Volume 25, Nº 2 (2025)
Radiophysics, Electronics, Acoustics
Ensembles of four discrete phase oscillators
Resumo
134-146
Recovery of compartment model parameters of dynamical systems for the epidemiological SIR model
Resumo
147-156
Optics and Spectroscopy. Laser Physics
Application of Au10 gold nanoclusters to block the formation of DNA from non-canonical forms
Resumo
157-166
Physics of Condensed Matter
Quantum shims in a strong magnetic field – carriers of electronic states with controllable quantum numbers
Resumo
167-177
Simulation the response of graphene to far-infrared pulses
Resumo
178-188
Biophysics and Medical Physics
Sensing using SERS-substrate and machine learning approaches
Resumo
189-200
Differences in the kinetics of optical clearing of healthy and diabetic head tissues
Resumo
201-210
Solid-State Electronics, Micro- and Nanoelectronics
Developed ternary processor units based on analog models of ternary logic elements
Resumo
211-221
Nanotechnologies, Nanomaterials and Metamaterials
Characterization of imprinted albumin by molecular modelling and spectroscopy
Resumo
Background and Objectives: Imprinted proteins are promising alternatives to natural recognition systems, such as biological receptors or antibodies. However, the knowledge available on the theoretical study of imprinted proteins as recognition systems is limited. In this study, bovine serum albumin (BSA) is imprinted in the presence of 4-hydroxycoumarin. Change in protein structure is studied by molecular modelling and spectroscopy. Materials and Methods: To evaluate the effect of pH on the structural properties of BSA during imprinting, fluorescence 2D and 3D spectroscopy and dynamic light scattering (DLS) combined with molecular dynamics and metadynamics simulations were carried out to monitor the conformational change of the protein matrix. Results: Analysis of molecular dynamics simulation has shown that the mechanism of BSA conformational state changes is associated with displacements of molecular domains relative to each other. Based on molecular dynamics data, the values of collective variables have been selected for mapping the free energy of the system. The distance and angle between the centers of mass of domains D1 and D3 have been specified as collective variables. Simulations using themetadynamics method have been performed for 100 ns. As a result, slices of the potential energy surface have been obtained. Analysis of the free energy surface shows that 3.05 nm and 1.53 radian correspond to the minimum energy (ΔG = –6.14 kJ·mol–1). BSA have been studied by fluorescence and DLS. DLS analysis has shown that BSA exists predominantly in monomeric form in solution. In acidic media (pH 3.0) the significant changes of fluorescence properties of BSA have been observed. The results of molecular modelling are consistent with the experimental results. Conclusion: An important practical result of this study is that the theoretical study of such molecular systems combined with fluorescence characterization during synthesis can be applied to control imprinting process and to create new imprinted proteins with a wide range of applications.
222-229
The effectiveness of the effect of microwave radiation and convection heating on the relaxation of internal stresses in cured polymer composite materials
Resumo
230-241
Methodological Part
About the envelope of the wave packet (pulse)
Resumo
242-254
From the History of Physics
To the History of the Physical and Mathematical Research Institute of Saratov State University
Resumo
255-261

