卷 20, 编号 2 (2017)
- 年: 2017
- 文章: 93
- URL: https://journal-vniispk.ru/1028-7221/issue/view/6239
Articles
FEATURES OF THE FUNCTIONAL STATE OF THE IMMUNE SYSTEM IN PERSONS SUBJECTED TO CHRONIC RADIATION EXPOSURE IN UTERO IN THE REMOTE PERIOD
摘要
The embryonic and fetal tissues are more radiosensitive than the tissues of an adult organism. Therefore, intrauterine irradiation is a greater danger compared to irradiation in the postnatal period. A complex assessment of the functional state of the immune system was carried out in the period of the long-term effects of chronic radiation exposure in persons irradiated in utero and in early childhood. Various violations of the immune system are shown in persons irradiated in the antenatal period of development, compared with people irradiated in the postnatal period.
Russian Journal of Immunology. 2017;20(2):93-96
93-96
THE ROLE OF ADENOSINTRIPOSPHATE IN THE REGULATION OF HUMAN PERIPHERAL BLOOD NEUTROPHILES AND MONOCYTES DEGRANULATION IN VITRO
摘要
At present, the metabolism of nucleotides is of great interest in the context of the regulation of functions of immunocompetent cells in inflammation. It is adenosine triphosphate that is attributed to many effects that cause chemotaxis, activation, degranulation of various subpopulations of leukocytes. In this study, the effect of ATP on the degranulation of neutrophils and various subpopulations of human peripheral blood monocytes in vitro was studied using flow cytometry. We have shown that the addition of ATP at concentrations of 1 mM and 0.1 mM caused a significant increase in the expression level of the СD66b degranulation marker on neutrophils. An increase in the expression level of the СD63 degranulation marker on a subpopulation of "classical" monocytes expressing СD14 is shown only at the time of 15 min of incubation, while subpopulations of "nonclassical" monocytes expressing СD16 did not respond to the application of this stimulant.
Russian Journal of Immunology. 2017;20(2):96-99
96-99
REACTIONS HAVE DIFFERENCES OF PHAGOCYTES OF PERIPHERAL BLOOD OF PATIENTS WITH DIFFERENT VOLUMES AFFECTED LUNG INFILTRATIVE TUBERCULOSIS
摘要
The purpose of research is the study of differences in the reactions of phagocytes of peripheral blood of patients with different lesion volume infiltrative pulmonary tuberculosis. The work was based on a study of the clinical and laboratory data of 44 patients with infiltrative pulmonary tuberculosis (29 patients were affected lung segment 1-3, 15 patients had tuberculosis 4 and lighter segments). The control group consisted of 25 healthy people. Used method - flow cytometry, Coulter*Epics*XL instrument (Beckman Coulter, USA). Evaluated absorbency peripheral blood phagocytes (monocytes and neutrophils), ability to oxidative exposure, the antigen-presenting and chemotactic functions. Statistical data analysis was performed using the «Microsoft Office Excel 2007" program and «Statistica For Windows v.6.1». It was found that the infiltrative tuberculosis 1-3 segments of the lung accompanied by an increase in the populations of monocytes and granulocytes which express the molecule CD11b adhesion. TB 4 or more segments of the lung is associated with an increase in the number of monocytes and granulocytes expressing CD11c, decrease monocyte production of superoxide anion. Immunological evaluation of lung volume criterion is the percentage of monocytes, producing superoxide anion.
Russian Journal of Immunology. 2017;20(2):100-103
100-103
DIFFERENTIATED APPROACH IN EVALUATING THE RESPONSE OF IMMUNE SYSTEM TO THE PRESENCE OF M.TUBERCULOSIS WITH DIFFERENT DRUG SUSCEPTIBILITY
摘要
The purpose of the study has a comparative study of quantitative and functional state of the immune system cells in patients with infiltrative pulmonary tuberculosis, which is caused by drug-sensitive and drug-resistant disease. The study included 29 patients, of which: from 12 people the disease was caused by drug-sensitive M.tuberculosis, 17 - the causative agent had multidrug resistance and 25 healthy people. We estimated the number of T- (CD45+CD3+) and B-lymphocytes (CD45+CD19+), NK-cells (CD45+CD3CD16+CD56+), T-helper cells (CD3+CD4+), cytotoxic T cells (CD3+CD8+), γδ-T-lymphocytes (CD3brightCD4-), NKT-cells (CD3+CD16+CD56+), activated T-lymphocytes and by the expression of T-helper cells of CD25 and HLA-DR, the probability of apoptosis of CD95 expression, the number of T-reg-cells (CD3+CD4+CD127CD25+) by flow cytometry on Coulter®Epics®XL instrument (Beckman Coulter, USA). Statistical data analysis was performed using Microsoft software, USA (Office Excel 2007) and StatSoft, USA (Statistica For Windows v.6.1.). Established that infiltrative tuberculosis caused by drug-sensitive M.tuberculosis, accompanied by an increase in HLA-DR expression and decrease in CD95 on T-lymphocytes, reducing the amount of B-cells, increased number of NK-cells. Infiltrative tuberculosis caused by M.tuberculosis multidrug resistance, is characterized by the absence of reducing CD95 on T-lymphocytes, a decrease in the population of NKT cells. Immunological criterion for evaluating drug sensitivity M.tuberculosis is the absolute quantity of CD3+HLA-DR+-cells.
Russian Journal of Immunology. 2017;20(2):103-106
103-106
ANALYSIS OF THE APOPTOTIC DEATH INDICATORS OF LYMPHOCYTES AMONG THE PERSONS EXPOSED TO CHRONIC IRRADIATION
摘要
The evaluation of the apoptotic death of lymphocytes among the inhabitants of the coastal villages of the Techa River exposed to long-term low-level irradiation was carried out. We analysed the number of lymphocytes in the stage of DNA fragmentation by the TUNEL method, the number of CD95+ lymphocytes in the blood and the levels of TNFα in serum. A statistically significant increase in serum TNFα was observed in persons exposed to chronic radiation exposure in the dose range from 0.08 to 4.23 Gy.
Russian Journal of Immunology. 2017;20(2):107-109
107-109
BLASTIC PLAZMATSITOIDS DENDRITOCELL TUMOUR: EXPERIENCE OF DIAGNOSTICS AND TREATMENT IN THE SVERDLOVSK REGIONAL ONCOHEMATOLOGICAL CENTER
摘要
Aim: to analyze the case of blastic plasmacytoid dendritic cell neoplasm (BPDN) detection and treatment in Sverdlovsk Regional Hematological Centre (Ekaterinburg). Bone marrow and peripheral blood samples obtained from female, aged 32, treated in Sverdlovsk Regional Hematological Centre during the Jan-Marth 2014. Diagnosis of BPDN verified using cytology, cytochemistry and immunophenotyping. Cytogenetic analysis was realized using G-banding. Detection of mutations in exons 12-13 ASXL1, exons 18-26 DNMT3A, exons 12-15 and 19-21 FLT3, exons 7-12 and 16-19 KIT, exons 1-4 KRAS, exons 9-12 NPM1, exons 1-4 NRAS, exons 4-11 ТР53, exons 6-9 WT1 genes was performed using direct sequencing. Cytological characterization of BPDN blast cells: large cells with oval, reniform nuclei with delicate chromatin structure, with one or two, at least - several small nucleoli, with wide basophilic cytoplasm and bright perinuclear area, richly vacuolated. In cytochemistry lipids is positive in 10.0 % of blasts, glycogen -6.0 %. The primary method of differential diagnosis of BPDN in case study was the identification of blast cells in the bone marrow. Immunophenotype of blast cells was CD4, CD15, CD33, CD38, CD56, CD64, CD65, HLA-DR(+), MPO-cyt (+/-). Karyotype was 48, XX, add(1)(p36), +6, +8 [8]. In case 2 gene mutations were co-existed in samples: KIT gene insertion and TP53 gene non-synonymous substitution с. 215 C>G. Coding sequence the remaining exons of the investigated genes is fully consistent with "wild type".
Russian Journal of Immunology. 2017;20(2):110-114
110-114
INFLUENCE OF THE BLOCKADE OF OPIOID RECEPTORS ON HUMORAL IMMUNITY IN MALE AND FEMALE MICE UNDER IMMOBILIZATION STRESS
摘要
It has been established that, both in females and in males, 6 h of immobilization resulted in a pronounced suppression of the amount of PFC in the spleen. Preliminary blockade of opiate receptors led to the abolition of the effect of stress in both males and females. However, in males, the administration of one naloxone had no effect on the number of PFC, while in females, naloxone inhibited the formation of PFC by both relative and absolute indices.
Russian Journal of Immunology. 2017;20(2):114-116
114-116
THE STATE OF ANTIMICROBIAL PROTECTION FACTORS OF NASAL SECRETION IN PATIENTS OPERATED ON THE CURVATURE OF THE NASAL SEPTUM IN THE EARLY POSTOPERATIVE PERIOD
摘要
The status of the functional-metabolic status of neutrophilic granulocytes (NG), interleukin (IL) -1β, IL-2, IL-8 in the nasal secretion of patients with 24-hour tamponade at 1,3 days after rhinosurgical intervention for curvature of the nasal septum was analyzed. The nasal secretion of the operated patients revealed: an increase in the content of neutrophilic granulocytes, an increase in the parameters of the neutrophilic granulocyte NGT- test, a decrease in the intensity of the phagocytic reaction intensity, a phagocytic number, an increase in IL-8, IL-2, and a decrease in IL-1-β increased by day 3.
Russian Journal of Immunology. 2017;20(2):117-119
117-119
PECULIARITIES OF IMMUNE REGULATION IN CHILDREN UNDER INDUSTRIAL EXPOSURE TO METALS
摘要
Changes of immune parameters in children under conditions of industrial metals contamination were associated with a decrease in phagocytic activity, inhibition of CD4 and CD95 expression and an augmentation in the number of CD25+ and CD127- lymphocytes, an increase in IgE content of total and specific IgG to aluminum, activation of synthesis of non-specific mediators of leukotrienes C4/D4/E4.
Russian Journal of Immunology. 2017;20(2):120-122
120-122
EXPERIMENTAL ESTIMATION OF CORRECTION OF HUMORAL IMMUNITY HEPATOPROTECTIVE DRUG HEPATOBIOL IN LABORATORY ANIMALS C ACUTE TOXIC HEPATITIS
摘要
The article is devoted to topical issues of preclinical study developed a combined experimental sample of a hepatoprotective drug heptobiol on humoral immunity in laboratory animals and reproduced with a model of acute toxic liver damage. Results showed that the manifestation of the inflammatory syndrome was more pronounced in the group of experimental animals without treatment is reproduced with toxic hepatitis. In group I, in which laboratory animals were assigned to drug heptobiol, the pathological process were less pronounced than in group II received the reference drug ursosan. It should be noted that at the end of the observation period (30th day), group I noted the normalization of all studied parameters, in contrast to group II, in which indicators of inflammatory syndrome were not recovered.
