Email this article Proteolytic Activity of Chymotrypsin Immobilized on Selenium Nanoparticles
- Authors: Plyushchenko A.V.1,2, Borovikova L.N.1, Pisarev O.A.1,2
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Affiliations:
- Institute of Macromolecular Compounds
- Peter the Great St. Petersburg Polytechnic University
- Issue: Vol 54, No 4 (2018)
- Pages: 375-378
- Section: Article
- URL: https://journal-vniispk.ru/0003-6838/article/view/152527
- DOI: https://doi.org/10.1134/S0003683818040117
- ID: 152527
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Abstract
Chymotrypsin was immobilized as a part of hybrid organo-inorganic nanocomplexes on selenium nanoparticles in the reduction reaction of selenious acid with ascorbic acid. Chymotrypsin stabilized the selenium nanoparticles in the solution. The spectral characteristics of synthesized nanocomplexes and the proteolytic activity of immobilized chymotrypsin were studied. It was shown that the immobilized enzyme had increased proteolytic activity in an alkaline medium.
About the authors
A. V. Plyushchenko
Institute of Macromolecular Compounds; Peter the Great St. Petersburg Polytechnic University
Email: chrom79@yandex.ru
Russian Federation, St. Petersburg, 199004; St. Petersburg, 195251
L. N. Borovikova
Institute of Macromolecular Compounds
Email: chrom79@yandex.ru
Russian Federation, St. Petersburg, 199004
O. A. Pisarev
Institute of Macromolecular Compounds; Peter the Great St. Petersburg Polytechnic University
Author for correspondence.
Email: chrom79@yandex.ru
Russian Federation, St. Petersburg, 199004; St. Petersburg, 195251
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