Email this article Whole-cell bacterial biosensors for the detection of aromatic hydrocarbons and their chlorinated derivatives (Review)
- Authors: Plotnikova E.G.1,2, Shumkova E.S.1,3, Shumkov M.S.3
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Affiliations:
- Perm State National Research University
- Institute of Ecology and Genetics of Microorganisms
- Bach Institute of Biochemistry, Fundamental Principles of Biotechnology, Federal Research Center
- Issue: Vol 52, No 4 (2016)
- Pages: 347-357
- Section: Article
- URL: https://journal-vniispk.ru/0003-6838/article/view/151945
- DOI: https://doi.org/10.1134/S0003683816040128
- ID: 151945
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Abstract
The review summarizes the data on new directions in biosensor technologies based on whole bacterial cells. Biosensors for the monitoring of mono(poly)aromatic hydrocarbons and their chlorinated derivatives, which are constructed with genetically modified bacterial cells bearing a reporter gene fusion, are considered. The operating principle of these biosensors is based on the expression of reporter genes (luc, lux, gfp, rfp) under the control of a promoter and a regulator that specifically respond to a detected compound.
About the authors
E. G. Plotnikova
Perm State National Research University; Institute of Ecology and Genetics of Microorganisms
Author for correspondence.
Email: peg_el@mail.ru
Russian Federation, Perm, 614990; Perm, 614081
E. S. Shumkova
Perm State National Research University; Bach Institute of Biochemistry, Fundamental Principles of Biotechnology, Federal Research Center
Email: peg_el@mail.ru
Russian Federation, Perm, 614990; Moscow, 119071
M. S. Shumkov
Bach Institute of Biochemistry, Fundamental Principles of Biotechnology, Federal Research Center
Email: peg_el@mail.ru
Russian Federation, Moscow, 119071
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