Russian Journal of Immunology. 2017;20(2):123-126
123-126
PRECLINICAL EVALUATION OF IMMUNOTROPIC ACTION OF PROBIOTICS BACILACK TRANSDERMAL THERAPEUTIC SYSTEM
摘要
The article is devoted to the study of immunotropic action developed bicomponent probiotic drug bacilact in a soft medicinal form (based on tizol, eftiderm and silicontitaniumorganical glycerohydrogel) on quantitative changes of parameters of cellular immunity in the treatment of localized infected wound animal skin, caused by Staphylococcus aureus. Thus, in particular, an increase in phagocytic activity of neutrophils of peripheral blood and peritoneal macrophages (average 2 times), increased the quantitative content of T - and B-lymphocytes (1.5-2 times). Studies to assess the immune status of experimental animals with localized purulent infection of the skin caused by Staphylococcus aureus, which treatment the subjects performed gel preparations, allows to make a conclusion about their pronounced immunomodulatory properties on parameters of cellular immunity. More significant effects observed for drugs on the basis of bacilact containing tizol and silicontitaniumorganical glycerohydrogel.
Russian Journal of Immunology. 2017;20(2):126-129
126-129
THE ROLE OF PREGNANCY-SPECITIC GLYCOPROTEIN IN THE REGULATION OF PRODUCTION OF KEY CYTOKINES THAT DETERMINE BALANCE Th1 / Th2
摘要
The effect of pregnancy-specific glycoprotein (PSG) on the production of Th1/Th2-cytokines by intact mononuclear cells and monocultures of CD4 cells under in vitro conditions by flow-through fluorimetry was studied. It has been established that PSG predominantly suppresses the production of Th1-cytokines (IFN-γ, TNF-α, IL-2) by the types of cells studied, and it regulates the production of Th2-cytokines in different directions, decreasing the production of IL-9, IL-13, IL-6, but increasing the level of IL-5. As a result, PSG forms the integral Th2-cytokine profile of intact peripheral cells of the immune system.
Russian Journal of Immunology. 2017;20(2):129-131
129-131
LOCOMOTORY REACTIONS OF PHAGOCYTES AT DIABETEA
摘要
Diabetes remains an urgent problem. According to WHO data the number of patients with Diabetum makes 3-5 %. The risk of development of postoperative infectious complications in patients with Diabe-tum is 5 times higher, than at patients without it. The research of hemotaksichesky activity of phagocytes in relation to a glucose and insulin is conducted. The revealed changes of locomotory reactions of phagocytes can demonstrate the blockade of the receptor device of neutrophils and monocytes developing in the conditions of a hyperglycemia.
Russian Journal of Immunology. 2017;20(2):132-133
132-133
STAPHYLOCOCCUS AS PRODUCERS OF CYTOKINE-LIKE SUBSTANCES
摘要
In experiments in vitro on the 23 clinical isolates S. aureus (n=23) a test-system Multiplex (Luminex, USA) to determine the 15 cytokines (G-CSF, GM-CSF, INF-γ, IL-10, IL-12p70, IL-13, IL-17A, IL-β, IL-2, IL-4, IL-5, IL-6, MCP-1, MIP-1β, TGF-α) and test-system studied to determine the 27 cytokines (BasicFGF, Eotaxin, G-CSF, GM-CSF, INF-γ, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IP-10, MCP-1, MIP-1a, MIP-β, PDGF-BB, RANTES, TNF-α, VEGF) the production of cytokine-like substances (CLS). Identified and characterized the intergeneric and intraspecific variability of S. aureus in their ability to produce CLS in the environment of cultivation. It is established that isolates of S. aureus are the most active producers of CLS. The possible pathogenetic significance of products CLS S. aureus and ways to further study this phenomenon were discussed.
Russian Journal of Immunology. 2017;20(2):134-136
134-136
SYNTHETIC PEPTID THE ACTIVE CENTRE OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR (GM-CSF) RANGE OF ITS IMMUNOBIOLOGY ACTIVITY AND PRACTICAL APPLICATION
摘要
In experiments in vitro and in vivo it was shown that a synthetic peptide of the active site of GM-CSF -ZP2 (chemical formula: THR NLE NLE ALA SER HIS TYR LYS GLN HIS CYS PRO) stimulates the activity of immune system (lymphocytes, stem cells, neutrophils, monocytes), inhibits the growth, reproduction and biofilm formation of gram-positive and gram-negative bacteria and has antiviral activity against DNA and RNA viruses and activates a reparation of wounds. The presence at this synthetic peptide of such a wide range of immunological effects (immunostimulatory, antibacterial, antiviral, and repair activity) determines the possibility of its use as the basis for creation of new effective medicinal and cosmetic preparations with combined properties that was realized at creation of ACEGRAM.
Russian Journal of Immunology. 2017;20(2):137-140
137-140
IMMUNOGENETICAL FEATURES AT VIOLATIONS OF REPRODUCTIVE FUNCTION IN WOMEN EXPOSED TO PHENOLS
摘要
The polymorphic variants of candidate genes associated with immune system, violation of the women reproductive function, as well as with the metabolism of phenols have been analyzed. The study revealed that in the surveyed group, marked by high content of phenols in the blood, the higher frequencies of heterozygous genotypes of VEGF G634C, PPARD A/G, PPARA G2528C, variant homozygous genotypes of TERT C309G and PPARGC1A G1444A genes were detected. It has been noted, that the gene PPARD A/G (p<0.05) characterizes the features in women of this cohort survey. The analysis of the "hapten-genotype" systems, showed an association between the level of phenol, o- and p-cresol, and genotypes of genes: MTHFR C677T, PPARD A/G, ESR1 G2014A. The studied genes can be used as markers of sensitivity that indicates on the preconditions to the development of immunemediated disorders of reproductive function in women induced by an increased content of phenols in blood.
Russian Journal of Immunology. 2017;20(2):141-143
141-143
IgAs, IL-6 AND IFN-γ IN NASAL FLIUD IN TRANSIENT HYPOGAMMAGLOBULINEMIA OF INFANCY
摘要
To evaluate IgAs, IL-6 and IFN-γ in nasal fluid in transient hypogammaglobulinemia of infancy (THI) 15 children at the age 6 months to 4 years with THI, 30 children with recurrent respiratory infections and 16 healthy persons at the same age were studied. There has been registered a decrease in IgAs and IFN-γ and an increase in IL-6 in THI as compared to healthy children and sufferers of recurrent respiratory infections with no THI. Received data may be interesting for our understanding immunoregulation of antibody synthesis and inflammation in THI.
Russian Journal of Immunology. 2017;20(2):144-145
144-145
CROSS-LINKING ALLERGY IN BRONCHIAL ASTMA AND ALLERGEN-ASSOCIATED MOLECULAR PATTERNS (AAMP)
摘要
To evaluate content of IL-4 in lymphoblast transformation test (LTT) supernatants of 15 persons with asthma, 16 patients with asthma in association with allergy to prawns and 15 healthy persons were studied. All sufferers of asthma were sensibilisized to Der p 1 (Dermatophagoides pteronissinus). There have not been revealed any differences between the IL-4 in different groups that may be indirectly propose the presence of Allergen-Associated Molecular Patterns (AAMPs) in patients, which might smooth out received results.
Russian Journal of Immunology. 2017;20(2):146-147
146-147
THE INTERACTION OF IMMUNE CELLS OF THE SKIN IN THE PROCESS OF REPARATIVE REGENERATION IN THE WOUND
摘要
An experiment is conducted on 20 adult rabbits in order to clarify the role of immune cells in the reparative regeneration of skin in conditions of purulent-infected wounds. Understanding the role of immune cells in the regeneration processes in the wound and the formation of scar tissue may may contribute to search for new approaches to therapy-related effects on the immune mechanisms for the prevention of scarring.
Russian Journal of Immunology. 2017;20(2):148-150
148-150
PERIPHERAL BLOOD B CELL SUBSETS IN PATIENTS WITH MULTIPLE SCLEROSIS
摘要
Demyelination during multiple sclerosis (MS) can be associated with B-cells that can produce au-toreactive antibodies and can be localized in specialized lymphoid follicles within the central nervous system. Using multicolor flow cytometry, it was found out that the relative number of peripheral blood CD19+ B cells in patients with MS (n = 25) was significantly higher (p = 0.005) when compared with the healthy volunteers (n = 27, 17.88 % (12.88, 25.15) and 11.77 % (9.52, 15.26), respectively). To identify the distinct B lymphocytes subsets we studied coexpression of IgD and CD38, IgD and CD27, as well as CD5 and CD27. It was shown that in patients with MS the relative number of IgDdimCD38low "naive" and IgDlow memory subsets was significantly decreased due to the increased proportion of activated IgDdimCD38dim B lymphocytes. Furthermore, the relative content of B cells with the IgDdimCD27low phenotype was significantly increased (p < 0.001), while the percentages of the other subsets purified on the co-expression of IgD and CD27 were significantly reduced. A detailed analysis of B cells and follicular T-helpers - Th subset that affects the maturation and differentiation of B lymphocytes - could have a significant impact on the current understanding of the causes and pathogenesis of MS, as well as could develop the new approaches to the therapy of this disease.
Russian Journal of Immunology. 2017;20(2):150-154
150-154
INFLUENCE OF "TSITOVIRA-3" ON THE EXPRESSION OF IL-8 AND ICAM-1MONONUCLEAR LEUKOCYTES OF PERIPHERAL BLOOD OF THE PERSON AND CAGES OF THE EA.HY926 INVITRO LINE
摘要
The aim of the current study was to investigate cell mechanisms by means of which the "Cytovir-3", the drug for prevention and treatment of the influenza and acute respiratory viral infection, exerts its pharmacological effects. As a cellular model, an endothelial EA.hy926 as well as mononuclear leukocytes (ML) of human peripheral blood were chosen. The effects of co-incubation of "Cytovir-3" and cell lines on IL-8 and ICAM-1 expression were evaluated. In the presence of "Cytovir-3" at concentration of 10.0 ng/ml and 1.0 ng/ml, we found a significant increase in spontaneous secretion of IL-8 by endothelial cells by 10 % and 15 % accordingly. Besides, at concentration of 10.0 ng/ml the investigating drug significantly enhanced the expression level of ICAM-1 induced by TNFa. Revealed stimulating action of "Cytovir-3" on endothelial cells and ML can suggest its influence on vascular component of inflammatory reaction.
