


Vol 42, No 3-4 (2016)
- Year: 2016
- Articles: 22
- URL: https://journal-vniispk.ru/1068-3674/issue/view/14201
Plant Growing
Diploid-tetraploid crosses as the instrument for obtaining apomictic maize plants
Abstract
In this paper, the opportunity to reveal pseudogamous apomixis in maize inbred lines when tetraploids are used as pollinators is demonstrated for the first time. Kernels with hybrid endosperm, from which plants of maternal type developed, were obtained in five of ten studied lines. Hybrid nature of these kernels was proven using SDS electrophoresis of endosperm proteins. In addition to apomictic kernels, the normally developed kernels with di-, three- and tetraploid hybrid embryos were obtained.



Seed germination energy of various rice varieties and its connection with shoot generation
Abstract
The seed germination energy of different rice varieties at low temperature and its relation to the seed germination rate and morphological traits of seedlings have been studied. According to the obtained results, rice varieties significantly differ in the seed germination energy at 14°C; under these conditions, this parameter is closely connected with the shoot density and weight of seedlings.



Polymorphism of microsatellite loci in sugar beet’s selection lines
Abstract
The genetic polymorphism of 12 microsatellite loci was revealed and unique DNA-profiles were obtained for initial lines of sugar beet. Two loci were monomorphic, other loci exhibited polymorphism. The genetic distances between the breeding materials were determined; the dendrogram of cluster analysis reflecting the probable divergence of samples was constructed. The data about the distance of the selection material will be used in the selection of pairs in heterotic breeding.



Use of electrophoretic spectra of reserve seed proteins to improve typicalness of sunflower parental lines
Abstract
This paper shows the possibility of the efficient individual plant selection from sunflower parentallines by allelic variants of components of electrophoretic spectra of reserve proteins. The plants have been evaluated and selected by electrophoretic spectra of reserve proteins and, at the same time, evaluated by morphological features, which has ensured increased genetic homogeneity of parentalline ZL95A. Moreover, the genetic purity of sterile line alternative Ast, determined by ground control, is 98.4% and the genetic purity of line sterility fixer Af is 98.5%. The typicalness of the sterile alternative and the sterility fixer defined by allelic variants of components of electrophoretic spectra of reserve proteins is 100%.



Genetic shifts in selection of hybrid offspring soybeans to economic-valuable signs
Abstract
The article presents the importance of plant genetic hybrid shift in the elements of productivity, allowing the prediction of the conduct in the selection of promising lines obtained by interspecific hybridization spontaneous soybeans for further study in the selection process. The maximum shift of genetic selection on the basis of elements of efficiency in terms of the number of marked beans in combinations Volzhan × KZ-671, KZ-Rosinka × 1236, and Luch nadezhdy × KZ-6332 in terms of the mass of 1000 seeds only in the combination Luch nadezhdy × KZ-6332.



Organic and inorganic fertilization influenced on yield and quality of sugar beet genotypes
Abstract
The objective of this study was to evaluate the effects of organic and inorganic fertilizers on yield and quality of sugar beet genotypes (Beta vulgaris L.). Therefore a field trial was carried out at New Developmental Farm of The University Agriculture, Peshawar, Pakistan during winter 2012–13. The field experiment was layout in randomized complete block design with split plot arrangement having three replications. Fertilizers treatments (control, higo organic plus (composted manure, it contains N 2%, P 3%, K 3%, organic matter 40%, organic carbon 11%, Zn 145 mg kg–1, Cu 56 mg kg–1, Fe 380 mg kg–1 and Mg 228 mg kg–1), maxicrop sea gold (extract of sea weeds i.e., Sargasssum, Laminara Polysaccharide and Ascophyllum Nodosum), farm yard manure, NP (90: 60 kg ha–1), NP (120: 90 kg ha–1) and NP (150: 120 kg ha–1) were allotted to main plots while genotypes (Sandrina, Serenada and Kawe Terma) to the sub plots. Farm yard manure (10 t ha–1) and higo organic Plus (5 t ha–1) was incorporated in the soil before seed bed preparation. Maxicrop sea gold (5 lit ha–1) was sprayed after the emergence of the crop. All phosphorus was applied @ of 60, 90 and 120 kg ha–1 at the time of sowing while nitrogen @ of 90, 120 and 150 kg ha–1 in two splits/3 of the dose was applied at the time of sowing while the remaing 1/3 of the dose was applied before earthen up. Plots treated with application of NP ratio 120: 90 kg ha–1 produced maximum beet yield (76.4 t ha–1), sugar yield (11.1 t ha–1), Pol (polarizable sugar) percentage (14.67%) and more economic return (Rs.234 Thousand ha–1) as compared to control plots. Sugar beet genotype Serenada had significantly produced maximum beet yield (55.5 t ha–1), sugar yield (7.9 t ha–1), pol (polarizable sugar) percentage (14.60%), brix percentage (14.60%) and more economic return (158) as compared to other genotypes. It was concluded from the above results that sugar beet genotype Serenada treated with NP ratio 120: 90 kg ha–1 for improved sugar beet productivity and quality therefore it is recommended for general practice in agro-climatic conditions of Peshawar valley.



