Vol 108, No 1 (2025)
Physiology of animals
Peculiarities of ruminal digestion in situ when ultrafine particles of silicon dioxide and sulfur-containing compounds of organic and inorganic nature are introduced into the diet of the Kazakh white-headed bulls
Abstract
In the last few years, there has been a growing interest in the use of natural feed additives in animal feed. In particular, the prospects of using ultrafine particles as a substitute for antibiotics to change fermentation in the rumen and increase the efficiency of feeding ruminants are shown. The purpose of our study was to evaluate a sulfur-containing organomineral complex based on amino acids and Na2SO4 for the effectiveness of rumen digestion by the in situ method in its pure form and in combination with UFP SiO2. The study was conducted on Kazakh white-headed bull calves with chronic fistula. 3 groups were formed for in situ testing: I – control; II – amino acid complex (tryptophan, lysine, methionine) + Na2SO4; III – amino acid complex (tryptophan, lysine, methionine) + Na2SO4 + UFP SiO2. The study showed an increase in the coefficient of digestibility in groups II and III by 3.7 and 2.6% compared with the control. The analysis of volatile fatty acids showed an increase in the concentrations of acetic, propionic, and butyric acids by 112, 77.7, and 76.8% in group I and by 44.3; 4.52% and 32.6% were in II, respectively. Changes in protein metabolism were associated with an increase in protein and non-protein nitrogen in group I compared with the control by 85.6% and 52.3%. The study demonstrated the beneficial effect of combined use of the studied substances on the processes of rumen digestion. The combined use of an ultrafine form of silicon dioxide, an amino acid complex, and sodium sulfate proved to be effective in stimulating rumen digestion.



Dynamics of clinical blood parameters in donor heifers of the Kholmogor breed during consecutive sessions of transvaginal aspiration of follicles
Abstract
Monitoring of clinical blood parameters is of interest to identify possible early disorders of health status and optimize the use of donors for oocytes retrieval by transvaginal ultrasound-guided follicle puncture (OPU). The aim of the research was to study the effect of consecutive OPU sessions on the variability of clinical blood parameters in donor heifers of the Kholmogor breed. The research was carried out at the L.K. Ernst Federal Research Center for Animal Husbandry on heifers of the Kholmogor breed (n=7) at the age of 1.5 – 2 years. The experimental studies included three periods. In the first period, blood samples were taken on the 4th, 7th, 11th, 14th, 18th and 21st days of hormonally synchronized estrus cycle. In the second period, after repeated synchronization of the estrus cycle, the 1st series of nine consecutive OPU sessions was performed twice a week with alternating intervals of 3 and 4 days on the 4th, 7th, 11th, 14th, 18th, 21st, 25th, 28th and 32nd days the estrus cycle. In the third period, after a 1-month rest period, the 2nd series of nine consecutive OPU sessions was performed in a similar timing regimen. Blood samples were collected on the days of the OPU sessions. The values of clinical parameters were determined: the number of erythrocytes, the average volume of erythrocytes, hemoglobin content, hematocrit, the number of leukocytes and their distribution into lymphocytes, medium cells, including monocytes, basophils, eosinophils and their precursors, and granulocytes. Comparison of the values of clinical blood parameters on the days of OPU sessions with similar days of the estrus cycle, in which follicle aspiration was not performed, in most cases, did not reveal significant differences. Considering that the values of the studied clinical blood parameters were within the values of the indicators observed by a number of authors, the data obtained can be considered as an indication of the absence of a visible effect of intensive OPU sessions on the clinical status of donors. In this regard, a timing regime assuming two series of nine consecutive OPU sessions twice a week with a rest period of 1 month between series can be recommended for retrieval of oocytes from donor heifers of the Kholmogor breed.



Factors and modulators of the avian immune system (review)
Abstract
The modern development of poultry breeding is marked by genetic and breeding achievements aimed at increasing productivity and reducing rearing time. Prospects of modern research are directed to the search for new technologies that contribute to the increase of organism resistance and reveal genetic potential. The presented review systematizes data on structural and functional features of the immune system of poultry, considers etiological factors of immunosuppression and analyzes determinants affecting the formation of nonspecific resistance of the organism. The data on the key role of essential trace elements, including zinc, selenium, iron, iodine, manganese and copper, in the regulation of immune status are presented.