Russian Journal of Immunology. 2017;20(2):155-156
155-156
INFLUENCE OF GLUTAMIL-TRIPTOFAN ON THE EXPRESSION OF IL-8 AND ICAM-1 MONONUCLEAR LEUKOCYTES OF PERIPHERAL BLOOD OF THE PERSON AND CAGES OF THE EA.HY 926 LINE
摘要
The aim of the study was to investigate the cell mechanisms mediating immunomodulatory effects of a-glutamyl-tryptophan (EW) and estimate the possibility of this pharmaceutical agent to impact the level of NF-kp phosphorylation. Using in vitro model of inflammation, we tested whether EW impacts spontaneous and TNFa induced IL-8 secretion as well as ICAM-1 expression by endothelial EA.hy 926 cells and mononuclear leukocytes of human peripheral blood. Revealed inhibition of pro-inflammatory cytokine production can contribute to anti-inflammatory effects of EW. However a capability of EW to increase ICAM-1 expression suggests its cell activating function. Thus, due to diverse effects it seems unlikely that EW influences NF-kp directly.
Russian Journal of Immunology. 2017;20(2):157-158
157-158
EVALUATION OF INTRAVITREAL RANIBIZUMAB INJECTION INFLUENCE ON TEAR FLUID IMMUNE STATUS IN PATIENTS WITH AGE-RELATED MACULAR DEGENERATION
摘要
Influence of intravitreal injection of vascular endothelial growth factor A inhibitor (VEGF-A) for aged-related macular degeneration treatment on tear fluid immune status, which was evaluated by multiplex analysis with assay of BasicFGF, Eotaxin, G-CSF, GM-CSF, INF-γ, IL-10, IL-12p70, IL-13, IL-15, IL-17A, IL-1β, IL-1ra, IL- 2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IP-10, MCP-1, MIP-1α, MIP-1β, PDGF-BB, RANTES, TNF- a, VEGF was studied. The study showed that given treatment result in a significant decrease in BasicFGF, Eotaxin, IL-13, TNF-α levels in tear fluid in one month.
Russian Journal of Immunology. 2017;20(2):159-161
159-161
NANO AND MICRODIMENSIONAL PARTICLES - A NEW LINK IN THE ETIOLOGY OF PERIIMPLANTITA
摘要
At present, it is being assumed that developments of inflammatory complications accompanying long-term functioning of dental implants in the patient's mouth, are correlated with the development of such diseases as mucositis and peri-implantitis. The pathogenesis of these dental implant complications remains, in our opinion, unexplored in terms of the role of immunological component in the mechanism of acute and chronic inflammation, which in turn requires research aimed at identifying the true causes, which may be proven biologically. It is being observed that, with standardized manufacturing techniques in use when it comes to the practical use of dental implants themselves, the clinical picture of their long-term use is personalized, which in turn can manifest in diminution of bone tissue and require surgical revision or reimplantation. The main causes of these inflammatory complications are correlated with personalized bacterial microenvironment, forming a biofilm on the surface of the dental implant in the patient's mouth, as well as with inadequate distribution of functional pressure connected with the manufacturing and use of the prosthesis. The role of emission of nano and microsized metal particles distributed in the oxide layer of the dental implants manufactured based on the TiO2 alloy and their involvement in long-term interstitial interaction, has not been studied yet. This fact makes it necessary to conduct scientific studies on the immunological aspects of tissue repair at the cellular and molecular levels, and opens up a new perspective on the study of results when it comes to personification of dental implantation.
Russian Journal of Immunology. 2017;20(2):162-165
162-165
MICROBIOLOGICAL AND IMMUNOLOGICAL ASPECTS OF THE INTERACTION OF PSEUDOMONASAERUGINOSA,STAPHYLOCOCCUSAUREUS AND ESCHERICHIACOLI WITH NANOSCALE METAL PARTICLES, RECIEVED FROM THE SURFACE OF DENTAL IMPLANTS
摘要
The purpose of this study was to investigate the interaction of nanosized metal particles obtained in the laboratory from the surface of Nobel Biocare dental implants with Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli in the presence of native and heat inactivated human serum. In vitro experiments it was established that when nanoparticles were added to test samples of native sera, the accumulated biomass of S. aureus and E. coli was increased by 8.1-8.3 % in broth culture and P aeruginosa by 42.9 % in Comparison of control, indicating that they inhibited the bactericidal effect of the complement system. In addition, the potentiating effect of native serum on biofilm formation of bacteria on the surface of nanoparticles was recorded. Possible immunological consequences of interaction in the system "nanoparticle-whey protein-bacteria in the process of osseointegration" are discussed.
Russian Journal of Immunology. 2017;20(2):166-169
166-169
THE INDICES OF IMMUNITY AND THE CLINICAL COURSE OF POSTTRAUMATIC PERIOD IN PATIENTS WITH PENETRATING WOUNDS OF THE EYEBALL WHEN INCLUDED IN A COMPREHENSIVE TREATMENT OF THE DRUG BESTIM
摘要
We conducted a study of indicators of systemic immunity and the clinical course of post-traumatic period in 38 patients with penetrating wounds of the eyeball severe. In the first group 20 patients received pathogenetic basic treatment, in the second group 18 patients in the standard treatment included intramuscular injection of immunomodulator Bestim 100 mcg daily for 5 days, starting 3 days after the injury. We studied subpopulation composition of lymphocytes in the blood serum on 2-3 and 10 days after injury, the timing of the relief of the inflammatory symptoms, the clinical course of posttraumatic period at 14 and 30 days after injury. It was established that the inclusion of the immunomodulator Bestim in the basic treatment of the PWYB led to the increase in the absolute number of B2- and the amount of B-lymphocytes, the absolute number of CD4+CD127+CD25- and T-activated lymphocytes, a decrease in the relative number of B1-lymphocytes and lack of fall of the level of T-regulatory cells, which have a positive impact on the clinical course of posttraumatic period in patients of the 2nd group: in 14-th and 30-th day it has increased frequency of relief of inflammatory symptoms as in the group as a whole as per patient and per recovery by 1 month after injury.
Russian Journal of Immunology. 2017;20(2):169-173
169-173
THE FEATURES OF IMMUNOLOGICAL REACTIVITY OF WOMEN YAMALO-NENETS AUTONOMOUS DISTRICT
摘要
Conducted the expedition in the town of Nadym in Yamalo-Nenets Autonomous district (April, 2015). We examined 30 women, aged 40-64 living in the city of Nadym in Yamalo-Nenets Autonomous district. Immunological studies conducted in the laboratory of physiology of immunocompetent cells of the Institute of physiology of natural adaptations, VICKIE RAS, Arkhangelsk. In the peripheral blood were determined: the concentration of the phenotypes of lymphocytes CD16+, CD25+, CD71+, CD95+, HLADR+, the total number of leukocytes. The surveyed women in the city of Nadym in Yamalo-Nenets Autonomous district finds a reduction in the reserve capacity of the immune homeostasis due to the increased cytotoxic activity (CD16+) and apoptosis (CD95+) 56,66±2,49 and of 36,66±2,00 %, respectively.
Russian Journal of Immunology. 2017;20(2):174-176
174-176
INFLUENCES OF HEXAPEPTIDE ARGINYL-ALPHA-ASPARTIL-LIZIL-VALYL-TYROSIL-ARGININ ON THE TRANSFORMED PHENOTYPE OF NEUTROPHILIC GRANULOTSITES OF HEALTHY PERSONS IN THE EXPERIMENTAL SYSTEM INVITRO
摘要
The experimental model of the in vitro transformed phenotype of neutrophilic granulocytes (NG) equipped with receptors CD64, CD32, CD16, CD11b, was created under the influence of fMLPs. The features of the transformed phenotype of NG were described. The influence of the Hexapeptide arginyl-alpha-aspartyl-lysyl-poured-tyrosyl-arginine (HP) on untransformed and transformed phenotype of NG was investigated in vitro. It was shown that fMLP increases the number of NG, expressing the receptor CD64, increases the density of expression of the receptor CD11b, and moderately reduces the amount of CD32+ NG. HP does not influence on the number of NG, bearing receptors CD64, CD32, CD16, CD11b, and the level of their expression. At the same time with simultaneous incubation with fMLP NG and HP, are shown to mitigate the effects of fMLP: reduced the number of CD64+ NG, reduced the density of CD11b expression, decreases in the number of NG bearing a receptor CD32. Overall, the results demonstrate the classic immunomodulatory effect HP: on the one hand, the absence of any change in the studied receptors of NG under his influence and leveling the transformational changes of phenotypic characteristics of NG, which arose under the influence of fMLP.
Russian Journal of Immunology. 2017;20(2):176-179
176-179
THE ROLE OF THE MONOCYTIC AND MACROPHAGAL SYSTEM IN THE DYNAMICS OF THE REPLACEMENT REGENERATION IN CASE OF THE ELASTIN BIOMATERIAL TRANSPLANTATION
摘要
The article contains the results of the morphological investigations when subcutaneously transplanting two types of the elastin biomaterial: structurized and dispersed one (patent № 2440148). The experiments have been carried out on Wistar rats (n=59). The transplanted biomaterials were subjected to the stage-by-stage resorption with further formation of the connective tissue regenerate. When doing it, the cells of the macrophagal and fibroblastic differentiation come out as a unified morphofunctional system in which giant multinuclear cells are an indispensable element. Depending on the biomaterial structure there are singled out two histotopographic types of the replacement regeneration: frontal type for the structurized biomaterial and diffuse-locunar one for the dispersed biomaterial.
Russian Journal of Immunology. 2017;20(2):179-182
179-182
EFFECT OF ERYTHROPOIETIN ON FUNCTIONAL ACTIVITY OF BLOOD NEUTROPHILS OF PREMATURE INFANTS IN EXPERIMENT INVITRO
摘要
Aim of the study - to investigate the effect of erythropoietin (EPO) on the absorption and NBT-re-ducing capacity of neutrophils isolated from the blood of preterm neonates (PN) in vitro. The study was performed on 18 infants: PN from premature birth (PB) (n=6); PN from early preterm birth (EPB) (n=6); PN from a very early preterm birth (VEPB) (n=6). The EPO used in concentrations of 30 IU/l; 15 IU/l; 7,5 IU/l; 3,75 IU/L. Evaluated the absorption capacity and oxygen-dependent metabolism of neutrophils isolated from umbilical cord blood. Have PN from EPB and VEPB, EPO in in vitro experimental conditions increases, and PN from PB - reduces the absorption capacity and NBT-reducing capacity of blood neutrophils. EPO effect on the absorption capacity of blood neutrophils in in vitro experimental conditions does not depend on gestational age and manifests itself in small doses (3,75 IU/l and 7.5 IU/l), and NBT-reducing capacity of blood neutrophils in large doses (15 IU/l and 30 IU/l).
