 283521
Cite item
Abstract
BACKGROUND: The study of stress mechanisms is an area of current interest in physiology and medicine. Understanding the role of nitric oxide (NO) in the mechanism of stress-protective action of low-intensity extremely high-frequency electromagnetic radiation (EHF EMR) requires investigating the main components of the stress response.
AIM: To determine the role of NO in the development of the anti-stress effect of low-intensity EHF EMR.
MATERIALS AND METHODS: 140 male Wistar rats weighing 200–220 g were divided into the following seven groups of 20 animals each: intact animals; AS, animals after a single exposure to acute stress; EHF-AS, animals irradiated with EHF EMR for 10 days and exposed to acute stress on day 10; L-NAME-EHF-AS, animals who were administered the NOS inhibitor L-NAME (10 mg/kg, intraperitoneally) for 10 days and irradiated with EHF EMR after 1 hour; HK, animals exposed to chronic hypokinetic stress for 10 days; EHF-HK, animals exposed to EHF EMR and hypokinetic stress for 10 days; L-NAME-EHF-HK, animals who were administered the NOS inhibitor L-NAME (10 mg/kg, intraperitoneally) for 10 days, irradiated with EHF EMR after 1 hour, and exposed to hypokinetic stress after another 1 hour. The levels of middle-weight molecules (MWM), circulating immune complexes (CIC), and malondialdehyde (MDA) were determined in the blood serum.
RESULTS: Acute stress increased the level of malondialdehyde, activating lipid peroxidation processes; in the EHF-AS group, this effect disappeared. NO blockade in the L-NAME-EHF-AS group activated endotoxication processes, the stress cascade, and physiologically active regulatory peptides (increase in the MWM level at 280 nm), oxidative metabolism of proteins and lipids (increase in the CIC and MDA levels), and inhibited immunogenesis. Hypokinetic stress suppressed the stress inhibiting system of the body and physiologically active regulatory peptides (decrease in the MWM level at 280 nm), suppressed immunogenesis, and increased oxidative metabolism of proteins and lipids (increase in the CIC and MDA levels). The stress-protective effect of EHF EMR was observed in the EHF-HK group. NO blockade in the L-NAME-EHF-HK group activated endotoxication processes, stress cascade and stress inhibiting systems of the body, physiologically active regulatory peptides (increase in the MWM level at 254 and 280 nm), oxidative metabolism of proteins and lipids (increase in the CIC and MDA levels), and inhibited immunogenesis.
CONCLUSIONS: NO blockade with L-NAME amplifies the effects of acute and hypokinetic stress under the exposure to low-intensity EHF EMR. Normal NO levels are necessary for the stress-protective effect of EHF EMR to develop during acute and hypokinetic stress.
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##article.viewOnOriginalSite##About the authors
Marina Yu. Ravaeva
V.I. Vernadsky Crimean Federal University
Author for correspondence.
Email: ravaevam@yandex.ru
ORCID iD: 0000-0002-6081-1628
SPIN-code: 2398-3901
Cand. Sci. (Biology)
Russian Federation, 4 Akademika Vernadckogo av., Simferopol, 295007Igor V. Cheretaev
V.I. Vernadsky Crimean Federal University
Email: cheretaev86@yandex.ru
ORCID iD: 0000-0003-1852-4323
SPIN-code: 4587-6492
Cand. Sci. (Biology)
Russian Federation, 4 Akademika Vernadckogo av., Simferopol, 295007Elena N. Chuyan
V.I. Vernadsky Crimean Federal University
Email: elena-chuyan@rambler.ru
ORCID iD: 0000-0001-6240-2732
SPIN-code: 8373-3871
Dr. Sci. (Biology)
Russian Federation, 4 Akademika Vernadckogo av., Simferopol, 295007Pavel A. Galenko-Yaroshevsky
Kuban State Medical University
Email: galenko.yarochevsky@gmail.com
ORCID iD: 0000-0003-0873-284X
SPIN-code: 1575-6129
Dr. Sci. (Medicine), Corresponding Member of the Russian Academy of Sciences
Russian Federation, KrasnodarAnait V. Zelenskaya
Kuban State Medical University
Email: anait_06@mail.ru
ORCID iD: 0000-0001-9512-2526
SPIN-code: 7646-3620
MD, Cand. Sci. (Medicine)
Russian Federation, KrasnodarReferences
- Chuyan EN, Ravaeva MYu, Mironyuk IS et al. Tissue microhemodynamics: mechanisms of influence of low-intensity electromagnetic radiation of the millimeter range. Technologies of Living Systems. 2024;21(1):29–45. EDN: GIHJUF doi: 10.18127/j20700997-202401-03
- Chuyan EN, Tribrat NS, Ravaeva MYu, Ananchenko MN. Tissue microhemodynamics: the influence of low-intensity electromagnetic radiation of millimeter range. Simferopol: ARIAL; 2017. 422 p. (In Russ.) EDN: YSFPUY
- Vladyka AS, Levickii ER, Poddubnaya LP, Gabriehlyan NI, et al. Middle molecules and the problem of endogenous intoxication in critical states of various etiologies. Anesthesiology and Reanimatology. 1987;(1–3):37–42. (In Russ.)