Growth and fatty acid composition of sesame (Sesamum indicum L.) genotypes as influence by planting dates and nitrogen fertilization in semiarid region of Northwest, Pakistan
Abstract
A field experiment was conducted on silty clay loam soil in the years 2011–2012. Two sesame (Sesamum indicum L.) cultivars (Local Black and Local White) were evaluated using various 3 different sowing dates (20th June, 10th and 30th July) and four agrotechnical level (0, 40, 80 and 120 kg N ha–1) at New Developmental Farm The University of Agriculture, Peshawar, Pakistan. The objective of this study was to evaluate the effects of sowing dates on growth, yield and oil fatty acid composition of two sesame cultivars grown under different nitrogen fertilization. Results showed that cv. Local Black was characterized by significantly higher content of oil (47%), seed yield (696 kg ha–1) and oil yield (335 kg ha–1) while cv. Local White had higher palmitic acid (8%) and linoleic acid (38.7%). Yield and its main components were positively affected by the earlier sowing date. With regard to fatty acid composition, a decrease in oleic and stearic acid and an increase in linoleic and palmitic acid were observed. At early sowing, oleic and palmitic acid decreased whereas linoleic and stearic acid increased. The decrease in the oleic/linoleic acid ratio observed at early sowing, suggests a possible role of temperature on the activity of oleate desaturase in the developing seeds. Intensive technology of cultivation (120 kg N ha–1), compared to the economical technology (40 kg N ha–1), significantly increased the seed yield of both sesame cultivars. This was due to higher number of branches, Capsules m–2, capsules plant–1, seeds capsule–1 and 1000 seed weight (g). The intensive technology of cultivation had a beneficial effect on the content of palmitic acid, linolenic acid and oleic acid in sesame seed.



Characteristics of interspecific hybrids between Pleurotus ostreatus and P. eryngii
Abstract
In this study, three species of oyster mushroom (Pleurotus osturatus, P. eryngii and P. cornucopia) were crossed together in order to aggregate benefit special attributes to the genotype (s). The monokaryon of each of these species was prepared. Then, the monokaryons of two species were placed at 5 mm distance from each other to produce dikaryon. The results showed that, from among 700 crosses, only the crosses of eryngii and osturatus monokaryons were grown toward each other and produced clamps (dikaryons). Four produced hybrids were noted H1, H32, H11 and H40. The spans of produced hybrids were prepared; then, they were grown into sterile media and attributions of hybrids were studied. The results indicated that H11 hybrid was an appropriate hybrid in terms of the number of days until growing, number of days until the observation of the first pin and the days from planting to harvest. However, H40 was the best hybrid based on cap diameter, dry and fresh weight of fruit body, yield and biomass. It is expected that these interspecific hybrids employ for future oyster mushroom breeding programs.



Plant Protection
Evaluation of effect of insecticides on beneficial arthropods
Abstract
This paper presents the results of evaluating the effect of such insecticides as imidacloprid, thiacloprid, chlorantraniliprol, and thiamethoxam on representatives of beneficial arthropods, including Miridae, Anthocoridae, Cecidomyiidae. The insect mortality was determined upon treatment with insecticides in recommended application rates. The results of laboratory and field studies of the effect of insecticides on beneficial arthropods are compared.



Efficiency assessment of herbicides aimed at protecting spring rape against gramineous weeds in the Kaluga oblast
Abstract
This paper provides the efficiency assessment of herbicides based on hizalophone-P-ethyl against gramineous weeds Miura KE and Galera 334 intermixed with spring rape plantings in the Kaluga oblast. However, there was no phytotoxic effect of these weeds on cultivated plants discovered during vegetation. It is shown that the herbicides applied in amounts exceeding 0.8 L/ha produce the most efficient exterminating effect on one-year and perennial gramineous weeds.