Russian Journal of Immunology. 2017;20(2):182-185
182-185
THE EFFECT OF LOCAL APPLICATION OF ERYTHROPOIETIN ON DEATH INDICATORS OF LYMPHOCYTES IN THE BLOOD IN THE DYNAMICS OF EXPERIMENTAL THERMAL INJURY
摘要
To investigate the effect of local application of erythropoietin (EPO) in the composition of transdermal film (TDF) on the death's indicators of lymphocytes in the blood in dynamics of experimental thermal trauma (TI). The experiment was made out on 90 white nonlinear rats that were divided into groups: 1 - intact rats; 2 - animals with the TI and an aseptic bandage (n=40), 3 - animals with the TI application of the TDF with EPO (n=40). Modeling TI IIIA degree and area of 3.5 % was carried out by immersing the interscapular region of the skin of the animal in clean water with a temperature of 98-99°C 12 s. In the preliminary studies was developed TDP on the basis of sodium carboxymethyl cellulose with EPO at the dose of 500 IU/g. The dressing was carried out daily. The stady was conducted for 3rd, 5th, 8th and 14th days. Death's indicators of lymphocytes was assessed by staining cells with fluorochrome conjugated with annexin V and 7-aminoantipyrine D in flow cytometer "Navios". It is established that at TI at 3, 5 and 8 day increase in the number of lymphocytes with the phenotype of the Annexin-5-FITC+/7-AAD- and Annexin-5-FITC+/7-AAD+, 5 and 8 day reduced the number of lymphocytes with the phenotype of the Annexin-5-FITC-/7-AAD-. In the application of TDF with the EPO at the TI for 5 and 14 days reduced the number of lymphocytes with the phenotype of the Annexin-5-FITC+/7-AAD-, 8 and 14 days, reduced the number of Annexin-5-FITC+/7-AAD+ cells at 5 and 14 days increased lymphocytes Annexin-5-FITC-/7-AAD-. Thus, in TT the application of TDP with EPO leads to a decrease in blood lymphocytes with signs of apoptosis and necrosis.
Russian Journal of Immunology. 2017;20(2):185-188
185-188
CHANGES OF IMMUNE REGULATORY INDICATORS AT CHILDREN IN THE CONDITIONS OF AN EXPOSITION STRONTIUM
摘要
The analysis of marker indicators of immune regulation at children in the conditions of excess intake of strontium with drinking water is carried out. Research showed that markers of specific hypersensitivity and mediators of intercellular immune regulation: IgG specific to strontium, cytokins of IL-6, IL-12, IL-17, α-TNF, LTC4/D4/E4 - spontaneous and specifically stimulated, and osteoprotegerin can be used by RANKL for an assessment of risk of violation of health as early markers of changes of immunoregulation at the children living in exposition conditions strontium.
Russian Journal of Immunology. 2017;20(2):188-191
188-191
THE LEVEL OF SPONTANEOUS AND ANTI-СD3-STIMULATED СD38 EXPRESSION ON T-LYMPHOCYTES IN CHILDREN WITH AUTOIMMUNE PATHOLOGY
摘要
Children of 6-17 years old with systemic lupus erithematosus (n=10), with systemic sclerosis (n=10), with juvenile idiopathic arthritis (n=47) and conditionally healthy controls of corresponding age (n=30) were examined. It was revealed by flow cytometry that after 24-hours incubation (37 °C, 5 % СО2) the spontaneous and anti-СD3-stimulated amount of СD38-positive T-lymphocytes, T-helpers and cytotoxic T-cells in children with rheumatic diseases was decreased in comparison with healthy control.
Russian Journal of Immunology. 2017;20(2):191-194
191-194
HUMORAL IMMUNOLOGICAL PARAMETERS IN CHILDREN WITH JUVENILE IDIOPATHIC ARTHRITIS AND REACTIVE ARTHRITIS
摘要
The levels of humoral immunological parameters in children of 2-17 years old with oligoarticular juvenile idiopathic arthritis (n = 98), with reactive arthritis (n = 26) and conditionally healthy children (n = 42) were examined. There were no differences in the concentration of C-reactive protein and antibodies to modified citrullinated vimentin between healthy children and patients with joint diseases. The level of antibodies to extractable nuclear antigens and the antinuclear factor titer in children with juvenile arthritis were higher, than in healthy children and patients with reactive arthritis. The frequency of antibodies to extractable nuclear antigens and modified citrullinated vimentin in children with juvenile arthritis and reactive arthritis was low, most often an antinuclear factor in these patients was detected.
Russian Journal of Immunology. 2017;20(2):194-197
194-197
KLINIKO-LABORATORI EFFICIENCY OF GLUTAMIL-TRYPTOPHANE IN THERAPY OF THE HOSPITAL PNEUMONIA PROCEEDING AGAINST THE DEFICIENCY OF BODY WEIGHT (DBW)
摘要
The clinical laboratory efficacy of the drug "Timogen®" (glutamyl-tryptophan) in the treatment of hospital pneumonia in patients with weight deficit was evaluated. Eighty-four patients with hospital pneumonia and objective signs of weight deficit were examined. Patients were randomized into two groups: 40 people received standard etiopathogenetic therapy and 44 patients used "Timogen®" metered dose nasal spray as a supplement to standard therapy. The drug was administered in the acute period of the disease in a daily dose of 100 mcg a 7-day course. The application of the "Timogen®" was accompanied by statistically and clinically significant reduction in the duration of the main clinical signs of the underlying disease, normalization of structural and functional parameters of the immune system and restoration of nutritional status.
Russian Journal of Immunology. 2017;20(2):197-199
197-199
TREATMENT-AND-PROPHYLACTIC EFFICIENCY OF SYNTHETIC PEPTIDE OF GLUTAMIL-TRYPTOPHANE AT THE VISCERAL PATHOLOGY CAUSED BY A HEAVY MECHANICAL POLYTRAUMA
摘要
The efficacy and safety of using synthetic dipeptide alpha-glutamyl-tryptophan in the complex therapy of severe mechanical polytrauma of various nature were investigated. The trial included 327 people. Patients were randomly assigned to two groups: experimental and control (160 and 167 people, respectively). Both groups received standard combination therapy, but the experimental group received additionally the immunotropic drug "Timogen®" (alpha-glutamyl-tryptophan). The drug was administered intramuscularly in a daily dose of 100 mcg during 10-day course not later than 24 hours after traumatic injuries. The structure, the severity and the progress of the visceral pathology, as well as dynamics of the immune system were evaluated in the comparative groups. "Timogen®" facilitates reduction of the incidence of visceral complications, and if they develop, it decreases duration of the main clinical manifestations normalizing the structural, quantitative and functional parameters of the immune system and accelerating regeneration of damaged tissues in patients with severe mechanical polytrauma.
Russian Journal of Immunology. 2017;20(2):199-201
199-201
THE LOCAL IMMUNE RESPONSE IN PATIENTS AT EARLY POST-SURGERY PERIOD AFTER DENTAL IMPLANTATION DEPENDING ON THE SCHEMES OF ANTIBACTERIAL TREATMENT
摘要
Successful performing of dental implantation is one of the major aims of dentistry. Surgery induces violation of the integrity of the mucosa, activation of the mucosal immunity, reparation and regulation processes, that can be measured by levels of IgM, IgG, sIgA and also IL-φ, IL-4, IL-6, IL-10, IL-17A, IFN-γ, TNF-α on the local or systemic level. Some antibiotics and phytotherapy can be applied to prevent potential onset of postoperative complications. Differences in its efficiency and action on the mucosal immunity can be measured with comparative assay of the features of the local immunity in the saliva of patients before dental implantation and after applying the therapies. Application of antibiotics leads to decreasing activity of pathogenic microorganisms and reparative potential of the mucosa cells, that is manifested in statistically significant reduction of the IgG and increased IgM and sIgM levels, whereas phytotherapy enhances activity of the immune system due to stimulation of marked increase in the level of IL-4, IL-10, IFN-γ and TNF-α in the patients' saliva.
Russian Journal of Immunology. 2017;20(2):201-207
201-207
FUNCTIONALIZATION OF CARBON NANOPARTICLES WITH ANTI-IGE APTAMER
摘要
Technology of conjugation of carbon nanoparticles with DNA aptamer based on biotin-streptavidin interaction was developed and optimized. Sorption of 30-60 pM DNA per mg of nanoparticles was obtained at the initial ration of 125-500 pM/mg.
Russian Journal of Immunology. 2017;20(2):207-211
207-211
THE IMPACT OF THE AMINO ACID ARGININE ON THE COURSE OF EXPERIMENTAL TUBERCULOUS PROCESS IN ANIMALS WITH DIFFERENT SENSITIVITY TO TUBERCULOSIS
摘要
On the model of experimental tuberculosis of guinea pigs to study the effect of amino acid arginine on the course of the tuberculosis process. Experiments were carried out on 75 guinea pigs and 25 rats (weighing 200-250 g). To reproduce experimental tuberculosis, a museum strain of mycobacteria was used at the Central Research Institute of Tuberculosis H37Rv, 1993. Groups of animals: 3 groups of guinea pigs of 25 pcs. (Healthy control, infected with ILW and infected with ILT, receiving arginine daily 10 mg / kg body weight of the animal for a month) and 2 groups of rats of 25 pieces (healthy control, infected with ILW and infected with MBT). Determined in the blood nitrites and nitrates, total lipid peroxides by ELISA Biomedica GRUPPE and R & D Systems. Results. In animals lacking resistance to tuberculosis infection, increased plasma levels of nitric oxide derivatives caused by the use of arginine is an unfavorable factor contributing to the progression of tuberculosis.
Russian Journal of Immunology. 2017;20(2):212-214
212-214
PARTICIPATION OF PLASMA FACTORS OF FOLDING IN THE COURSE OF FORMATION THE EKSTRATSELLYULYAR OF NETWORKS NEUTROPHILS
摘要
The article presents the results of the assessment of the role of dotting factors FVII, FV, FII in formation of extracellular traps by neutrophils.
Russian Journal of Immunology. 2017;20(2):215-217
215-217
FEATURES OF THE IMMUNE STATUS OF ATHLETES OF ACYCLIC SPORTS (FIGHTERS AND BOXERS) DEPENDING ON THEIR SPORTS QUALIFICATION
摘要
The study of blood cell counts and analysis of their changes in wrestlers and boxers made it possible to reveal special features of the population and subpopulation spectrum of lymphocytes in wrestlers and boxers of different qualifications. The sportsmen of power sports with anaerobic metabolism (wrestlers and boxers a significant decrease in the general population of T-lymphocytes) occured due to a decrease in circulation of main subpopulations of T cells: T-helper and T-cytotoxic. There were also a decrease in number of cells with adhesion receptors (CD11b), the number of B-lymphocytes and cells bearing apop-tosis readiness markers (CD95), opposite to elevated cell count with lymphocytes early positive activation(CD25) markers. An increase in cell number with receptors for interleukin-2 (CD25) with parallel decrease in the number of hematopoietic cell precursors and the main subpopulations of lymphocytes (T, B lymphocytes) is considered to be a reflection of the negative impact of intense speed-strength anaerobic regimen on immune system, and possible may be resulted in immunodeficiency in these athletes. The immune status of athletes going in for acyclic sports is characterized by marked cytopenia of relative and absolute numbers of T-lymphocytes, both T-helper and T-cytotoxic lymphocytes, and also CD16 and CD56 positive cells, belonging to congenital immunity factors, and also B lymphocytes (CD20), compared to sportmen groups of skiers and swimmers, and that reflects a decrease in intensity of immunopoiesis in anaerobic type of energy metabolism, and considered to be a significant factor, affecting immune system quality reaction and T- and B-cell cooperation reaction.