- Gabrielyan NI, Levitsky EN, Dmitriev AA, et al. Screening method for determination of middle molecules in biological fluids. Moscow: Medicine, 1985. 18 p. (In Russ.)
- Lewis SM, Bain BG, Bates I. Dacie and Lewis practical haemotology. Philadelphia: Elsevier; 2006. 722 p.
- Grinevich YA, Alferov AN. Determination of immune complexes in the blood of oncological patients. Laboratornoe delo. 1981;(8): 493–496. (In Russ.)
- Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978;86(1):271–278. doi: 10.1016/0003-2697(78)90342-1
- Vladimirov YuA, Archakov AI. Lipid peroxidation in biological membranes. Moscow: Nauka; 1972. 252 p. EDN: PJBHRZ
- Drannik GN. Clinical immunology and allergology. Odessa: AstroPrint; 1999. 603 p.
- Pyataev NA, Kotlov IS, Boyarinov GA, Kuzin VV. Diagnostic and prognostic value of various markers of endogenous intoxication in peritonitis. Efferent Therapy. 2002;8(2):49–52. (In Russ.)
- Krotenko NM, Alifirova VM, Krotenko NV, et al. Middle-mass molecules and antibodies to double- and single-stranded DNA in patients in different clinical forms of multiple sclerosis. Fundamental Research. 2012;(5):305–310. EDN: PBACUB
- Chalenko VV. Possible reasons for the increase in the concentration of middle mass molecules in pathology. Pathologic Physiologic Experiment. 1991;(4):13–14. (In Russ.)
- Aksenova VM, Kuznetsov VF, Maslov YuN, et al. Biochemical methods of diagnostics of endogenous intoxication. Perm: PGMA; 2005. 48 p. (In Russ.) EDN: RPHKMD
- Yudakova OV, Grigoriev EV. The intensity of lipid peroxidation and of antioxidant activity and the level of average-weight molecules as indicators of endogenous intoxication in generalized peritonitis. Clinical Laboratory Diagnostics. 2004;(10):20. (In Russ.) EDN: MXXIJM
- Gromashevskaya LL. «Middle molecules» as one of the indicators of ‘metabolic intoxication’ in the body. Laboratory diagnostics. 1997;(1):11–16. (In Russ.)
- Nikolskaya VA, Danilchenko YV, Memetova ZN. Biochemical aspect of consideration of middle mass molecules in the body. Scientific Notes of the V.I. Vernadsky Taurida National University. Biology. Chemistry. 2013;26(1):139–145. (In Russ.) EDN: VEBBNJ
- Dorokhin KM, Spas VV. Pathophysiologic aspects of endogenous intoxication syndrome. Anesthesiology and Reanimatology. 1994;(1):56–60. (In Russ.) EDN: ELSLUU
- Ilyinsky IA, Lukinskaya TV. Circulating immune complexes in salmonellosis. Immunology. 1994;(4):105–108. (In Russ.)
- Safronov ID, Knyazeva VO, Kuleshov VM, Trunov AN. Effect of electromagnetic radiation of extremely high frequencies on the state of pro-/antioxidant balance in women with late termination of pregnancy. In: Proceedings of the international symposium “Heliogeophysical factors and human health”, Novosibirsk, November 15–16, 2005. Novosibirsk: Editorial and Publishing Center; 2005. P. 134. (In Russ.) EDN: YGNKBF
- Bichekueva FH, Hutueva SH. Clinical and immunological assessment of the effectiveness of EHF therapy in complex treatment of bronchial asthma in mountainous climate. Cytokines and inflammation. 2008;7(3):37–38. (In Russ.)
- Trunov AN, Kuleshov VM, Safronov ID, Knyazeva VO. The effect of electromagnetic radiation of extremely high frequencies on the state of humoral immunity in women with late termination of pregnancy. In: Proceedings of the international symposium “Heliogeophysical factors and human health”, 2005 November 15–16, Novosibirsk, Russia; Novosibirsk: Editorial and Publishing Center; 2005. P. 133–134. (In Russ).
- Nielsen F, Mikkelsen BB, Nielsen JB, et al. Plasma malondialdehyde as biomarker for oxidative stress: reference interval and effects of life-style factors. Clin Chem. 1997;43(7):1209–1214.
- Klychnikova EV, Tazina EV, Rey SI, et al. Evaluation of prognostic significance for biochemical markers of oxidative stress, endogenous intoxication and vascular regulation in the development of unfavorable outcomes in patients with sepsis. Russian Sklifosovsky Journal of Emergency Medical Care. 2016;(2):25–30. EDN: WELJDP
