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Post a Comment Morphofunctional features of platelets in premature newborns with very low and extremely low body weight
- Authors: Budalova A.V.1, Kharlamova N.V.1, Kuzmenko G.N.1
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Affiliations:
- Federal State Budget Institute Ivanovo scientific-research institute named after V.N. Gorodkov
- Issue: Vol 12, No 2 (2021)
- Pages: 35-41
- Section: Original studies
- URL: https://journal-vniispk.ru/pediatr/article/view/76600
- DOI: https://doi.org/10.17816/PED12235-41
- ID: 76600
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Abstract
Background. Currently, the development of medicine in the field of perinatology is aimed at improving the quality of medical care for newborns, especially those born prematurely. Premature newborns are most likely to develop hemorrhagic disorders, which often aggravates their condition and determines high morbidity and mortality. On modern hematological analyzers, it has become possible to evaluate a larger number of blood parameters, including platelet parameters, however, there are a small number of studies devoted to the study of platelet parameters in premature newborns.
The aim was to study the morphofunctional features of platelets in premature newborns with very low and extremely low body weight.
Materials and methods. The study included 78 newborns born at 25–34 weeks of gestation, with a body weight of less than 1500 grams. On the 3rd–5th day of life, a clinical blood test was performed on the Advia 2120i hematological analyzer (Siemens), with the determination of platelet parameters: PLT, ×103 cells/µl, PCT, %, PDW, %, Large Plt, ×103 cells/µl, MPC, g/dl, MRM, pg.
Results. In newborns with ELBW, a decrease in the number and granularity of platelets in the blood, an increase in the average dry mass of platelets was found. Newborns with intrauterine growth retardation have a reduced platelet count and reduced thrombocrit. In premature newborns with a gestational age of 25–27 weeks, blood circulation of platelets with a reduced volume was established, and in newborns who were born at a gestational age of 32–34 weeks with increased granularity. In the absence of antenatal prevention of respiratory distress syndrome in newborns, there is a decrease in thrombocrit, the number of platelets, including large forms in the blood. In premature newborns born in a state of severe asphyxia, a decrease in platelet granularity was noted. The use of high concentrations of O2 in the oxygen-air mixture during respiratory therapy leads to a decrease in the number of platelets in the blood.
Conclusions. Factors determining the morphofunctional state of platelets in premature newborns were established: the presence of a full course of antenatal prevention of respiratory distress syndrome of newborns, gestational age, the severity of asphyxia at birth, as well as the concentration of O2 in the oxygen-air mixture used in respiratory therapy. Newborns with ELBW have a reduced platelet count, low-granulated platelets, and an increased average dry platelet mass. Newborns with intrauterine growth retardation have a reduced number of platelets and thrombocrit in the blood. The revealed morphofunctional features of platelets allow us to clarify the nature of changes in the platelet link of hemostasis in premature newborns for the timely prevention of complications during the underlying disease.
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##article.viewOnOriginalSite##About the authors
Anastasia V. Budalova
Federal State Budget Institute Ivanovo scientific-research institute named after V.N. Gorodkov
Author for correspondence.
Email: nastena110492@yandex.ru
Postgraduate Student, Department of Obstetrics and Gynecology, Neonatology, Anesthesiology and Resuscitation
Russian Federation, IvanovoNatalia V. Kharlamova
Federal State Budget Institute Ivanovo scientific-research institute named after V.N. Gorodkov
Email: nataliakhar13@yandex.ru
MD, PhD, Dr. Sci. (Med.), Leading Scientist, Department of Neonatology and Clinical Neurology
Russian Federation, IvanovoGalina N. Kuzmenko
Federal State Budget Institute Ivanovo scientific-research institute named after V.N. Gorodkov
Email: kuzmenko_gnk@mail.ru
MD, PhD, Dr. Sci. (Med.), Leading Scientist, Laboratory of Clinical Biochemistry and Genetics
Russian Federation, IvanovoReferences
- Будалова А.В., Харламова Н.В., Кузьменко Г.Н., и др. Морфометрические особенности тромбоцитов у недоношенных новорожденных // Материалы XIII Всероссийского образовательного конгресса «Анестезия и реанимация в акушерстве и неонатологии». – М., 2020. – С. 58–59. [Budalova AV, Kharlamova NV, Kuz’menko GN, et al. Morfometricheskie osobennosti trombotsitov u nedonoshennykh novorozhdennykh. Proceedings of the Russian science conference “Anesteziya i reanimatsiya v akusherstve i neonatologiI”; Moscow, 2020. P. 58-59. (In Russ.)]