Russian Journal of Immunology. 2017;20(2):217-220
217-220
FEATURES OF PARAMETERS OF IMMUNE SYSTEM DEPENDING ON THE SPORT
摘要
Immunological study of peripheral blood cells with subsequent analysis of data obtained during examination of professional skiers and swimmers, that meet presumably aerobic specific physical activity conditions, comparing to data obtained from professional wrestlers and boxers, that meet presumably anaerobic specific physical activity conditions, shows some special features in peripheral blood cell count, namely, higher leucocyte count and eosinophil content in skiers and swimmers, comparing to wrestlers and boxers, and lower content of mature neutrophil, but higher content of early neutrophilic cell forms in wrestlers and boxers, accompanied by evident decrease in mature lymphocyte and monocyte content in skiers and swimmers, comparing to wrestlers and boxers. A significant decrease in absolute and relative content of T-lymphocytes and both T-helper and T-cytotoxic lymphoid subpopulations, as well as CD16 и CD56 -positive cells and CD20 B-lymphocytes in anaerobic sportsmen were observed, comparing to those in aerobic sportsmen, that was due to immunopoiesis decrease in anaerobic specific physical activity conditions. Cell activation markers investigation led us to conclusion of favorable signs of current adaptation processes in cyclic sportsmen, derived from higher absolute B-cell early precursors content and higher CD20 mature B-cell content observed, and was considered to be B-lymphopoiesis augmentation in aerobic sportsmen. The groups mentioned above also reveal a rise in absolute amount of peripheral blood cells, possessing cell-cell and endothelial cell -adhesion receptors, as well as CD95 receptor possessing immune cells, involved in FAS-mediated cell apoptosis, and also a rise in early CD25 and late HLA-DR- activation receptors-possessing cell subpopulations, and rise in CD34 leucocyte precursor cell subpopulations, that demonstrate high capacity of immune cells to cooperate with each other in multisystem cooperation, that leads to greater adaptation potentials of immune system in aerobic sportsmen. In groups of wrestlers and boxers a twofold levels of secreted IgA were observed, and was associated with a decrease in secondary immune response-associated IgG level.
Russian Journal of Immunology. 2017;20(2):221-223
221-223
TREG CELLS AND INFLAMMATORY DISEASES
摘要
This work highlights the flow cytometry data about the level of several regulatory T cell (Treg) subpopulations in inflammatory diseases: acute pancreatitis and rheumatoid arthritis.
Russian Journal of Immunology. 2017;20(2):224-226
224-226
CLINICAL AND LABORATORY EVALUATION OF COMBINED APPLICATION OF ADAMANTANES AND ETIOPATHOGENETIC DRUGS IN INFLUENZA AND ACUTE RESPIRATORY VIRAL INFECTIONS IN CHILDREN
摘要
The clinical and laboratory efficacy and safety of combined use of the antiviral pharmaceutical product (Adamantine family) and the complex preparation for etiopathogenetic therapy of influenza and ARVI in children were examined in the prospective clinical trial. The trial involved 90 randomized into 2 groups children aged 2-6 years with verified diagnoses of influenza and ARVI. Patients of the first group received the drug "Citovir®-3", children of the second group received combined therapy ("Citovir®-3" + "Orvirem®"). Evaluation of the efficacy and safety of the investigational drugs was performed by means of comparative intergroup statistical analysis of generally accepted for such nosology clinical signs and laboratory findings, frequency and severity of adverse events. The analysis of the results revealed that the use of "Citovir®-3" as a monotherapy and in combination with "Orvirem®" contributes to the reduction of the main manifestations of infectious process without statistically significant intergroup differences. Monotherapy with "Citovir®-3" leaded to a more pronounced increase in the level of secretory immunoglobulin A in the entrance gate of infection (saliva) and α-interferon in the serum. During the clinical trial no adverse events and abnormal laboratory findings were found in the comparative groups. The efficacy and safety of combined use of "Citovir®-3" and "Orvirem®" allow us to recommend this combination for treatment of verified influenza A in children aged 2-6 years. For the treatment of ARVI of another etiology, the combined use of the investigational drugs has no advantage as compared to the monotherapy with "Citovir®-3".
Russian Journal of Immunology. 2017;20(2):227-229
227-229
COMPARATIVE CLINICAL AND LABORATORY EVALUATION OF VARIOUS DRUGS FOR ETIOPATHOGENETIC THERAPY IN INFLUENZA AND ACUTE RESPIRATORY VIRAL INFECTIONS
摘要
The comparative clinical efficacy and safety of drugs "Citovir®-3" powder for oral solution, "Citovir®-3" syrup and "Immunal®" oral solution in the treatment of influenza and ARVI in children were examined in the prospective clinical trial. Ninety children aged 2 to 6 years with influenza and acute respiratory viral infections of unknown etiology were included in the trial in accordance with inclusion criteria. All patients were randomized into 3 groups, each of which received one of the comparative drugs as a means of etiopathogenetic therapy. The investigational drugs were used in the age-specific dosage variances as outlined in patient information leaflet. The analysis of the obtained results showed that the efficacy of the investigational dosage forms of the "Citovir®-3" (powder and syrup) against influenza and other ARVI exceeded the efficacy of the comparative drug "Immunal®" in the speed of normalization of clinical and laboratory parameters. The "Citovir®-3" (powder and syrup) contributes to statistically significant and clinically meaningful reduction in the duration of the febrile period and some catarrhal symptoms in comparison with "Immunal®" in influenza and ARVI. "Citovir®-3" (powder and syrup) facilitates the raise of secretory IgA production in the nasopharynx. All the investigational drugs were characterized as well tolerated. No adverse events or adverse drug reactions were found during the trial. Given the clinical and laboratory efficacy and safety profile of "Citovir®-3" (powder and syrup) they can be recommended for their inclusion in the treatment of children with influenza and ARVI.
Russian Journal of Immunology. 2017;20(2):230-231
230-231
EXPERIMENTAL AND THEORETICAL STUDY MECHANISMS OF THE IDIOTYPIC REGULATION OF AUTOIMMUNE REACTION INDUCED BY ALLOANTIGEN
摘要
Injection of allogeneic erythrocytes to mice leads to the development of anemia, the appearance of antibodies specific to RE, whose production coincides with the production of anti-erythrocyte autoantibodies and are characterized by progressive growth. The results of computer studies have been shown that the similarities between theoretical and experimental kinetics were achieved by applying a signal to the clone located as far from the autoreactive clone as possible in the investigated fragment of the immune network. Herewith, all clones of the immune network fragment demonstrate a progressive, continual growth of activity, that indicates a breakdown in the state of tolerance. Thus, the emergence of systemic chronic autoimmune processes may be the result of an immune response to antigens that activate lymphocytes, which specificity is similar but not identical to the specificity of lymphocytes recognizing autoantigens.
Russian Journal of Immunology. 2017;20(2):234-235
234-235
EFFECT OF THE AUTOLOGOUS APOPTOTIC CELL CULTURES ON THE PARAMETERS OF EARLY AND LATE APOPTOSIS OF T LYMPHOCYTES FROM HEALTHY DONORS
摘要
In healthy people, it has been established the possibility of induction of early and late apoptosis of T-lym-phocytes proliferated in vitro under physiological conditions through the transfer of both the cellular and humoral components of an autologous apoptotic T cell culture. It was determined that under equal conditions of cultivation of lymphocytes: a high cell density and a depletion of the culture medium, the transfer of components of the cells stimulated by anti-CD3 antibodies regularly increased the sensitivity of T-lymphocytes to apoptosis. While transfer of components of the culture with the dexamethasone exerted a modulating effect.
Russian Journal of Immunology. 2017;20(2):236-238
236-238
LYSOZYME OF SALIVA AND ANTILYSOZYME ACTIVITY OF MICROORGANISMS - REPRESENTATIVES IN THE TONSILS BIOTOPE OF PATIENTS WITH CHRONIC TONSILLITIS
摘要
The average rate of saliva lysozyme in healthy was 2.1 times higher than in patients. Antilysozyme activity of microorganisms isolated from the healthy biocenosis was 2 times lower antilysozyme activity bacteria from patients. Most often, high values of antilysozyme activity had microorganisms: S. aureus, S. epidermidis and S. warneri.
Russian Journal of Immunology. 2017;20(2):238-240
238-240
RECEPTOR AND PHAGOCYTIC ACTIVITY OF NEUTROPHILS IN CHRONIC DISEASES OF THE UPPER GASTROINTESTINAL TRACT IN CHILDREN, ASSOCIATED WITH HELICOBACTER PYLORI, AND FUNGI OF THE GENUS CANDIDA
摘要
In children with chronic diseases of the upper gastrointestinal tract associated with Helicobacter pylori, and Helicobacter pylori association with fungi of the genus Candida, study of the receptor apparatus (adhesion, to the components of complement-CR 3, Fc fragment of Ig G and activation markers) and phagocytic activity of neutrophils. The identified differentiated characteristics of the phagocytic activity and immunophenotype in Helicobacter pylori infection and association with fungi of the genus Candida. Mannoproteins circulating antigen of Candida albicans affects the immunophenotype of neutrophils, inducing the expression of proapoptotic and reducing the expression of adhesion receptors and cytolysis.
Russian Journal of Immunology. 2017;20(2):241-243
241-243
HOSPITAL-SUBSTITUTION TECHNOLOGIES DURING OPTIMIZATION OF BRONCHIAL ASTHMA REHABILITATION PROGRAMS IN ADOLESCENTS PATIENTS
摘要
Together with the traditional medicinal therapy ispromising the use of nonmedicamental methods of treatment bronchial asthma in the adolescents. The use of hypoxibarotherapy of treatment of adolescents with bronchial asthma led to the positive clinic-immunolologic dynamics. Determination of the immunolologic indexes and the level of the cytokines can be used as the additional test for the evaluation ofits effectiveness.
Russian Journal of Immunology. 2017;20(2):244-246
244-246
THE PROLONGATED EFFECTS OF BETA-ENDORPHIN ON MOUSE IL-1BETA, IL-2, IL-4 AND IL-10 SECRETION IN VIVO
摘要
Beta-endorphin is a compound of peptide nature with a high regulatory activity on the immune system. The existence of long-term immunoregulatory effects of beta-endorphin is of great interest.This work describes the beta-endorphin effects on the corticosterone levels in plasma, as well as the production of key regulatory cytokines by murine splenocytes and macrophages. The dependence of the beta-endorphin effects from the used dose and exposure time was shown.