Breeding, selection, genetics
Reproduction traits of Simmental cows obtained from sires of local and imported lines
Abstract
The modern population of the Simmental breed in Russia is represented by different genotypes, and further breeding work is focused on obtaining animals mainly of dairy and meat direction of productivity using both domestic and imported sires of German and Austrian breeding. The combination of high milk productivity and good reproductive function of cows is an important criterion in breeding work with dairy cattle in general and in assessing their breeding value. Daughters born from Simmental bulls of local origin reach the age of fruitful insemination later (the age of the 1st calving is 32.5 months) and have a significantly shorter service period and intercalving period - 134.1 and 413 days, respectively, on average for three lactations, relative to the daughters from sires of Holstein lines and German-Austrian lines of imported origin. The value of the service period decreases with age regardless of the type of breeding orientation of the line: for the 1st lactation - 139.6...161.1 days, for the 2nd lactation – 131.3...148.7, for the 3rd lactation – 122.4...146.9 days. The study of indicators characterizing the reproductive function of Simmental cows obtained from local and imported breeding sires is an important aspect for evaluating and optimizing breeding work and allows us to determine the influence of the genotype of fathers on the reproductive potential of daughters.



Testing BLUP methodology for large white pigs selection
Abstract
The article examines the convergence of BLUP-based (Best Linear Unbiased Prediction) estimates of economically important traits in Large White pigs using different biometric models. The results indicate that significant differences in model quality, reflected particularly in coefficients of determination, lead to considerable discrepancies in evaluation outcomes. For instance, the highest convergence was observed for the FCR (feed conversion ratio) trait: at the population level, the repeatability coefficient was 0.981, with average rank differences for the 20 most reliably evaluated individuals amounting to ∆ = -19.05. Meanwhile, the difference in the coefficients of determination of the models used was 6.2%. The lowest convergence was observed for the BWG (body weight gain) trait, where the repeatability coefficient was 0.720, and the rank difference reached ∆ = 119.55. The coefficients of determination for the models used to assess this trait differed by 27%, or 1.81 times. Such discrepancies under simulated conditions of limited availability of primary zootechnical accounting data underscore both the necessity of considering the full range of available variables affecting the variability of the evaluated trait and the importance of using the same set of variables to ensure comparability of results.



Production technology, quality and economy in animal husbandry
Аge and hereditary factors affecting the hematological parameters of the Hereford cattle
Abstract
Blood testing of beef cattle is relevant due to the peculiarities of keeping animals in conditions requiring a high level of adaptation. Hereford cattle tolerate extremely low and high temperatures well; however, for breeding cattle it is important to evaluate animals not only by productivity, but also by interior features, including hematological parameters. The aim of the study was a comparative assessment of the blood parameters of breeding cattle of the Hereford breed in the parental and daughter generations. The study was conducted in a breeding farm located in the foothill zone of the Maslyaninsky District of the Novosibirsk Region. The object of the study was the hematological parameters of peripheral blood of Hereford cows and heifers (mother-daughter pairs) kept outdoors all year round in the foothill pastures of the south of Western Siberia. The age of the cows was 3-5 calving, the age of the heifers was 17-18 months. Blood counts in the maternal and daughter generations were analyzed, and the total number of parent-descendant pairs was 66 pairs. It has been established that the hematological parameters of cows and heifers in the conditions of foothill pastures in the south of Western Siberia are within the reference values with a sufficiently high variability of all signs. Significant differences between the indicators of mothers and daughters under the condition of the same type of maintenance, feeding and the period of blood sampling indicate the presence of the influence of the age of animals rather than environmental conditions. At the same time, a fairly high level of heritability of hematological parameters was found, primarily related to the blood's ability to transport oxygen, which can be used as a criterion for the animals' adaptability to extreme conditions.



Efficiency of using different schemes for synchronizing the sexual heat of heifers in beef cattle breeding
Abstract
In beef cattle breeding, the transfer of mature herd to a seasonal reproduction system takes time and may be accompanied by a decrease in the yield of young animals. To avoid this, various schemes for synchronizing sexual heat have been developed. Of these, the most effective are double administration of prostaglandins and a combination of prostaglandins with releasing hormone. But even so, the fertilisation rate from frontal insemination is on average 40 %. A reserve for increasing efficiency is the combined use of hormonal drugs and adaptogens in synchronization schemes. Four groups of the Kazakh White-Headed heifers of 40 heads each were formed for comparative tests. Sexual heat was synchronized in all groups according to the following protocols: in I group - a double injection of estrophane, in II group - a double injection of estrophane and two simultaneous injections of cresacin (adaptogen) solution, in III group - the Ovsynch scheme, in IV group - the Ovsynch scheme and two simultaneous injections of cresacin solution. Frontal insemination was performed in each group, fertilization was taken into account, and the economic effect of synchronization was calculated. The results of fertilization were 42.5% in I group, 47.5% in II group, 40.0% in III group, and 50.0% in IV group. The reduced fertility rate in III group is due to a stronger stress effect on animals when performing the Ovsynch scheme in beef cattle breeding. The maximum result was obtained in II and III groups, i.e. with the combined use of hormonal drugs and cresacin. When calculating economic indicators, similar results were obtained: the cost of maintaining infertile heifers decreased in I group by 76296.68 rubles, in II group by 83330.36 rubles, in III group by 72880.64 rubles, in IV group by 91642.4 rubles, Therefore, the inclusion of cresacin in the synchronization scheme of sexual heat of heifers is an economically beneficial measure.