- Кузьменко Г.Н., Назаров С.Б., Попова И.Г., и др. Инновационная технология оценки гемостатического потенциала крови недоношенных новорожденных // Российский педиатрический журнал. – 2015. – Т. 18. – № 2. – С. 4–10. [Kuzmenko GN, Nazarov SB, Popova IG, et al. Innovative technology in the evaluation of the haemostatic potential of blood in preterm neonates. The Russian Pediatric Journal. 2015;18(2): 4-10. (In Russ.)]
- Леонтьев М.А., Родзаевская Е.Б., Масляков В.В. Морфология тромбоцитов новорожденных (обзор литературы) // Вестник медицинского института «РЕАВИЗ»: реабилитация, врач и здоровье. – 2017. – № 6. – С. 75–82. [Leontyev MA, Rodzayevskaya EB, Maslyakov VV. Platelet morphology in neonates (a literature review). Bulletin of the Medical Institute “REAVIZ” (Rehabilitation, doctor and health). 2017;(6):75-82. (In Russ.)]
- Мининкова А.И. Аналитические возможности гематологических анализаторов в оценке тобоцитов (обзор литературы) // Клиническая лабораторная диагностика. – 2012. – № 3. – С. 27–34. [Mininkova A.I. The analytical possibilities of hematologic analyzers in evaluation of thrombocytes: a literature review. Russian Clinical Laboratory Diagnostics. 2012;(3):27-34. (In Russ.)]
- Счастливцев И.В., Лобастов К.В., Цаплин С.Н., Мкртычев Д.С. Современный взгляд на систему гемостаза: клеточная теория // Медицинский совет. – 2019. – № 16. – С. 72–77. [Schastlivtsev IV, Lobastov KV, Tsaplin SN, Mkrtychev DS. Modern view on hemostasis system: cell theory. Medical Council. 2019;(16):72-77. (In Russ.)]
- Харламова Н.В., Чаша Т.В., Малышкина А.И., и др. Медицинская помощь детям, родившимся на сроке гестации 27 недель и менее // Неонатология: новости, мнения, обучение. – 2015. – № 4. – С. 31–32. [Kharlamova NV, Chasha TV, Malyshkina AI, et al. Meditsinskaya pomoshch’ detyam, rodivshimsya na sroke gestatsii 27 nedel’ i menee Neonatology: News, Opinions, Training. 2015;(4):31-32. (In Russ.)]
- Харламова Н.В., Матвеева Е.А., Шилова Н.А., и др. Состояние здоровья новорожденных детей с очень низкой и экстремально низкой массой тела // Материалы межрегиональной научно-образовательной конференции, посвященной 45-летию организации детской специализированной службы Ивановской области. – Иваново, 2017. – C. 107–108. [Kharlamova NV, Matveeva EA, Shilova NA, et al. Sostoyanie zdorov’ya novorozhdennykh detei s ochen’ nizkoi i ehkstremal’no nizkoi massoi tela. Materials of the interregional scientific and educational conference, posvyashchennoi 45-letiyu organizatsii detskoi spetsializirovannoi sluzhby Ivanovskoi oblasti. Ivanovo, 2017. P. 107-108. (In Russ.)]
- Giacomini A, Legovini P, Gessoni G, et al. Platelet count and parameters determined by the Bayer ADVIATM 120 in reference subjects and patients. Clin Lab Haematol. 2001;23(3):181-186. https://doi.org/10.1046/j.1365-2257.2001.00391.x
- Giacomini A, Legovini P, Antico F, et al. Assessment of in vitro platelet activation by Advia 120 platelet parameters. Lab Hematol. 2003;9(3):132-137. https://doi.org/10.1046/j.1365-2257.2001.00391.x
- Szpecht D, Szymankiewicz M, Nowak I, Gadzinowski J. Intraventricular hemorrhage in neonates born before 32 weeks of gestation-retrospective analysis of risk factors. Childs Nerv Syst. 2016;32:1399-1404. https://doi.org/10.1007/s00381-016-3127-x.
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