Russian Journal of Immunology. 2017;20(2):246-248
246-248
DIFFERENTIATION OF T-CELLS UNDER PRIVACY PREGNANCY OF PREGNANCY IN WOMEN OF CHELYABINSK REGION
摘要
Habitual miscarriage of pregnancy (HMP) is one of the leading positions in female infertility. The analysis of T cell differentiation in peripheral blood in patients with HMP has been carried out. A significant increase in the absolute amount of CD45RA+ CD62L+ (Tn) of naive CD4+ and CD8+ lymphocytes was established with a simultaneous increase in the absolute amount of CD45RA-CD62L- (Tem) СD8+ effector cells in patients with HMP compared to the control group. The obtained data require further study and prove the role of the immune system during the gestational process.
Russian Journal of Immunology. 2017;20(2):248-249
248-249
DISTURBANCES OF BONE BLOCKS IN THE OSTEOSYNTHESIS SPINAL DEVICE OF EXTERNAL FIXATION POSSIBLE TO PREDICT
摘要
The purpose of the study have developed a new method of preoperative prediction delayed the formation of a bone block in spinal osteosynthesis external fixation device. The study included 111 patients with vertebral fractures with external fixation osteosynthesis, of whom 16 people were slow fusion of the bone tissue. We investigated the immunophenotyping lymphocyte, functional and metabolic activity of neutrophils, the concentration of the major cytokines and antagonists of their receptors. Determined that preoperative lymphocyte 2,4x109/liter and more associated with slow bone consolidation in more than 85 % of the cases investigated.
Russian Journal of Immunology. 2017;20(2):250-252
250-252
GROW FACTORS IN TRACHEOBRONCHIAL ASPIRATE IN PRETERM INFANTS WITH RDS
摘要
Has been found, that in preterm infants during the development of RDS, the amount of several growth factors is lower than in the control group, but significantly higher in the group of premature infants with lower gestational age. This indicates the processes of consumption of growth factors in the processes of maturation of the bronchopulmonary system, repairing and remodelling, which can result in the development of bronchopulmonary dysplasia and significantly extend the timing of mechanical ventilation.
Russian Journal of Immunology. 2017;20(2):252-254
252-254
THE INFLUENCE OF RECOMBINANT INTERLEUKIN 1-β ON CYTOKINE PROFILE OF MIXED SALIVA OF PATIENTS WITH COMPLICATED FRACTURES OF THE MANDIBLE
摘要
In the research we have analyzed of the cytokine profile of mixed saliva of patients with complicated fractures of the mandible. Studied the levels of proinflammatory and anti-inflammatory cytokines and their dynamics in the treatment process with the conventional schemes and additional treatment with recombinant IL-1β. Indicators of the level of cytokines (Il-1-β, IL-2, Il-4, Il-8, Il-10, and TNFα) in mixed saliva were determined before treatment and 10-12 days of therapy. The study identified the initial increase of proinflammatory cytokines IL-β and IL-8 in all patients, which may indicate the tension of the local immunity in response to trauma. Patients receiving additional immunotherapy, these parameters returned to normal, and level of TNFa was increased, suggesting a high adaptive capacity of the local level of the immune system at use of Betaleukin.
Russian Journal of Immunology. 2017;20(2):254-256
254-256
REACTION OF IMMUNOCOMPETENT CELLS TO KIDNEY ALTERATION IN EXPERIMENTAL ALLOXAN DIABETES
摘要
The reaction of immunocompetent cells to renal alteration was studied on the model of alloxan diabetes in rats. The growing structural violations in the kidney in a chronic hyperglycemia accompanied by increased migration of leukocytes, T-lymphocytes and a decrease in the number of B-lymphocytes and macrophages in the stroma of the organ.
Russian Journal of Immunology. 2017;20(2):257-258
257-258
FAGOTSITARNY ACTIVITY OF MONOCYTES OF THE CENTRAL AND PERIPHERAL LINK OF POSTERITY OF FEMALES OF RATS WITH EXPERIMENTAL DAMAGE OF THE LIVER
摘要
In a research the analysis of features of fagotsitarny ability of monocyte-derived cells of marrow and monocytes of peripheral blood at offspring of females of rats with chronic damage of a liver of toxic genesis with use of microspheres of latex and golden staphylococcus is made (St. aureus). The research has been conducted on white laboratory rats and their posterity during various periods of post-natal ontogenesis. Toxic damage of a liver was modelled by introduction of tetrachloride carbon to mature females. Estimated absorbing ability of cages of a monocyte-derived sprout of marrow and monocytes of peripheral blood. In a monolayer of cages calculation of a fagotsitarny indicator and fagotsitarny index was made. In the course a research oppression of absorbing and Killing activity of cages of a monocyte-derived sprout of marrow and monocytes of peripheral blood of experimental animals is established.
Russian Journal of Immunology. 2017;20(2):259-262
259-262
TO THE PATHOGENESIS OF SPERMATOGENESIS DISORDERS OF THE POSTERITY OF MOTHERS WITH EXPERIMENTAL DEFEAT OF THE HEPATOBILIARY SYSTEM OF VARIOUS GENESIS
摘要
The role of the autoimmune component in the posterity generative function disruption of female rats with chronic liver injury of various genesis was studied. Using immunohistochemical methods it was established that in experimental rats there are inhibition of proliferative and increased apoptotic activities of spermatogenic epithelium, an increase in the titer of antisperm antibodies and a change in the concentration of proinflammatory cytokines. The obtained results allow to consider that the immune component is one of the pathogenetic links of the impairment of the experimental animals fertility.
Russian Journal of Immunology. 2017;20(2):262-264
262-264
POLYMORPHISM OF FAS C14405TGENE AND MARKERS OF CELL DIFFERENCING IN WOMAN OF FERTILE AGE WITH HABITUAL NONCARRYING OF PREGNANCY EXPOSED TO PHENOL
摘要
It has been found that excessive contamination by the phenol in blood of the women of the observation group is associated with: an increase of the relative and absolute CD127- content in comparison to the norm and the control group parameters; a decrease of the absolute and relative CD95+ lymphocytes content relative to the norm and control group. Simultaneously, the C/T genotype of the FAS C14405T gene under conditions of increased phenol content in the blood of women having reproductive disorders significantly enhances the risk of an increase of the relative content of CD127- lymphocytes, which modifies the mechanisms of apoptosis that play a key role in physiological course of the gestational process.
Russian Journal of Immunology. 2017;20(2):265-266
265-266
THE ROLE OF MACROPHAGES IN LOCAL REGULATION OF THE SYNTHETIC ACTIVITY OF INSULIN-SECRECTING PANCREATIC CELLS IN ALLOXANE DIABETES
摘要
On the alloxan diabetes model in rats, it was shown that exposure to monocytes / macrophages with an aminophthalhydroside derived drug leads to a decrease in monocyte infiltration and IL-1a in the pancreas. That promotes the preservation of insulin-producing cells and increases their synthetic activity.
Russian Journal of Immunology. 2017;20(2):267-269
267-269
ASSESSMENT OF PROSPECTS OF COMPLEX APPLICATION OF ANTIBIOTICS AND PROBIOTICS AT TREATMENT OF INFECTIOUS DISEASES
摘要
The antibiotiko produktivnost given on studying and an antibiotiko rezistentnost of pro-biotic strains of bacteria of the sort Bacillus, and studying of effect of combined use of antibiotics and probiotics are presented in article. During the conducted researches patrimonial resistance of bacteria of the sort Bacillus to a tseftazidim, an aztreonam, a colistin and specific stability of B. cereus and B. licheniformis 31 to penicillin, a tsefiksim, a tsefepim, B. subtilis 534 to Chloramfenicolum is established. At the same time the maximal antagonistic activity is registered concerning S. aureus. The most fissile antagonist is B. subtilis 534, and the least fissile B. cereus IP 5832. Studying of effect of combined use in the conditions of in vitro allows to judge the prospect of sharing of B. subtilis 534 and B. subtilis 3 with penicillin, B. licheniformis 31 and tsefiksimy in relation to S. aureus, B. licheniformis 31 with penicillin concerning S. aureus, B. subtilis 534 and B. cereus with tsefiksimy - S. aureus and S. enteritidis, respectively.
Russian Journal of Immunology. 2017;20(2):269-271
269-271
INFLUENCE OF MP-3 ON PRODUCTION OF ACTIVE OXYGEN FORMS, IL-1 β AND TNF-α BY PERITONEAL MACROPHAGES IN PENETRATING EYE INJURY
摘要
Thus, myelopeptide MP-3 has a modulating effect on the function of peritoneal macrophages, eliminating enhancement of IL-1β under penetrating eye injury.
Russian Journal of Immunology. 2017;20(2):271-273
271-273
IMBALANCE OF CHEMOKINES AND GROWTH FACTORS IN THE PATHOGENESIS OF MALE INFERTILITY
摘要
The aim of study was to evaluate the role of chemokines and growth factors in the development of idiopathic infertility. In serum and seminal plasma of the infertile men the deficit and the imbalance of stromal cell factors SDF-1α and transforming growth factor TGFβ, spermatogenesis and modulators of the immune response in the reproductive system are identified. Possible mechanisms for the negative impact of the identified features of the cytokine profile on the fertilizing capacity of sperm were discussed.
Russian Journal of Immunology. 2017;20(2):273-275
273-275
ALLERGIC DISEASES AND HELICOBACTER PYLORI INFECTION PART 1. sIgE HUMORAL IMMUNE RESPONSE TO H. PYLORI
摘要
Serum samples, taken from 12-17 aged children with allergic and concomitant gastrointestinal diseases have been analyzed by ELISA for the total IgE, IL-4, IL-10, IgG, IgA and sIgE to H.pylori. IgE-response to H.pylori prevailed over IgA и IgG Abs and was enhanced in groups of children with allergic diseases and chronic gastroduodenitis, particularly during the exacerbation of allergy. Among children with erosive and ulcerous gastritis and also in group of non-allergic patients with chronic gastroduodenitis, the levels and frequency of sIgE to H.pylori were significantly lower than in healthy controls. The secretion of sIgE to H.pylori is supposed as one of the protective mechanisms under the conditions of chronic mucosal inflammation, associated with favorable outcomes of gastrointestinal diseases.
Russian Journal of Immunology. 2017;20(2):275-277
275-277
ALLERGIC DISEASES AND HELICOBACTER PYLORI INFECTION PART 2. sIgE MUCOSAL IMMUNE RESPONSE TO H. PYLORI
摘要
The levels of sIgA, CagA, IL-17, sIgE-to H.pylori and to CagA have been analyzed by ELISA in the samples of coprofiltrates, taken from children with allergy and concomitant gastrointestinal diseases. Anti-H. pylori and anti-CagA sIgE have been elevated in groups of patients with allergic diseases in combination with chronic gastroduodenitis and among children with erosive and ulcerous leisons, being the most significant among the last group. Non-allergic patients with chronic gastroduodenitis were characterized by the highest concentration of IL-17 and sIgA, which was associated with the confirmed H.pylori infection. Our data demonstrates that sIgE to H.pylori, together with sIgA, represent a part of protective mechanisms during the H.pylori -assosiated gastrointestinal pathology.