Consequences of the heat stress in forage production and animal husbandry and ways to solve them (review)
Abstract
In the agro-industrial complex, in the conditions of an increasing number of extremely high summer droughts, there is an objective need for structurally significant changes in the feed production feedstock conveyor by introducing non-traditional drought-resistant forage crops - Sudan grass, sorghum and sorghum-Sudan hybrids, as well as forage crops with a short growing season for an uninterrupted supply of livestock with feed. Under heat stress, it is important to use properly selected high-quality feed and special feed additives that reduce the production of metabolic heat in the body, as well as to adjust feeding regimes, monitor the level of the temperature-humidity index, the indicators of which will allow a timely assessment of the need for cooling of animals. The article presents overview information on the state of forage production and livestock farming in conditions of extremely high temperatures. The papers of Russian and foreign authors on various practical strategies for mitigating the effects of heat stress in the maintenance and feeding of cows, which may be useful to specialists in the field of dairy farming, were reviewed.



Theory and practice of feeding
Modification of metabolism and productivity of dairy cattle using the enzyme-probiotic feed additive Profort
Abstract
Modern methods of feeding dairy cattle, including year-round uniform feeding, are not always optimal for ruminants, which can lead to a decrease in the efficiency of nutrient absorption, especially with excessive intake of concentrated feeds, in which a large amount of lactic acid is formed in the rumen microbiome as a result of carbohydrate fermentation, which has a negative effect on the metabolism, structure and function of internal organs. One of the possible solutions to this problem in feeding highly productive cows is a targeted effect on the symbiotic microflora of the forestomachs by feeding probiotic preparations. In this regard, the aim of our research was to study the effect of the enzyme-probiotic feed additive Profort on the metabolic status, milk productivity and milk quality of lactating cows under conditions of year-round uniform feeding. Analysis of the milk productivity of cows after the first feeding (within 15 days) of Profort showed that the average daily milk yield in animals increased by 6.1%, compared with animals in the control group. Milk productivity of cows after repeated feeding of the preparation remained higher by 4.6 % than in the control animals. After the end of the experiment and the next feeding of the preparation, the difference in average daily milk yield was 1.9 kg (4.9%) in favor of the cows of the experimental group. Gross milk yield during the experiment in the experimental group was higher by 5.94%. The use of the enzyme-pobiotic feed additive Profort improves liver function in lactating cows. Total protein indicators increased by 1.46%, albumins - by 16.5% (P≤0.05), AST activity decreased by 15% (P≤0.05) and ALT - by 17.5%; the level of urea, triglycerides and cholesterol decreased by 11, 20 and 30.5%, respectively, which indicates an improvement in the metabolic state.



The yeast Kluyveromyces and its biological potential (review)
Abstract
Today, it is relevant to use feed additives of microbial origin based on the simplest fungi in the nutrition of farm animals and poultry. The exclusion of feed antibiotics has provoked interest in feed yeast and the study of the effect of yeast cultures on the gastrointestinal tract, microflora and rumen function.
Kluyveromyces is a new non-traditional food and feed yeast that is isolated from various habitats, such as kefir grains, fermented dairy products, waste water from the sugar industry, and plants. A unique set of useful properties, namely rapid growth, thermotolerance and a wide range of substrates makes these yeasts especially attractive for use in various sectors of the food, pharmaceutical and biotechnology industries. Dairy yeasts are promising probiotic microorganisms that can break down lactose. In this regard, it is relevant to study the dairy yeast Kluyveromyces as a reliable source of yeast cells for the production of various enzymes, bioethanol, cellular proteins, probiotics, fructose and fructooligosaccharides, as well as vaccines with natural properties.



The effect of a feed additive combining anticoccidium and prebiotic preparations on the safety and growth of broiler chickens
Abstract
Complete balanced feeding of animals is one of the fundamental moments in increasing the production of livestock products and their quality indicators. In poultry farming, along with increasing productivity, an important task is the prevention of various diseases, in particular coccidiosis. The article presents the results of studies on the combined use of the prebiotic additive "LactuSuper" and the new anticoccidial drug Eimicide in feeding broiler chickens and their impact on growth rates, intestinal microbiome, development of the digestive organs and slaughter characteristics. As a result of the research, it was found that the combined use of anticoccidial and prebiotic preparations in broiler diets is more effective than separately, since the experimental animals of this particular group were distinguished by greater survival, average daily live weight gain, total microbial count in the blind processes of broiler chickens with a predominance of beneficial microflora, and all corrective changes in the mass of organs were aimed at strengthening the microbiocenosis of intestinal microflora, which contributed to obtaining a greater yield of carcasses, offal and edible parts of the carcass.