Bioenergetics
Halophytes are promising sources for producing biofuel
Abstract
The that the arid regions of Russia and Central Asiatic countries have an inexhaustible potential for the development of the raw materials base of biofuel power engineering is shown: vast territories of yet uncultivated lands, surplus of solar energy, large reserves of saline water, and significant genetic resources of halophytes that are plants with maximum resistance to salt stress. According to the data of experiments, in case of irrigation with saline water, halophytes form 15–18 and more t/ha of dry biomass in the arid regions of Russia, Uzbekistan, and Kazakhstan, which is comparable with lucerne irrigated with fresh water. The task of expanding and deepening the research into the use of halophyte resources for obtaining biofuel in the arid regions of Russia is stated to be urgent.



Fodder Production
Reaction of cultivars and populations of alfalfa on heat and drought in Volga Region
Abstract
The harvest of seed and green mass of alfalfa cultivars and population went down meaningfully during the last 2 years (2014, 2015). This period was characterized by higher values of average air temperature (by 1.0°С) and decreased relative air humidity (by 6.0%) and rainfall during the vegetation of alfalfa (by 107.0 mm). New varieties Sirena and Natali, as well as populations 2/08 and 6/04, were characterized by the highest yield of green mass and seeds in competitive variety trial.



Agrochemistry. Soil Science
Variation in main morphometric parameters of chernozems under anthropogenic impact
Abstract
Data characterizing the distribution pattern of the main morphometric parameters of chernozems in the Kamennaya Steppe are given. Variable thickness of humus horizon within a flat test plot typical for the central part of the chernozemic zone of Russia is shown. It has been revealed that annually exerted strong anthropogenic impact causes a pronounced drop in the thickness of humus horizon. The investigated objects on the test plot—fallow land, plowed filed, and forest shelter belt—were situated side by side. The statistical parameters of the thickness of humus horizon in different subtypes of chernozems have been calculated. They mainly differ by the depth of continuous impregnation with calcium carbonate (effervescence depth varies from 1 to 95 cm). The deepest effervescence is seen in the soils of the fallow and under the forest belt (68 and 63 cm, respectively).



Effect of long-term fertilization on the nitrogen status of leached chernozem and the nitrogen balance in a grain–beet crop rotation
Abstract
It has been revealed that the 70-year-long application of fertilizers to a sugar beet crop rotation in the forest-steppe of the Central Chernozemic Zone has significantly affected the nitrogen status of leached chernozem and increased the content of mineral nitrogen and the mobility of nitrogen compounds. High application rates of mineral fertilizers create a positive nitrogen balance in the crop rotation and could minimize the losses of total nitrogen in the future.



Evaluation of the fertilizer values of stored and fresh human urine in experimental mixed culture of fish and prawn
Abstract
With a view to closing the nutrient loop between sanitation and fish culture, advanced fry of different species of fish (rohu, bata, mrigal, common carp, tilapia, punti) and larvae of freshwater prawn were reared in twelve experimental tanks in a mixed culture system for 120 days using three treatments (fresh urine, stored urine and mix of fresh and stored human urine) and control in triplicate. The total weight for all the species of fish and prawn in the stored urine (420.0 g) was 18 and 27% higher compared to fresh (356.0 g) and mixed urine (332.0 g) treatments, respectively. Likewise, primary productivity of phytoplankton and heterotrophic bacterial load related to phosphate level of water were also highest and lowest in the stored urine (GPP—508 ± 39.87 mg C m–2 h–1; heterotrophic bacteria—38.38 cfu × 103 mL–1) and control system (GPP—214 ± 38.09 mg C m–2 h–1; heterotrophic bacteria—13.53 cfu × 103 mL–1), respectively. The mean count of E. coli, on the other hand, did not differ between urine fed treatment and control. As a possible mechanism, it is suggested that stored urine upon transfer to aquaculture pond underwent degradation and mineralization that induced the autotrophic and heterotrophic food webs conducive to fish growth.



Animal Husbandry
Hematological indices of Aginskaya semicourse wool sheep
Abstract
Research findings in the morphological and biochemical indices of blood of semicourse wool sheep of the Aginskaya breed, according to different sex and age groups, have been reported. It is ascertained that ewes surpass the young ewes and young rams in the blood erythrocytes concentrations, the hemoglobin levels, and hematocrit levels by 77.8 and 39.1%, 44.3 and 40.5%, and 63.8 and 48.5%, respectively. Some excess of the physiological norm limits within the biochemical indices of blood serum was indicated.