Russian Journal of Immunology. 2017;20(2):278-280
278-280
CONDITION OF THE IMMUNE SYSTEM ORGANS AND BLOOD LEUCOCYTES IN RATS AFTER THE EXPOSITION OF IRON-MOLYBDENUM POLYOXOMETALATES
摘要
When iron-molybdenum polyoxometalates (POM) were introduced to rats, the accumulation of heat shock proteins (HSP) in the thymus and spleen were revealed without altering the histological structure of the organs. This finding indicates both the stressing impact of the POM and the protective action of the HSP. Lymphocyte apoptosis increase and H1 histone protein increase are supposed to be indicators preceding the destructive changes in immunopoietic organs.
Russian Journal of Immunology. 2017;20(2):280-282
280-282
EVALUATION AND CORRECTION OF IMMUNE STATUS, LIPID PEROXIDATION SYSTEM IN PATIENTS WITH RECURRENT HERPES VIRUS INFECTION OF GENITAL
摘要
The influence of intravascular laser therapy on the immune status and free radical oxidation processes in patients with chronic recurrent herpesvirus infection. Laser exposure parameters: wavelength 635 nm, continuous mode, power density 0.12 mW / cm2, exposure 8 minutes, the number of procedures is 10. In the course of the study, it was established that in patients with chronic recurrent herpes viral infection, the parameters of the neutrophilic granulocyte test are increased, the phagocytic activity decreases, the serum level of interleukin-2 decreases, and the levels of all categories of lipid peroxidation products in the blood serum increase. The detected immunological and biochemical disorders were successfully corrected by a complex of therapeutic measures using the intravascular laser irradiation method
Russian Journal of Immunology. 2017;20(2):283-284
283-284
THE REGULATION OF TREG DIFFERENTIATION BY ENDOGENOUS MELATONIN DURING PREGNANCY
摘要
The role of endogenous melatonin in the regulation of the process of regulatory T cells (Treg) differentiation during the gestation period has been studied. The difference in the regulation of this process by the hormone was shown in non-pregnant and pregnant women. The positive effect of melatonin on the cells in vitro mediated by signals from membrane receptors for the hormone in response to polyclonal activation was revealed at pregnancy.
Russian Journal of Immunology. 2017;20(2):285-286
285-286
CD39 AND CD73 EXPRESSION IN INTERSTITIAL CELLS OF CALCIFIED AORTIC STENOSIS
摘要
Investigation of CD39 and CD73 expression on interstitial cells of calcified aortic valves of 31 patients was performed. Not significant increase (p=0,45) of CD39-CD73+ in cells from tricuspid aortic valves (TAV) comparing with bicuspid valves (BAV) was shown. Only from female patient's valves the difference became significant (BAV - 23,67 (19,43;29,80)%; TAV - 59,34 (49,17;69,55)% (p=0,002)). These data proves the hypotheses of purinergic signaling involvement in calcification suggesting that it is sex related process.
Russian Journal of Immunology. 2017;20(2):287-289
287-289
ROLE OF HOMOSERINLACLONTS IN SALMONELLA SURVIVAL DURING PHAGOCYTOSIS
摘要
Described increase in the virulence of Salmonella in the presence of small regulatory molecules. The influence of homoserine, among which the most important role played by N-(3-oxo-hexanoyl)-homos-erine lactone, on the induction of the promoter rck the strain S. typhimurium LT2, which determines the resistance of the complement system. Revealed increased resistance of the strain to phagocytosis in the presence of homoserine.
Russian Journal of Immunology. 2017;20(2):289-291
289-291
MANIFESTATIONS OF PRIMARY IMMUNODEFICIENTS IN THE REGION OF THE MOUTH AND THE MAXIMUM-FACE REGION
摘要
The aim of the work was to describe the nature of various orofascial manifestations of primary immunodeficiency in patients in the register of the Sverdlovsk Region in comparison with data from literature sources. As a result of the study, it was possible to trace the significant correspondence of the detected pathological manifestations with identical PID groups. The most common signs can be called retention of baby teeth, candidiasis of the oral mucosa, angular cheilitis, multiple caries in combination with manifestations of primary immunodeficiency in other organs and systems.
Russian Journal of Immunology. 2017;20(2):291-294
291-294
DYNAMICS OF CHANGES FUNCTIONAL PARAMETERS OF NEUTROPHILS IN SEPTIC PATIENTS WITH A FAROBLE OUTCOME
摘要
The study evaluated the functional activity of neutrophils in septic patients with a faroble outcome. In patient with sepsis there were determined increase in all parameters functional activity of neutrophils in first day of diagnostics sepsis. It was established that in the dynamics with improved clinical status, these parameters are falling.
Russian Journal of Immunology. 2017;20(2):295-296
295-296
LOCAL IMMUNITY FACTORS IN PREGNANT WOMEN WITH INTRAUTERINE INFECTION
摘要
The aim of our study was to study local immunity factors in pregnant women with and without morphologically verified intrauterine infection and to identify changes that can mark its implementation during pregnancy. We established that local immunity in pregnant women with intrauterine infection had increased proinflammatory capacity of cervical mucus at one hand and on the other hand had functional activity reduction in particular innate immunity factors.
Russian Journal of Immunology. 2017;20(2):296-298
296-298
IMMUNOLOGICAL INDICATORS OF CERVICAL MUCUS AND ENDOMETRIAL SECRET VALUE FOR CHONIC ENDOMETRITIS DIAGNOSIS
摘要
We conducted a cross-sectional study of 205 patients in reproductive age with morphologically verified chronic endometritis. We researched concentration of bactericidal factors according to inflammatory activity degree chronic endometritis. We established α-defensin, BPI, sTREM-1 in cervical mucus can reflect chronic endometritis activity degree that can be basis for efficiency evaluation of chronic endo-metritis treatment without control endometrium biopsy.
Russian Journal of Immunology. 2017;20(2):299-301
299-301
THE ACTIVITY OF THE SYNTHESIS OF THE CYTOKINES BY MACROPHAGES IN THE PROCESS OF THE PHAGOCYTOSIS IN VITRO UNDER THE EFFECT BY LECTIN LACTOBACILLUS DELBRUECKII SSP. BULGARICUS
摘要
Studied the effect of lectin Lactobacillus delbrueckii ssp. bulgaricus on cytokine profile of mice during the process of phagocytosis in vitro. Presented studies indicate lectin-specific interaction with surface structures of peritoneal macrophages and has allowed to establish that the lectin of L. delbrueckii ssp. bul-garicus in a greater degree caused induction of the synthesis of interleukin-1α by phagocytes.
Russian Journal of Immunology. 2017;20(2):301-303
301-303
EFFECT OF IRON NANOPARTICLES ON THE BLOOD CHEMISTRY AND INDICES OF NONSPECIFIC IMMUNITY OF AGRICULTURAL BIRDS
摘要
The issues of the effect of iron nanoparticles on biochemical blood indices and nonspecific immunity parameters were studied. It was demonstrated that the introduction of iron nanoparticles into the diet of animals did not exert its own influence on the biochemical parameters of the blood. However, it negatively affects the body of the bird being studied, as evidenced by an increase in these groups of concentrations of alkaline phosphatase and aminotransferases. The study of the dynamics of the leukocyte profile revealed only insignificant fluctuations of the studied parameters within the limits of the physiological norm.
Russian Journal of Immunology. 2017;20(2):303-305
303-305
COMPARISON OF CHELYABINSK REGION TATARS WITH POPULATIONS OF EURASIA ON TLR 1 GENE SINGLE NUCLEOTIDE POLYMORPHISM 1805T>G
摘要
In this article the frequency of toll-like receptor 1 gene 1805T>G single nucleotide polymorphism in Tatars of Chelyabinsk Region has been compared with some Eurasian populations. The genotyping of the South Ural Tatars on the toll-like receptor 1 gene 1805T>G was conducted for the first time. Obtained data can be used for the further study of the infection response in different populations.
Russian Journal of Immunology. 2017;20(2):306-308
306-308
COMPARATIVE CHARACTERISTIC OF LABORATORY METHODS OF DIAGNOSTICS OF UROGENITAL INFLAMMATORY DISEASES
摘要
The effectiveness of cultural and immunological methods of diagnosis of urogenital inflammatory diseases is analyzed. The expediency of using the immunological method for identifying groups of patients requiring an extended microbiological examination is proved.
Russian Journal of Immunology. 2017;20(2):308-310
308-310
CHANGES OF CYTOKINE LEVELS IN PATIENTS WITH REACTIVE ARTHRITIS, FOR THE THERAPY OF POTENTIATED ANTIBODIES TO TUMOR NECROSIS FACTOR-ALPHA
摘要
Patients Rea identified changes in the levels of proinflammatory and anti-inflammatory cytokines in the blood plasma before and after the appointment of therapy potentiated antibodies to tumor necrosis factor alpha, as well as indicators of clinical, inflammatory, and functional activity. The use of artrofoon provide significant reduction of Pro-inflammatory cytokines and normalisation of anti-inflammatory.
Russian Journal of Immunology. 2017;20(2):310-312
310-312
CHARACTERISTICS OF SYSTEMIC IMMUNITY IN WOMEN WITH ENDOMETRIOSIS AND GENITAL INFECTION
摘要
The study of the indices of systemic immunity in women with endometriosis, depending on the presence or absence of pathogens of genital infection. Pathogens of genital infection were found in 50 % of women with endometriosis in endocervix, endometrium, peritoneal fluid and in the foci of endometriosis. The most commonly identified were Ureaplasma spp, HPV and CMV. Changes in the indices of systemic immunity in women with endometriosis in comparison with the control group were more pronounced in the presence of pathogens of genital infection.
Russian Journal of Immunology. 2017;20(2):312-314
312-314
CHARACTERISTICS OF CYTOKINEMIA IN VARIOUS AUTOIMMUNE DISEASES
摘要
The article presents the results of comparative analysis of cytokinemia (IL-6, IL-8, IL-10, TNFα) in various autoimmune diseases: autoimmune thyroiditis, valvular heart disease, psoriatic arthritis, rheumatoid arthritis, reactive arthritis, ankylosing spondylitis, chronic glomerulonephritis with end-stage renal disease, systemic lupus erythematosus. Severity of cytokine response was maximal in systemic lupus erythematosus and chronic glomerulonephritis. The greatest deviations from normal values in these autoimmune diseases were observed in TNFα and IL-8 levels.
Russian Journal of Immunology. 2017;20(2):315-316
315-316
MOLECULAR MODELLING OF FLAVONOID QUERCETIN TARGETING TO THE TOLL-LIKE RECEPTORS
摘要
Molecular modelling of quercetin targeting to the extracellular and cytosolic domains of human Tolllike receptors (TLR1-TLR10) was performed. We showed that quercetin can be targeted to various TRLs preferably either in extracellular domain sites (TLR4, TLR5, TLR7, TLR8) or in cytosolic domain sites (TLR1-TLR2, TLR2-TLR6).