The effect of biologically active feed additives in the diet on the hematological parameters of fish
Abstract
Hematological analysis is one of the main tools for assessing the effect of various feed additives on the animal body. The article presents the results of the effect of biologically active feed additives on the growth and morpho-biochemical parameters of the blood of carp (Cyprinus carpio). For 42 days, in addition to the basic diet, vanillin (I experimental), probiotic (II experimental), vanillin + probiotic complex (III experimental), vanillin + ultrafine particles of silicon dioxide + probiotic (IV experimental) or vanillin + ultrafine particles of silicon dioxide + Zn + I + Cr + Co complex (V experimental), were included in the yearlings' diet. It was found that feed additives had a different effect on the body of fish. If in the I, II and V experimental groups an increase in live weight was recorded to 10.2% (P≤0.01), then in the III and IV experimental groups the growth of fish decreased relative to the control to 19.6% (P≤0.001). Morphological blood analysis showed that biologically active feed additives contributed to an increase (P≤0.05) in hemoglobin in all experimental groups, with the exception of groups III and V, where a decrease (P≤0.05) was recorded. In the study of biochemical parameters, a general trend of changes in a number of indicators was recorded in groups whose diet included probiotics. There were significant differences in the concentration of ALT, total protein, triglycerides and cholesterol. Thus, the effect of biologically active feed additives contributed to obtaining distinctive results. Positive results on hematological parameters were established in the I and V experimental groups, while ambiguous results were found in the II, III and IV groups.



Assessment of the degree of digestibility of feed when keeping dogs of service breeds on a ready-made diet
Abstract
The article analyzes data on the assessment of the digestibility of the ready-made Grand Prix diet based on turkey and beef in service dogs. The study found that the nutritional value, taking into account the digestibility of the ready-made diet, was not satisfied in all dogs of the analyzed sample: the protein digestibility coefficient averaged 39.04% in service dogs of the German Shepherd breed and 48.44% in dogs of the Belgian Shepherd breed (Malinois). The amount of fat in the ready-made diet was on average 2.6 times higher than the daily requirement of animals with a digestibility of 89.00%. The total digestibility coefficient for the main components of the feed was 63.54 % for the German Shepherd and 68.41% for the Belgian Shepherd (Malinois), while the calculated indicator according to the manufacturer was 88.09 %. Problematic issues of the practice of choosing food for service dogs according to the degree of digestibility are formulated.



Geoponics and crop production
The influence of weather conditions and cellulolytic activity of soil on the yield of durum wheat in the steppe zone of the Southern Urals
Abstract
The work is devoted to the problems of assessing the dependence of durum wheat productivity on a number of factors in unstable climatic conditions. In the presented work, the influence of air temperature, precipitation, dry wind days, cellulolytic activity and nitrate nitrogen content in the soil on the productivity of durum wheat in the system of six-field, two-field crop rotations and monoculture in the arid conditions of the Southern Urals was studied for the first time. Field experiments were carried out on a long-term experimental plot for crop rotations and monocultures. Durum wheat crops were placed in crop rotations and monoculture near the village of Nezhinka, Orenburg region (coordinates: 51.775125o N and 55.306547o E). Research (2002-2022) established average values of air temperature - 5.7-22.7 °C, precipitation - 22-364 mm, number of dry wind days - 68, cellulolytic activity of the soil - 0.69-0.76%, nitrate nitrogen content - 0.32-0.75 mg/100 g of soil, crop yield - 0.48-0.97 t/ha. A correlation was found between grain yield and air temperature in May, June (r = 0.49-0.65) and during the growing season (r = 0.40-0.47), with precipitation in May, June (r = 0.33-0.67) and during May-August (r = 0.44-0.60), with the number of dry wind days (r = 0.30-0.41), with the hydrothermal coefficient in May, June (r = 0.41-0.76) and during the growing season (r = 0.50-0.66). The lowest relationship between weather factors and yield was noted in July and August. A weak relationship was found between cellulolytic activity of the soil and yield (r = 0.17-0.31). A weak dependence of grain yield on the nitrate nitrogen content in the soil after sowing the crop (r = 0.13-0.34) and the difference (N-NO3) during the growing season (r = 0.01-0.17) was noted. In the arid conditions of the Southern Urals, a slowdown in the vital activity of microflora is noted due to a lack of soil moisture, which contributes to a decrease in the relationship between cellulolytic activity and the yield of spring durum wheat. Thus, weather conditions are fundamental in the formation of the yield of spring durum wheat.