Meat quality of broiler chickens affected by cadmium toxicity
Abstract
A 114 g decrease in the live weight gain and a 160 g increase in the mixed feed intake may become evident at the fifth maximum permitted level (MPL) for cadmium added to a broiler-chicken diet. The concentration of the pollutant increased two and a half as high in meat. A high concentration of the pollutant in the mixed fodder could cause decrease in the contents of muscle tissue fat, lysine and leucine, and vitamins B2, B6, and B12 by 6.4, 2.6–4.0, and 1.0–8.0%, respectively. In addition, the level of water reliably became approximately one and a half higher in tissues; the contents of threonine and vitamin B5 increased by 20.5 and 0.92%, respectively; the concentrations of chlorine and potassium decreased by 2.5 and 4.2%, respectively; the contents of iron and manganese increased by 1.7 and 1.8%, respectively.



Veterinary
Cytokine profile of albino rats’ changes under the impact of Salmonella against the background of subacute T-2 toxicosis
Abstract
The results of the study demonstrated the immunosuppression in albino rats under a complex impact of T-2 toxin and Salmonella, manifested by the significant increase of anti-inflammatory cytokine IL-1β content in the blood and a slight increase of IL-4, stimulating the humoral immunity, the decrease of concentration of other anti-inflammatory mediators (TNF-α, IL-8), immunoregulatory interleukins IL-2, interferon-γ and anti-inflammatory cytokine IL-10, and interferon–γ/IL-4 ratio, indicating an evident T-deficit and a more significant suppression of Th-1 lymphocyte function.



Studying the antagonistic activity of Lactobakterin against Rhodococcus equi in vivo
Abstract
The high antagonistic activity of the probiotic Lactobakterin against Rhodococcus equi in in vitro experiments was the base for studying this probiotic in vivo. In the course of the researches, the intravenous injection of Lactobakterin into white mice was approved and the advisability of this method in the conditions of the experiment was shown. The antagonistic activity of the probiotic Lactobakterin was studied on the mouse model against the virulent strain of Rhodococcus equi. It was stated that the antagonistic activity of the probiotic is 1.25-fold higher under the three-fold intravenous injection into the mice infected intraperitoneally with the strain of Rhodococcus equi than under the 2-fold injection. The intravenous injection of the probiotics is possible upon the condition of their short-term application during the critical periods for the animal.



Determination of EC50 of water liozol of turpentine oil for acid eryhtrogram
Abstract
The cytotoxic in vitro effect of water turpentine oil liozols, intended as adjuvants for a number of vaccines, is investigated by the acid erythrogram method. It is found out that the acid resistance of erythrocytes does not change at a turpentine oil concentration below 0.0013 g/L in liozol and increases at a concentration of 0.0013–0.0125 g/L. If the concentration continues to increase, however, the acid resistance of erythrocytes decreases to reach the value of EC50 at a concentration of 0.1–0.125 g/L; then follows the zone of direct hemolytic action of liozol.



Modeling
Artificial neural network for crop classification using C-band RISAT-1 satellite datasets
Abstract
The artificial neural network algorithm has been used for the classification of rice, corn, pigeon pea, green gram, other crop and non-crop classes in Varanasi District, Uttar Pradesh, India. The C-band, dual polarimetric, temporal satellite datasets of RISAT-1 have been carried out in the present study. The separability analysis using transformed divergence and Jefferies Matusita distance methods were compared. The transformed divergence method has shown better separation between the classes in comparison to Jefferies Matusita distance method. The classification results were ground validated. The overall achieved accuracies were 74.21 and 77.36% for satellite data acquired on 9 August 2013 and 28 September 2013 respectively. Results have shown the better classification accuracy using 28th September 2013 data because of almost crops were in the reproductive stage and high reflectivity of the crops at this stage.



Melioration
Effect of changes in the Caspian Sea level on ecological-ameliorative status of lands of the Mugan–Salyan massif
Abstract
The effect of rising Caspian Sea level on the environment has been studied. The changes in the work of the collecting-drainage network and in the ecological-ameliorative status of lands in the affected zone have been analyzed. The sea transgression has resulted in the groundwater rise at a distance of 20–30 km from the sea, which caused the disturbance of the work of the collecting-drainage system in the Mugan–Salyan massif located in the Caspian Sea zone. The modified groundwater regime initiated quick soil salinization.