Russian Journal of Immunology. 2017;20(2):317-319
317-319
HORIONIC GONADOTROPIN IN REGULATION OF THE FUNCTIONAL ACTIVITY OF T-REGULATORY LYMPHOCYTES
摘要
The effect of human chorionic gonadotropin (HCG) on apoptosis and production of IL-10 and TGF-β1 by isolated pools of natural T-regulatory lymphocytes (nTreg, CD4+CD25bright) and precursors of adaptive Treg (aTreg, CD4+CD25-) in the in vitro system was studied. As a result, we demonstrated that HCG induces apoptosis of nTreg and interferes with aTreg apoptosis, without affecting the production of IL-10 and TGF-β1 by these cells.
Russian Journal of Immunology. 2017;20(2):319-321
319-321
THE DISTRIBUTION OF GENES AND HAPLOTYPES HLA II CLASS IN NAGAIBAKS POPULATION IN COMPARISON WITH OTHER POPULATIONS OF THE CHELYABINSK REGION AND EURASIA
摘要
The issues of populational immunogenetics are described in this article. In particular, the comparative characteristic of the frequencies of HLA II class genes and haplotypes in Nagaibaks population with the main populations of the Chelyabinsk region (Russian, Tatars and Bashkirs) is analyzed. Genetic distances on HLA II class genes between Nagaibaks population and some populations of Eurasia are constructed. The results of our research show, that population Nagaibaks is largely different from the population of Bashkirs of Chelyabinsk region on the frequencies of genes and haplotypes of HLA II class. According to the assessment of genetic distances on the HLA II class genes populations of Russian, Tatar population of the Chelyabinsk region and the Chuvash are the nearest to Nagaibaks population.
Russian Journal of Immunology. 2017;20(2):321-323
321-323
INFLUENCE OF OXIDATIVE STRESS ON POSTOPERATIVE PERIOD OF DIABETIC CATARACT SURGERY
摘要
The purpose of this study is to reveal the state of the body's antioxidant systems using Western-blot enzyme immunoassay in tears for peroxedoxin-6 (PRDX6) proteins and / or fragments of its disintegration as a marker of protection against oxidative stress in patients with age and diabetic cataracts. Patients and methods. 136 patients (136 eyes) with cataract of various genesis were examined. The control group was represented by 25 volunteers who did not have eye diseases (50 eyes). Two groups of patients were examined. The first group included 78 patients with age-related cataracts. The second group included 58 patients with diabetic complicated cataract. The material of the study was fluids of the tear fluid the of the anterior chamber of patients with cataracts. To determine the active peroxedoxin-6 (PRDX6) and / or its degradation fragments, a Western-blot enzyme-linked immunosorbent assay was used. The expression of enzymes of protection against oxidative stress in samples of tear fluid was studied and a comparative evaluation of the level of activity of lactic acid antioxidant enzymes in oxidative stress after cataract surgery was given. Results. In the postoperative period in patients with age-related cataracts, the increase in the level of PRDX6 is determined, which is confirmed by the absence of complications of FEC. With a complicated cataract, the PRDX6 content is 6 times lower than in patients with age-related cataracts. Conclusion. If there is an active peroxiredoxin 6 in the lacrimal fluid and / or fragments of its decay, age-related cataracts are diagnosed, and this corresponds to a high level of protection against oxidative stress. In such patients, the postoperative period proceeds favorably. In the absence of active peroxiredoxin-6 and / or fragments of its decay in the lacrimal fluid, the presence of cataract due to diabetes mellitus is diagnosed. In the study of anterocameral fluid, a marker of development of metabolic disturbances in complicated cataracts and a 45 kDa transporter of hydrophobic compounds of the family of Sec-14p-like proteins was revealed in patients of group 2. Cataracts are considered diabetic, and in the postoperative period, the risk of developing PEER increases. The use of the method makes it possible to increase the accuracy of the choice of cataract treatment tactics.
Russian Journal of Immunology. 2017;20(2):324-330
324-330
THE PREVALENS OF PERTUSSIS IN CHILDREN IN CHELYABINSK 2007-2015 YEARS AND POSTVACCINAITION IMMUNITI AGAINST PERTUSSIS
摘要
The pertussis infection is a general problem. The presents data on the incidence of pertussis in children in Russia, in Chelyabinsk region and Chelyabinsk 2007-2015 years. Analisis of the effectiveness pertussis mmunization. As part of investigation immune system and protective pertussis antibodies in children. Identified changes postvaccination immunity may indicate ineffective respons to vaccination against pertussis, as well as the inability to from immunological memory, prolonged post-vaccination antibody.
Russian Journal of Immunology. 2017;20(2):331-333
331-333
ANALYSIS OF INTERDEPENDENCE OF INDICATORS OF LOCAL IMMUNITY DEPENDING ON THE DURATION OF HPV INFECTION
摘要
The analysis of interrelations between cytokines and immunological indices of cervical mucus with transient and persistent HPV infection was carried out. With transient HPV infection, the balance of pro- and anti-inflammatory cytokines is maintained. With persistent HPV infection for 6 months, activation of local protective reactions aimed at involving effector cells in the pathogen elimination process was noted. With persistent HPV infection for 12 months, there is a change in the direction of immunological regulation towards anti-inflammatory cytokines.
Russian Journal of Immunology. 2017;20(2):333-335
333-335
CYTOKINES' RESPONSE UNDER SEPSIS AND TRAUMA
摘要
We detected rates of IL-6, -8, -10, TNF-α, and CRP in plasma of patients with sepsis and polytrauma at 1-2nd and 5-7th days after admission in ICU. All patients were divided into groups with/ without MODS development. A rise in mediator levels in all groups of patients were identified. The maximum of detected increasing was in patients with MODS. There was not a clear cytokines-etiological agent dependence.
Russian Journal of Immunology. 2017;20(2):336-337
336-337
INFLUENCE OF INTESTINE MICROSYMBIONTS METABOLITES ON THE PRODUCTION OF NITRIC OXIDE BY PERITONEAL MACROPHAGES
摘要
Metabolites of clinical cultures Clostridium spp., Staphylococcus spp., Bacteroides spp. significantly inhibited in vitro basal secretion by peritoneal macrophages (MF) of nitric oxide (NO) mice. In contrast, metabolites of Bifidobacterium spp., Lactobacillus spp. and Propionibacterium spp. moderately stimulated the NO secretion. The overall effect of the metabolites influence of intestinal microsymbionts on the functional activity of MF will be determined by their qualitative and quantitative composition in the human intestine eubiosis and dysbiosis.
Russian Journal of Immunology. 2017;20(2):338-340
338-340
IDIOTYPICAL MECHANISMS OF INDUCTION AND REGULATION OF AUTOIMMUNE REACTIONS IN EXPERIMENTAL AUTOIMUNE ENTSEPHALOMYELITIS IN RATS
摘要
The aim of research was to confirm that induction and regulation of autoreactive lymphocytes in model of the experimental autoimmune encephalomyelitis (EAE) in rats is carried out indirectly through idiotip-antiidiotipic (IAI) interactions with participation of the lymphocytes producing antiidiotipic antibodies with rheumatoid factors (RF) properties. Methods. Rats were immunized by the main guinea pig myelin protein (MMPgp). The kinetics of antibodies and autoantibodies formation during the immune response was investigated. MMPgp antibodies and MMP rats autoantibodies were determined by ELISA. RF was determined during agglutination test of tannised loaded IgG erythrocytes. The presence of IAI interactions was studied by competitive ELISA method. Results. Existence of IAI interactions between MMPgp antibodies and RF on the one hand, and, between MMP rats antibodies and RF on another hand was demonstrated. Association between RF high level in a blood and lack of clinical signs of EAE was found in immunized animals. Conclusion. The mechanism of induction of MMP rats autoantibodies during MMPgp immunization is mediated by IAI interactions of that clones of lymphocytes, which producing antiidiotipic antibodies with RF properties. Antiidiotipic antibodies with RF properties is carry out a specific negative regulation of a humoral immune response against a heterological antigen inductor of EAE, MMPgp through IAI interactions.
Russian Journal of Immunology. 2017;20(2):340-342
340-342
CYTOKINE PROFILE OF MICE IMMUNIZED BY COMPLEX OF RECOMBINANT OPRF AND ATOX ANTIGENS OF PSEUDOMONAS AERUGINOSA
摘要
Currently, infections caused by Pseudomonas aeruginosa are an important medical problem. Their treatment is extremely difficult because of the broad antibiotic resistance of the pathogen. Objective: to study the effect of immunization with a recombinant antigens of Pseudomonas aeruginosa on the concentration of cytokines in the serum of mice. Materials and methods. Mice were injected intraperitoneally with recombinant 25 μg OprF and 50 μg of toxoid. The cytokine responses of mice were monitored by using flow cytometry analysis at 2, 4, 6 and 24 hours after immunization («FlowCytomix Mouse Th1/Th2 10 plex»). Results. The dynamics of the cytokine levels in the sera of mice was revealed: after 4 hours IL-1α, IL-5; 8 hours later, Π,-1β, IL-2, IL-4, and Th17/Th21/Th22 cytokines-IL-17A and IL-22 and IL-21; 24 hours later, IL-6, IL-10, IL-12p70, TNF-a and IFN-γ. Conclusion. Candidate vaccine against Pseudomo-nas aeruginosa based on the recombinant proteins OprF and aTox activates both cellular and humoral immune system with induction of Th1, Th2 and Th17 responses.
Russian Journal of Immunology. 2017;20(2):343-345
343-345
MOLECULAR FACTORS ROLE IN FORMING SERUM BLOOD BACTERICIDE PROPERTIES IN RESPECT OF LUMINESCENT STRAINS
摘要
Molecular components of blood serum have multidirectional effects on different groups of bacteria. In the work strains with two types of cell wall and ecological features were used. The role of immunoglobulins and C 5 protein of the complement system in the intensity of bactericidal effect against luminescent bacteria was determined.
Russian Journal of Immunology. 2017;20(2):345-347
345-347
STUDY OF THE BIOLOGICAL AND MECHANICAL PROPERTIES OF THE ULTRA-HIGH-MOLECULAR-WEIGHT-POLY-ETHYLENE COMPOSITE FOR EMPLOYMENT AS CARTILAGE BIOIMPLANT
摘要
Aim of this study is researching the biological and mechanical features of the material, made of ultra-high-molecular-weight-poly-ethylene(UHMWPE) Studing the mechanical features showed that the material prepared with addition of Al2O3 and with using mechanical activation has the highest mechanical features, wear resistance in particular, in comparison with UHMWPE samples without Al2O3 or prepared without mechanical activation. was chosen for. After 60 days orthotopical transplantation this material in rats we did not fined signs of inflammation, cellular infiltration, destruction of material or bone-cartilage defect. Sample was in position of implantation without any shifts. Acquired data shows the perspective-ness of UHMWPE as a material for creating bioimplants for cartilage defects replacement.
Russian Journal of Immunology. 2017;20(2):347-350
347-350